Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PUN50_RS02905 Genome accession   NZ_CP117988
Coordinates   597814..599037 (-) Length   407 a.a.
NCBI ID   WP_045451199.1    Uniprot ID   A0AAQ2XYJ9
Organism   Vibrio campbellii strain 20220413_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 592814..604037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN50_RS02870 (PUN50_02870) rimM 593037..593585 (+) 549 WP_005534055.1 ribosome maturation factor RimM -
  PUN50_RS02875 (PUN50_02875) trmD 593613..594356 (+) 744 WP_005534054.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  PUN50_RS02880 (PUN50_02880) rplS 594398..594751 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  PUN50_RS02885 (PUN50_02885) yacG 595232..595426 (-) 195 WP_005534053.1 DNA gyrase inhibitor YacG -
  PUN50_RS02890 (PUN50_02890) zapD 595489..596229 (-) 741 WP_012128881.1 cell division protein ZapD -
  PUN50_RS02895 (PUN50_02895) coaE 596257..596871 (-) 615 WP_045369833.1 dephospho-CoA kinase -
  PUN50_RS02900 (PUN50_02900) pilD 596872..597741 (-) 870 WP_274290832.1 prepilin peptidase Machinery gene
  PUN50_RS02905 (PUN50_02905) pilC 597814..599037 (-) 1224 WP_045451199.1 type II secretion system F family protein Machinery gene
  PUN50_RS02910 (PUN50_02910) pilB 599072..600757 (-) 1686 WP_274290833.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PUN50_RS02915 (PUN50_02915) - 600757..601224 (-) 468 WP_274290834.1 pilin -
  PUN50_RS02920 (PUN50_02920) nadC 601490..602377 (-) 888 WP_012128875.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PUN50_RS02925 (PUN50_02925) ampD 602470..603021 (+) 552 WP_038891990.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44883.09 Da        Isoelectric Point: 10.3971

>NTDB_id=788958 PUN50_RS02905 WP_045451199.1 597814..599037(-) (pilC) [Vibrio campbellii strain 20220413_1]
MKTIAPQLKNYRWKGINSSGKKTSGNVLAMTEVEVRERLDAQHIKIKKLKKGSISFITKISHRVKGKDITIFTRQIATML
MTGVPIVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASTHFDPLYTDLIATGEQSGNLSQVFERLATYREK
SEQLRAKVIKALIYPAMVVLVALGVSYLMLTRVIPEFEKMFTGFGADLPAFTQMVLNLSAWTQNWGPFIGLSSVSLLISG
KIMSQRSDSFRLLAARSSLRVPILGPVLSKAAIAKFSRTLATSFSAGIPILTALKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYIAMRNCNVFPELVLQMVMIGEESGRLDDMLNKIAAIYEFEVDNTVDNLSKILEPLIIVFLGVVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=788958 PUN50_RS02905 WP_045451199.1 597814..599037(-) (pilC) [Vibrio campbellii strain 20220413_1]
ATGAAAACCATCGCTCCACAACTTAAGAACTACCGTTGGAAAGGCATTAATAGCTCGGGTAAAAAAACCTCTGGAAACGT
GCTCGCCATGACAGAAGTCGAGGTAAGAGAGCGCCTCGATGCTCAACACATCAAGATAAAGAAACTGAAGAAAGGCAGCA
TTTCTTTTATCACCAAGATCAGTCATCGCGTCAAAGGCAAAGACATCACGATATTTACTCGCCAAATTGCCACCATGCTA
ATGACGGGCGTACCGATTGTTCAGGCACTTAAGTTGGTTTCAGAGAACCACAAAAAAGCCGAGATGAAATCGATTCTGAT
GAGTGTCACAAGAGCGGTAGAGGCAGGGACACCGATGTCGAAAGCCATGCGCACTGCCAGTACACATTTTGATCCGCTTT
ATACAGACCTAATCGCGACTGGTGAGCAGTCCGGTAACCTATCTCAAGTCTTTGAGCGCTTGGCAACTTATCGAGAAAAG
AGCGAGCAGTTGCGCGCAAAAGTGATTAAAGCGCTCATTTACCCAGCGATGGTGGTTTTAGTAGCGCTTGGGGTTTCCTA
TTTAATGCTGACTAGAGTTATCCCTGAGTTTGAAAAGATGTTTACAGGTTTTGGAGCCGACTTACCCGCCTTTACTCAGA
TGGTACTCAACCTCTCTGCTTGGACCCAAAACTGGGGCCCCTTTATTGGACTGAGTTCTGTGAGCCTGCTCATTTCCGGC
AAAATTATGTCGCAACGATCCGATTCATTTCGCCTTTTAGCCGCGCGTTCGAGTTTAAGAGTCCCTATCTTGGGGCCTGT
ATTATCCAAAGCAGCCATTGCCAAGTTTAGCCGTACTTTAGCAACCAGCTTTAGTGCGGGTATTCCAATTCTCACCGCAT
TAAAAACCACCTCAAAAACCTCCGGTAACATGCACTATCAATTAGCAATTGAAGAAGTGTATCGAGACACTGCAGCTGGT
ATGCCGATGTATATTGCCATGCGTAACTGTAACGTTTTCCCTGAGCTAGTACTGCAAATGGTCATGATAGGTGAAGAGTC
AGGTCGATTGGATGACATGCTCAATAAGATAGCCGCTATTTATGAGTTCGAAGTGGATAATACCGTTGATAATCTGAGTA
AGATTTTAGAACCATTGATCATCGTCTTTCTTGGCGTAGTGGTTGGTGGTCTAGTCACAGCAATGTACTTACCAATCTTT
AATTTGATGAGCGTATTAGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

99.017

100

0.99
  pilC Vibrio cholerae strain A1552

73.75

98.28

0.725
  pilC Acinetobacter baylyi ADP1

42.714

97.789

0.418
  pilC Acinetobacter baumannii D1279779

40.695

99.017

0.403
  pilC Pseudomonas stutzeri DSM 10701

40.657

97.297

0.396
  pilC Legionella pneumophila strain ERS1305867

39.356

99.263

0.391
  pilG Neisseria gonorrhoeae MS11

38.765

99.509

0.386
  pilG Neisseria meningitidis 44/76-A

38.177

99.754

0.381