Detailed information NTG0788957

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PUN50_RS02900
• Genome accession: NZ_CP117988
• Coordinates: 596872..597741 (-)
• Length: 289 a.a.
• NCBI ID: WP_274290832.1
• Uniprot ID: A0AAQ3B0M2
• Organism: Vibrio campbellii strain 20220413_1
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 591872..602741

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUN50_RS02865 (PUN50_02865)	rpsP	592761..593009 (+)	249	WP_005438039.1	30S ribosomal protein S16	-
PUN50_RS02870 (PUN50_02870)	rimM	593037..593585 (+)	549	WP_005534055.1	ribosome maturation factor RimM	-
PUN50_RS02875 (PUN50_02875)	trmD	593613..594356 (+)	744	WP_005534054.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
PUN50_RS02880 (PUN50_02880)	rplS	594398..594751 (+)	354	WP_005379971.1	50S ribosomal protein L19	-
PUN50_RS02885 (PUN50_02885)	yacG	595232..595426 (-)	195	WP_005534053.1	DNA gyrase inhibitor YacG	-
PUN50_RS02890 (PUN50_02890)	zapD	595489..596229 (-)	741	WP_012128881.1	cell division protein ZapD	-
PUN50_RS02895 (PUN50_02895)	coaE	596257..596871 (-)	615	WP_045369833.1	dephospho-CoA kinase	-
PUN50_RS02900 (PUN50_02900)	pilD	596872..597741 (-)	870	WP_274290832.1	prepilin peptidase	Machinery gene
PUN50_RS02905 (PUN50_02905)	pilC	597814..599037 (-)	1224	WP_045451199.1	type II secretion system F family protein	Machinery gene
PUN50_RS02910 (PUN50_02910)	pilB	599072..600757 (-)	1686	WP_274290833.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PUN50_RS02915 (PUN50_02915)	-	600757..601224 (-)	468	WP_274290834.1	pilin	-
PUN50_RS02920 (PUN50_02920)	nadC	601490..602377 (-)	888	WP_012128875.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32502.81 Da
• Isoelectric Point: 8.1362

>NTDB_id=788957 PUN50_RS02900 WP_274290832.1 596872..597741(-) (pilD) [Vibrio campbellii strain 20220413_1]
MEVFQYYPWLFVVFATIFGLIVGSFLNVVIYRLPKIMELEWRRECAESFPEYKIKPPKETLTLSVPRSSCQQCGTPIRIR
DNIPVISWLLLKGKCHNCQAPISARYPLIELLTAFCSGFIAFHFGFSYFTVALVFFTFVLIAATFIDLDTMLLPDQLTLP
LMWAGIALALAGISPVSLQDSIIGAMAGYLCLWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQSLPMIILLSSVVGV
IFGLIQLRLQKQGIEKAFPFGPYLAIAGWVSLIWGHQILNWYFTSILGV

Nucleotide

• Length: 870 bp

>NTDB_id=788957 PUN50_RS02900 WP_274290832.1 596872..597741(-) (pilD) [Vibrio campbellii strain 20220413_1]
ATGGAAGTATTCCAGTACTACCCATGGCTATTCGTTGTATTTGCCACAATTTTCGGTTTGATTGTGGGTAGTTTCCTCAA
CGTAGTTATCTACCGTTTACCCAAGATCATGGAACTAGAATGGCGACGTGAGTGCGCTGAATCCTTCCCTGAATATAAGA
TTAAACCACCCAAAGAGACGCTGACGTTAAGCGTACCGCGCTCATCTTGTCAGCAATGCGGAACTCCCATTCGCATCCGA
GACAACATTCCGGTGATCAGCTGGCTATTGCTGAAAGGCAAGTGCCACAACTGCCAAGCGCCCATCAGCGCTCGCTACCC
TCTTATTGAACTGCTTACTGCGTTTTGTTCGGGTTTTATCGCTTTTCATTTCGGTTTTAGCTACTTCACTGTAGCGCTGG
TTTTCTTTACCTTTGTATTGATTGCTGCCACGTTTATCGATCTCGATACCATGTTGTTGCCAGACCAACTGACTCTACCA
TTGATGTGGGCAGGTATCGCACTTGCTCTTGCTGGTATCAGTCCTGTCAGCCTACAAGATTCTATCATTGGTGCAATGGC
GGGCTATCTGTGTCTTTGGAGTGTTTACTGGCTGTTTAAACTCCTAACAGGCAAAGAAGGCATGGGCTATGGTGACTTTA
AACTCCTTGCGGCATTGGGCGCTTGGCTGGGTTGGCAATCTCTACCGATGATCATTTTGCTCTCTTCGGTGGTCGGCGTC
ATCTTTGGTCTTATCCAACTGCGCTTGCAAAAACAAGGTATCGAGAAAGCCTTTCCTTTCGGCCCTTACCTTGCGATTGC
TGGTTGGGTGAGCCTAATTTGGGGCCATCAAATCCTCAACTGGTACTTTACGTCGATTCTAGGAGTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	98.27	100	0.983
pilD	Vibrio cholerae strain A1552	75.694	99.654	0.754
pilD	Acinetobacter baumannii D1279779	52.768	93.772	0.495
pilD	Acinetobacter nosocomialis M2	52.399	93.772	0.491
pilD	Neisseria gonorrhoeae MS11	46.014	95.502	0.439