Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSH77_RS04565 Genome accession   NZ_CP117459
Coordinates   1016508..1017716 (-) Length   402 a.a.
NCBI ID   WP_305431136.1    Uniprot ID   -
Organism   Pseudomonas extremorientalis strain FP1754     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1011508..1022716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH77_RS04525 (PSH77_04525) - 1011540..1012838 (-) 1299 WP_071491077.1 NAD(P)/FAD-dependent oxidoreductase -
  PSH77_RS04530 (PSH77_04530) - 1012899..1013072 (-) 174 WP_071491078.1 DUF3094 family protein -
  PSH77_RS04535 (PSH77_04535) - 1013129..1013758 (-) 630 WP_010213242.1 DUF1780 domain-containing protein -
  PSH77_RS04540 (PSH77_04540) - 1013860..1014549 (+) 690 WP_071491079.1 energy-coupling factor ABC transporter permease -
  PSH77_RS04545 (PSH77_04545) - 1014616..1014831 (+) 216 WP_003171680.1 hypothetical protein -
  PSH77_RS04550 (PSH77_04550) yacG 1014828..1015028 (-) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  PSH77_RS04555 (PSH77_04555) coaE 1015025..1015648 (-) 624 WP_071491080.1 dephospho-CoA kinase -
  PSH77_RS04560 (PSH77_04560) pilD 1015645..1016511 (-) 867 WP_071491081.1 A24 family peptidase Machinery gene
  PSH77_RS04565 (PSH77_04565) pilC 1016508..1017716 (-) 1209 WP_305431136.1 type II secretion system F family protein Machinery gene
  PSH77_RS04570 (PSH77_04570) - 1017910..1018332 (+) 423 WP_083351537.1 pilin -
  PSH77_RS04575 (PSH77_04575) - 1018368..1019768 (+) 1401 WP_305431138.1 O-antigen ligase -
  PSH77_RS04585 (PSH77_04585) - 1020034..1020339 (+) 306 WP_033903347.1 DUF6388 family protein -
  PSH77_RS04590 (PSH77_04590) nadC 1020387..1021235 (-) 849 WP_071491085.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43076.36 Da        Isoelectric Point: 9.8382

>NTDB_id=785664 PSH77_RS04565 WP_305431136.1 1016508..1017716(-) (pilC) [Pseudomonas extremorientalis strain FP1754]
MDNASTIYAWEGINRKGRRVSGHSTGHNPALVKAQLRRQGISPGLVRKHSPVLAGLKPSIKSADITQLTRQLATLLKAGI
PLLQALDIISEGFDNATLRELLQGLQQSIAAGTSLADALRKHPRCFDELYCNLVAAGEQAGALETLLERVAIHREKSEQL
KARIKKAMTYPITVLIVASLVTGVLLVHVVPQFQSLFAGVDGKLPGFTLQVIALSEFMQRAWWGVALGLLAAGFGLRHAY
HALPAFQLWLDAGLLKVPVAGKLLRKSAVARYARTLSTTFAAGVPLVQALDSVAGAAGSGPFRQAIEHMRHDVSTGMQLN
QSMAVSGLFPGMAIQMTAIGEESGTLDRMLEKVANQYEADVDTLVDHLTSLMEPLIMVVLGGIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=785664 PSH77_RS04565 WP_305431136.1 1016508..1017716(-) (pilC) [Pseudomonas extremorientalis strain FP1754]
ATGGACAACGCTTCAACGATCTACGCTTGGGAAGGCATCAACCGCAAAGGGCGCAGGGTGTCCGGGCACAGCACCGGGCA
CAACCCTGCCCTGGTCAAGGCTCAGCTGCGCCGACAAGGCATCAGCCCAGGGCTTGTACGCAAGCATTCTCCAGTTCTAG
CGGGCCTCAAGCCATCTATCAAATCAGCGGATATCACCCAGCTCACGCGCCAACTGGCAACCTTGCTGAAGGCCGGGATT
CCTCTGCTGCAAGCCCTCGACATCATCAGCGAAGGCTTTGACAACGCTACGCTGCGCGAGCTGCTACAGGGGTTGCAACA
GTCAATCGCCGCTGGCACCAGCCTTGCCGACGCGTTGCGCAAGCATCCGCGCTGTTTCGATGAGCTGTATTGCAACCTGG
TGGCGGCCGGCGAACAGGCCGGTGCCCTTGAAACCTTGCTGGAGCGCGTAGCGATTCATAGGGAAAAGAGCGAGCAGTTA
AAGGCCAGGATCAAAAAAGCCATGACCTATCCGATCACCGTGCTGATAGTCGCCAGCCTGGTCACCGGCGTGCTGCTGGT
CCACGTAGTGCCGCAGTTCCAAAGCCTGTTCGCCGGAGTCGACGGCAAACTGCCCGGCTTCACCTTGCAGGTGATCGCTT
TGTCCGAGTTCATGCAGCGAGCCTGGTGGGGCGTGGCACTGGGCCTGCTTGCCGCGGGTTTTGGGTTGCGCCACGCCTAC
CATGCCTTACCGGCTTTCCAGCTGTGGCTGGATGCCGGTTTGTTGAAAGTGCCCGTGGCAGGCAAACTGCTGAGAAAATC
GGCCGTGGCCCGCTACGCCCGCACGCTGTCCACTACCTTCGCCGCCGGTGTTCCGTTGGTGCAGGCCCTGGACTCGGTGG
CCGGGGCCGCCGGTTCTGGGCCGTTCAGACAGGCAATCGAGCATATGCGACACGACGTATCCACTGGCATGCAACTGAAT
CAATCCATGGCCGTCAGCGGCCTGTTTCCCGGCATGGCCATCCAGATGACGGCCATCGGCGAAGAGTCGGGCACGCTTGA
CCGCATGCTGGAGAAGGTCGCCAACCAGTACGAGGCCGATGTGGACACCCTGGTCGATCACCTCACTAGCCTGATGGAGC
CTCTGATCATGGTGGTACTGGGGGGCATCGTCGGCGCGCTGGTGATTGCCATGTACCTGCCGGTATTCCAACTGGGGAGC
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(69-191)

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.942

98.756

0.582
  pilC Acinetobacter baylyi ADP1

51.263

98.507

0.505
  pilC Acinetobacter baumannii D1279779

50.886

98.259

0.5
  pilC Legionella pneumophila strain ERS1305867

48.354

98.259

0.475
  pilC Vibrio cholerae strain A1552

42.105

99.254

0.418
  pilG Neisseria gonorrhoeae MS11

41.039

95.771

0.393
  pilC Vibrio campbellii strain DS40M4

39.599

99.254

0.393
  pilG Neisseria meningitidis 44/76-A

40.519

95.771

0.388
  pilC Thermus thermophilus HB27

36.591

99.254

0.363