Detailed information NTG0785663

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PSH77_RS04560
• Genome accession: NZ_CP117459
• Coordinates: 1015645..1016511 (-)
• Length: 288 a.a.
• NCBI ID: WP_071491081.1
• Uniprot ID: A0A1H0UQ65
• Organism: Pseudomonas extremorientalis strain FP1754
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1010645..1021511

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSH77_RS04525 (PSH77_04525)	-	1011540..1012838 (-)	1299	WP_071491077.1	NAD(P)/FAD-dependent oxidoreductase	-
PSH77_RS04530 (PSH77_04530)	-	1012899..1013072 (-)	174	WP_071491078.1	DUF3094 family protein	-
PSH77_RS04535 (PSH77_04535)	-	1013129..1013758 (-)	630	WP_010213242.1	DUF1780 domain-containing protein	-
PSH77_RS04540 (PSH77_04540)	-	1013860..1014549 (+)	690	WP_071491079.1	energy-coupling factor ABC transporter permease	-
PSH77_RS04545 (PSH77_04545)	-	1014616..1014831 (+)	216	WP_003171680.1	hypothetical protein	-
PSH77_RS04550 (PSH77_04550)	yacG	1014828..1015028 (-)	201	WP_003188437.1	DNA gyrase inhibitor YacG	-
PSH77_RS04555 (PSH77_04555)	coaE	1015025..1015648 (-)	624	WP_071491080.1	dephospho-CoA kinase	-
PSH77_RS04560 (PSH77_04560)	pilD	1015645..1016511 (-)	867	WP_071491081.1	A24 family peptidase	Machinery gene
PSH77_RS04565 (PSH77_04565)	pilC	1016508..1017716 (-)	1209	WP_305431136.1	type II secretion system F family protein	Machinery gene
PSH77_RS04570 (PSH77_04570)	-	1017910..1018332 (+)	423	WP_083351537.1	pilin	-
PSH77_RS04575 (PSH77_04575)	-	1018368..1019768 (+)	1401	WP_305431138.1	O-antigen ligase	-
PSH77_RS04585 (PSH77_04585)	-	1020034..1020339 (+)	306	WP_033903347.1	DUF6388 family protein	-
PSH77_RS04590 (PSH77_04590)	nadC	1020387..1021235 (-)	849	WP_071491085.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31767.90 Da
• Isoelectric Point: 7.3655

>NTDB_id=785663 PSH77_RS04560 WP_071491081.1 1015645..1016511(-) (pilD) [Pseudomonas extremorientalis strain FP1754]
MSLLLGEHPWAFVAIASVLGLIVGSFLNVLVWRLPKMLEREWRAQANEVLGLPAEPAGPTYNLMQPNSCCPHCNHPIRPW
ENIPLLSYLFLRGRCAHCQGAISIRYPLTELVCSLISAWVAWHFGVGWQAGMVMVLSWGLLAMSLIDADHQLLPDVLVMP
LLWLGLIVNSFGLLTTLADALWGAVIGYMSLWSVFWLFKLATGKDGMGHGDFKLLALLGAWGGWQILPMTLLMASLVGVF
VGLILMRLRNTLASAPMPFGPYLAIAGWIALLWGGQISDFYLQSVGFR

Nucleotide

• Length: 867 bp

>NTDB_id=785663 PSH77_RS04560 WP_071491081.1 1015645..1016511(-) (pilD) [Pseudomonas extremorientalis strain FP1754]
TTGAGCCTGCTGCTGGGCGAGCATCCCTGGGCGTTTGTTGCGATAGCGTCGGTGCTGGGGCTGATCGTCGGCAGTTTTCT
CAACGTGCTGGTGTGGCGCCTGCCGAAAATGCTCGAGCGGGAATGGCGCGCCCAGGCCAATGAAGTGCTGGGCTTGCCAG
CAGAACCAGCCGGGCCGACCTACAACCTGATGCAGCCCAACTCCTGCTGCCCACACTGCAACCATCCCATACGCCCATGG
GAAAATATCCCGCTGCTCAGCTACCTGTTCCTGCGCGGCCGCTGCGCGCATTGCCAAGGGGCGATCAGCATCCGCTACCC
CCTCACGGAACTGGTGTGCAGCTTGATCTCGGCCTGGGTGGCCTGGCATTTCGGAGTTGGCTGGCAGGCAGGAATGGTGA
TGGTGCTGAGTTGGGGGTTGTTGGCGATGAGCCTGATCGACGCCGACCATCAACTGCTGCCGGATGTGCTGGTAATGCCG
CTGCTGTGGCTGGGACTGATCGTCAACAGTTTCGGCCTGCTGACAACATTGGCCGACGCCCTGTGGGGCGCCGTGATTGG
CTACATGAGCCTGTGGAGCGTGTTCTGGTTGTTCAAGCTGGCCACCGGCAAGGACGGCATGGGCCACGGCGACTTCAAGT
TGCTGGCGCTGCTCGGCGCCTGGGGTGGCTGGCAGATCCTGCCGATGACGCTGTTGATGGCGTCACTGGTCGGGGTATTC
GTCGGCTTGATCCTGATGCGCCTGCGCAACACCCTGGCGTCGGCACCGATGCCATTTGGACCGTATCTGGCGATTGCCGG
CTGGATTGCATTGCTCTGGGGTGGTCAAATAAGCGACTTCTATTTGCAGTCTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-124)

• Type IV leader peptidase family (Peptidase_A24) (134-243)

3D structure

• AlphaFold DB: A0A1H0UQ65

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.33	96.875	0.507
pilD	Vibrio campbellii strain DS40M4	49.286	97.222	0.479
pilD	Neisseria gonorrhoeae MS11	50	93.75	0.469
pilD	Acinetobacter nosocomialis M2	49.084	94.792	0.465
pilD	Acinetobacter baumannii D1279779	49.084	94.792	0.465