Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PUB92_RS17885 Genome accession   NZ_CP117919
Coordinates   3970278..3971387 (+) Length   369 a.a.
NCBI ID   WP_274181634.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain PSKL.DM     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3965278..3976387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUB92_RS17850 (PUB92_17850) - 3965886..3966197 (+) 312 WP_274181632.1 hypothetical protein -
  PUB92_RS17855 (PUB92_17855) - 3966260..3966679 (-) 420 WP_274181633.1 DUF4426 domain-containing protein -
  PUB92_RS17860 (PUB92_17860) yggU 3966698..3966997 (-) 300 WP_005304998.1 DUF167 family protein YggU -
  PUB92_RS17865 (PUB92_17865) - 3966997..3967548 (-) 552 WP_017780458.1 YggT family protein -
  PUB92_RS17870 (PUB92_17870) proC 3967589..3968413 (-) 825 WP_123246602.1 pyrroline-5-carboxylate reductase -
  PUB92_RS17875 (PUB92_17875) - 3968474..3969175 (-) 702 WP_099359980.1 YggS family pyridoxal phosphate-dependent enzyme -
  PUB92_RS17880 (PUB92_17880) pilT 3969215..3970249 (+) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  PUB92_RS17885 (PUB92_17885) pilU 3970278..3971387 (+) 1110 WP_274181634.1 type IVa pilus ATPase TapU Machinery gene
  PUB92_RS17890 (PUB92_17890) yaaA 3971419..3972192 (+) 774 WP_274181635.1 peroxide stress protein YaaA -
  PUB92_RS17895 (PUB92_17895) srmB 3972369..3973592 (-) 1224 WP_010636177.1 ATP-dependent RNA helicase SrmB -
  PUB92_RS17900 (PUB92_17900) - 3973721..3974431 (+) 711 WP_201971278.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  PUB92_RS17905 (PUB92_17905) brnQ 3974623..3975930 (+) 1308 WP_274181636.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41108.25 Da        Isoelectric Point: 6.5672

>NTDB_id=788354 PUB92_RS17885 WP_274181634.1 3970278..3971387(+) (pilU) [Aeromonas dhakensis strain PSKL.DM]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAAAPLDKARALALVKETLNPEHFERYLRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSPDGRRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGSGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=788354 PUB92_RS17885 WP_274181634.1 3970278..3971387(+) (pilU) [Aeromonas dhakensis strain PSKL.DM]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAATGGCCACCTGCAGTCGCTGGCAGCAGCGCCCCTCGACAAGGCAAGAGCACTGGCGCTGGTGAAGGAGA
CCCTGAACCCGGAGCACTTCGAGCGCTACCTGCGCACCAAGGAGGCCAACTACGCCATCCACCGCGAAGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAACTGCCCGGCATGGTGCTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATATTGCAGGAGATTGCCCTCGCCAAGCGCGGGCTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACTGAGTCGTTCGACGT
GGCGCTGAAGAGCTCGCTGCGCCAGGCCCCTGACGTGATCCTGATCGGCGAAATCCGCAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGTCATCTCTGCCTCGCCACCCTGCATGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTGCACCTGGTGCCCCAGGAGAAACACCGTCAGTTCCTGTTCGATCTCTCCTTCAATCTGCGCGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCCGGATGGACGGCGTCGTTTTGCCGCCTTCGAGGTGTTGCTCAACACCCCGCTCATCACCGATATCA
TCCGCAAGGGGGAGATGCACCGCCTCAAGGAGGTGATGACCAAGTCCACCGAACTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACTTGCGACT
GCTGATCAAGCTGTCGGGCCGAGAACAGCTGGGTTCAGGCACACTCGACAACGTCACCCTGGATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.87

99.729

0.607
  pilU Pseudomonas stutzeri DSM 10701

60.114

95.122

0.572
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.598

89.702

0.382
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Acinetobacter baylyi ADP1

39.589

92.412

0.366