Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSH60_RS03850 Genome accession   NZ_CP117456
Coordinates   864495..865703 (-) Length   402 a.a.
NCBI ID   WP_198831958.1    Uniprot ID   -
Organism   Pseudomonas simiae strain FP1885     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 859495..870703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH60_RS03810 (PSH60_03810) - 860413..860586 (-) 174 WP_010213243.1 DUF3094 family protein -
  PSH60_RS03815 (PSH60_03815) - 860641..861270 (-) 630 WP_010213242.1 DUF1780 domain-containing protein -
  PSH60_RS03820 (PSH60_03820) - 861374..861841 (+) 468 WP_010213240.1 hypothetical protein -
  PSH60_RS03825 (PSH60_03825) - 861838..862527 (+) 690 WP_044286632.1 energy-coupling factor ABC transporter permease -
  PSH60_RS03830 (PSH60_03830) - 862603..862818 (+) 216 WP_003171680.1 hypothetical protein -
  PSH60_RS03835 (PSH60_03835) yacG 862815..863015 (-) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  PSH60_RS03840 (PSH60_03840) coaE 863012..863635 (-) 624 WP_010213235.1 dephospho-CoA kinase -
  PSH60_RS03845 (PSH60_03845) pilD 863632..864498 (-) 867 WP_069673361.1 A24 family peptidase Machinery gene
  PSH60_RS03850 (PSH60_03850) pilC 864495..865703 (-) 1209 WP_198831958.1 type II secretion system F family protein Machinery gene
  PSH60_RS03855 (PSH60_03855) - 865897..866319 (+) 423 WP_021491914.1 pilin -
  PSH60_RS03860 (PSH60_03860) - 866355..867755 (+) 1401 WP_010213231.1 O-antigen ligase -
  PSH60_RS03870 (PSH60_03870) - 868022..868327 (+) 306 WP_010213230.1 DUF6388 family protein -
  PSH60_RS03875 (PSH60_03875) nadC 868375..869223 (-) 849 WP_010213228.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43444.82 Da        Isoelectric Point: 9.7277

>NTDB_id=785581 PSH60_RS03850 WP_198831958.1 864495..865703(-) (pilC) [Pseudomonas simiae strain FP1885]
MDNASTLYAWEGINRKGRRVSGQTTGHNPALVKAQLRKQGICPGHVRKKSPELSSLAPSITSTDITLFTRQLATLLKAGI
PLLQAFDIIAEGFDNRALRELVQGLKQAIEAGTSLAEALRKQPRCFDELYCNLVAAGEQAGALETLLERVAIHREKSEQL
KTRIKKAMTYPITVLVVASLVTGVLLVHVVPQFQNLFAGVDGKLPGFTLQVIGLSEFMQKAWWAVALGFLAGGLGLREAY
RRLADFRLWLDSCLLKAPVAGKLLSKSAVARFARTLSTTFAAGVPLLQALDSVAGAAGSGPFRRAIEQMRRDVATGMPLN
QSMLTSGLFPGMAIQMTAIGEESGTLDRMLEKVANHYEADVDNLVDHLTHLMEPLIMVVLGGIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=785581 PSH60_RS03850 WP_198831958.1 864495..865703(-) (pilC) [Pseudomonas simiae strain FP1885]
ATGGACAACGCTTCAACGCTCTACGCCTGGGAAGGCATCAACCGCAAAGGACGCAGGGTGTCCGGGCAAACCACCGGACA
CAACCCTGCCCTGGTGAAGGCACAGTTGCGAAAACAGGGCATCTGCCCAGGGCATGTGCGCAAAAAATCCCCAGAGCTGT
CGAGCCTGGCACCCTCGATCACATCAACCGACATCACCCTGTTCACTCGCCAACTGGCCACCCTGCTGAAGGCCGGTATT
CCGCTGCTGCAGGCCTTCGACATCATCGCTGAGGGCTTCGACAACCGCGCCTTGCGTGAGCTTGTGCAAGGGTTGAAACA
GGCCATCGAAGCCGGCACCAGCCTTGCCGAGGCGCTGCGCAAGCAGCCGCGTTGTTTCGACGAGCTGTACTGCAACCTGG
TGGCCGCCGGCGAACAGGCCGGAGCACTTGAAACCTTGCTGGAGCGCGTGGCGATCCACCGGGAAAAGAGCGAGCAGCTA
AAGACGAGGATCAAAAAAGCCATGACCTATCCGATCACCGTGCTGGTGGTCGCCAGCCTCGTCACCGGCGTGCTGCTGGT
CCACGTCGTGCCACAGTTCCAGAACCTGTTTGCCGGGGTCGACGGCAAGCTGCCGGGTTTCACCCTGCAGGTCATCGGCC
TTTCCGAGTTCATGCAGAAAGCCTGGTGGGCCGTGGCGCTGGGCTTTCTTGCGGGAGGCCTGGGGCTGCGCGAAGCGTAT
CGGCGCTTGGCGGATTTCCGTCTATGGCTGGACAGCTGTTTGTTGAAAGCGCCCGTGGCAGGCAAACTGCTGAGCAAATC
CGCCGTGGCCCGCTTCGCCCGCACACTGTCCACCACGTTTGCCGCCGGCGTTCCGTTACTGCAGGCACTGGATTCGGTGG
CCGGTGCCGCCGGTTCCGGGCCATTCAGACGGGCAATCGAACAGATGCGACGCGATGTAGCGACTGGCATGCCATTGAAC
CAATCCATGCTCACCAGCGGCCTGTTCCCCGGCATGGCGATCCAGATGACAGCCATCGGCGAAGAGTCGGGCACACTGGA
CCGCATGCTGGAAAAGGTCGCCAACCATTACGAGGCCGATGTGGACAACCTGGTCGATCACCTCACCCACCTCATGGAGC
CCCTGATCATGGTGGTCCTGGGAGGCATTGTCGGCGCATTGGTGATCGCCATGTACCTGCCAGTCTTCCAGTTGGGGAGC
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)

Type II secretion system (T2SS), protein F (T2SSF)

(272-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

60.101

98.507

0.592
  pilC Acinetobacter baumannii D1279779

52.911

98.259

0.52
  pilC Acinetobacter baylyi ADP1

51.768

98.507

0.51
  pilC Legionella pneumophila strain ERS1305867

50.505

98.507

0.498
  pilC Vibrio cholerae strain A1552

41.414

98.507

0.408
  pilG Neisseria gonorrhoeae MS11

41.667

95.522

0.398
  pilC Vibrio campbellii strain DS40M4

40.355

98.01

0.396
  pilG Neisseria meningitidis 44/76-A

40.885

95.522

0.391
  pilC Thermus thermophilus HB27

36.341

99.254

0.361