Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PUN50_RS02910 Genome accession   NZ_CP117988
Coordinates   599072..600757 (-) Length   561 a.a.
NCBI ID   WP_274290833.1    Uniprot ID   A0AAQ2Y0Y0
Organism   Vibrio campbellii strain 20220413_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 594072..605757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN50_RS02880 (PUN50_02880) rplS 594398..594751 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  PUN50_RS02885 (PUN50_02885) yacG 595232..595426 (-) 195 WP_005534053.1 DNA gyrase inhibitor YacG -
  PUN50_RS02890 (PUN50_02890) zapD 595489..596229 (-) 741 WP_012128881.1 cell division protein ZapD -
  PUN50_RS02895 (PUN50_02895) coaE 596257..596871 (-) 615 WP_045369833.1 dephospho-CoA kinase -
  PUN50_RS02900 (PUN50_02900) pilD 596872..597741 (-) 870 WP_274290832.1 prepilin peptidase Machinery gene
  PUN50_RS02905 (PUN50_02905) pilC 597814..599037 (-) 1224 WP_045451199.1 type II secretion system F family protein Machinery gene
  PUN50_RS02910 (PUN50_02910) pilB 599072..600757 (-) 1686 WP_274290833.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PUN50_RS02915 (PUN50_02915) - 600757..601224 (-) 468 WP_274290834.1 pilin -
  PUN50_RS02920 (PUN50_02920) nadC 601490..602377 (-) 888 WP_012128875.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PUN50_RS02925 (PUN50_02925) ampD 602470..603021 (+) 552 WP_038891990.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PUN50_RS02930 (PUN50_02930) pdhR 603426..604193 (+) 768 WP_005428674.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62118.86 Da        Isoelectric Point: 5.1854

>NTDB_id=788959 PUN50_RS02910 WP_274290833.1 599072..600757(-) (pilB) [Vibrio campbellii strain 20220413_1]
MHTNLSTVLRQAGILTFSQEESLLEQVKASGISMPEALLSSGFFQSHELTEHLSSVFGLSCTSLNQYEYASLCQTLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRSLGQEKSGLKEINQDELAG
LVDIGEDEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPTHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDSQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPYCRQPQEPNSQLQHIGITSTEQ
IFQANPDGCNECTHGYSGRTGIYEVMKFDESLSEALIKGASVHELEKLAIANGMQTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=788959 PUN50_RS02910 WP_274290833.1 599072..600757(-) (pilB) [Vibrio campbellii strain 20220413_1]
ATGCACACAAACCTCTCAACGGTTCTTCGCCAAGCAGGAATACTGACCTTTTCCCAAGAAGAATCGTTACTTGAACAAGT
AAAAGCTTCTGGCATTTCGATGCCGGAGGCTTTACTCAGTTCTGGCTTTTTCCAATCTCATGAATTAACAGAACACCTAA
GCTCAGTATTCGGGCTTTCTTGCACTTCTCTTAACCAATACGAATATGCTTCGCTATGCCAAACGCTTGGCTTACGCGAT
CTCATCACTCGCCATAACGCTTTGCCGCTTAACCGCACCTCTTCAACTTTGATTCTTGCTGTTGCCGACCCAACAAACCT
TCAAGCCGAAGATGACTTTCGTTTTGCGACAGGTTTGCAAGTCGAACTTGTCCTCGCTGACTTTCGCGAACTGACAGCAG
CCATTCGGCGTTTATATGGCCGATCGCTTGGGCAAGAAAAATCCGGTCTTAAAGAAATCAACCAAGATGAACTGGCGGGT
TTGGTTGATATTGGTGAGGATGAAATTGAGAACATCGAAGATCTCAGCCAAGACGAATCGCCGGTCAGTCGCTATATCAA
CCAAATTTTACTCGATGCTGTCCGTAAAGGGGCTTCCGATATTCACTTCGAGCCTTACGAAAATATGTACCGCGTGCGCT
TGCGTTGCGATGGCATCTTAATTGAAATTCAGCAGCCTCCGACTCATTTAAGCCGTCGCCTTTCAGCTCGGATAAAAATC
TTATCTAAGCTTGATATCGCTGAGCGCCGTTTACCACAGGATGGACGCATCAAGCTAAAATTAAACCAAGACACTGCAAT
TGATATGCGTGTCTCCACACTACCAACTCTTTTTGGGGAAAAGATTGTTCTGCGTTTGCTCGACAGCAGCTCTGCAGCAT
TAGATATCAATAAGCTCGGTTATAGCGACTCACAAAAACAACTTTATTTGGATGCCCTGCGCCGTCCGCAAGGTATGATC
CTGATGACAGGCCCAACAGGTAGCGGTAAAACGGTCTCGCTCTACACTGGATTGAGCATTCTTAATAAACCAGAGATCAA
TATCTCAACCGCTGAAGATCCCGTTGAGATTAACCTGCCCGGCATTAACCAAGTTCAAGTTCAGCCAAAGATCGGCTTTG
GTTTTGCAGAAGCGCTCCGCTCTTTCTTACGTCAAGACCCTGATGTCGTCATGGTGGGCGAGATCCGAGATCTCGACACA
GCAGAAATCGCGATCAAAGCATCACAGACTGGCCATTTAGTTCTCTCCACTTTACACACAAACTCAGCTGCCGAGACTGT
CATTCGCTTGTCTAATATGGGCGTAGAAAGCTTTAACCTCGCCTCTTCTCTCAGTTTGATCATCGCCCAGCGTTTGGCTA
GAAAACTTTGTCCATATTGTAGACAACCTCAAGAGCCAAACAGCCAACTTCAACATATTGGAATCACCAGCACCGAACAG
ATCTTTCAAGCTAATCCCGACGGTTGTAACGAGTGTACCCATGGTTATTCAGGGCGAACCGGCATTTACGAAGTGATGAA
GTTTGACGAATCCCTTTCAGAAGCACTCATCAAAGGCGCTTCAGTACACGAATTAGAAAAACTCGCCATTGCCAATGGTA
TGCAAACCCTACAAATGTCTGGCATCGAAAAACTCAAACAAGGCATTACCAGCTTCAGCGAATTGCAGCGCGTTCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

98.93

100

0.989
  pilB Vibrio parahaemolyticus RIMD 2210633

90.909

100

0.909
  pilB Vibrio cholerae strain A1552

73.31

100

0.734
  pilB Legionella pneumophila strain ERS1305867

47.756

99.287

0.474
  pilB Acinetobacter baumannii D1279779

51.252

92.513

0.474
  pilB Acinetobacter baylyi ADP1

50.29

92.157

0.463
  pilF Neisseria gonorrhoeae MS11

43.617

100

0.439
  pilB/pilB1 Synechocystis sp. PCC 6803

37.205

100

0.394
  pilF Thermus thermophilus HB27

36.833

100

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.559

99.465

0.364