Detailed information NTG0785286

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSH79_RS15200
• Genome accession: NZ_CP117445
• Coordinates: 3413169..3414281 (-)
• Length: 370 a.a.
• NCBI ID: WP_305438122.1
• Uniprot ID: -
• Organism: Pseudomonas sp. FP2196
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3408169..3419281

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSH79_RS15185 (PSH79_15180)	-	3408939..3410330 (+)	1392	WP_305438119.1	GntP family permease	-
PSH79_RS15190 (PSH79_15185)	hbdH	3410338..3411111 (+)	774	WP_187679940.1	3-hydroxybutyrate dehydrogenase	-
PSH79_RS15195 (PSH79_15190)	-	3411147..3413102 (+)	1956	WP_305438121.1	acetoacetate--CoA ligase	-
PSH79_RS15200 (PSH79_15195)	pilU	3413169..3414281 (-)	1113	WP_305438122.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSH79_RS15205 (PSH79_15200)	-	3414412..3416949 (+)	2538	WP_305438124.1	PAS domain-containing protein	-
PSH79_RS15210 (PSH79_15205)	-	3417001..3417276 (+)	276	WP_007912322.1	peptidylprolyl isomerase	-
PSH79_RS15215 (PSH79_15210)	-	3417333..3418268 (-)	936	WP_305438139.1	sugar kinase	-

Sequence

Protein

• Length: 370 a.a.
• Molecular weight: 40893.92 Da
• Isoelectric Point: 6.3220

>NTDB_id=785286 PSH79_RS15200 WP_305438122.1 3413169..3414281(-) (pilU) [Pseudomonas sp. FP2196]
MEIDALLSRLSNLQGSDLFLSTAAPPSVRVDGVLTALGEQPFKPGEIAAMAASLMDAEQRREFDRDLEMNLAISRPGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLETVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSTGHIITIED
PIEYIHRHRLSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFAETGHLVLSTLHANNANQALDRI
INMFAEERRAQLLHALGSHLKAFVSQRLVRTLDGQRRAAVEVMLGTPTVADLIRRNELGELKAIMEKSAELGMQTFDAAL
FALVVEGVISEEEALNHADSVNNLRLRLKLHDDGVPGPPPSASGEWGLVD

Nucleotide

• Length: 1113 bp

>NTDB_id=785286 PSH79_RS15200 WP_305438122.1 3413169..3414281(-) (pilU) [Pseudomonas sp. FP2196]
ATGGAAATCGATGCACTGTTATCTCGTTTGTCCAACTTACAGGGGTCTGACCTGTTCCTGTCTACCGCCGCGCCGCCCAG
TGTAAGAGTAGACGGTGTACTGACGGCGCTCGGCGAACAACCCTTCAAACCCGGCGAAATTGCCGCCATGGCGGCCTCAT
TGATGGACGCCGAGCAGCGCCGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCGATCTCACGACCCGGGATTGGCCGC
TTTCGGGTCAATATCTTCAAGCAGCGCAACGATGTGTCGATCGTCATTCGCAATGTAAAACTCGACATTCCGCGTTTCGA
AGACTTGAAACTGCCGCCGGTGCTCCTCGAAACCGTGATGCTCAAACAGGGATTGATCCTGTTTGTCGGCGCCACCGACT
CGGGCAAGTCGACCTCGCTGGCAGCCTTGATCGATCACCGCAACCGCCACAGCACCGGGCACATCATTACTATCGAAGAC
CCGATCGAGTACATCCATCGACACAGGCTGTCGATCATCAATCAGCGGGAAGTCGGCGTCGACACCCGCAGCTTTCATGC
AGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTCGTGCTGATCGGAGAAATCCGTGACCGCGAAACCATGGAACACG
CCTTGGCATTTGCCGAAACCGGGCATCTGGTGCTTTCCACGTTGCACGCCAACAACGCCAATCAGGCGCTGGATCGGATT
ATCAATATGTTTGCCGAAGAGCGGCGTGCGCAGCTGTTGCATGCGTTGGGGAGCCACCTTAAAGCGTTTGTATCGCAACG
ACTGGTACGCACTCTGGACGGGCAGCGCAGAGCGGCGGTCGAGGTGATGCTGGGTACGCCGACGGTCGCGGATCTGATTC
GCCGTAACGAGTTGGGGGAACTGAAGGCGATCATGGAGAAGTCTGCCGAGCTTGGGATGCAGACGTTTGATGCGGCGCTG
TTTGCGTTGGTCGTGGAAGGTGTCATCAGCGAGGAGGAGGCGCTCAACCATGCCGACTCAGTGAACAACCTGCGTCTGCG
CCTGAAACTGCATGATGATGGGGTGCCTGGGCCGCCGCCATCCGCTTCGGGGGAGTGGGGGTTGGTGGATTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (59-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.98	94.865	0.541
pilU	Acinetobacter baylyi ADP1	53.371	96.216	0.514
pilU	Vibrio cholerae strain A1552	52.571	94.595	0.497
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.528	91.622	0.362
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.24	90	0.362
pilT	Vibrio cholerae strain A1552	40.24	90	0.362
pilT	Legionella pneumophila strain Lp02	39.181	92.432	0.362
pilT	Legionella pneumophila strain ERS1305867	39.181	92.432	0.362
pilT	Pseudomonas aeruginosa PAK	38.953	92.973	0.362