Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSH94_RS03815 Genome accession   NZ_CP117444
Coordinates   860028..861236 (-) Length   402 a.a.
NCBI ID   WP_305436527.1    Uniprot ID   -
Organism   Pseudomonas sp. FP2254     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 855028..866236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH94_RS03780 (PSH94_03780) - 856350..856523 (-) 174 WP_181079635.1 DUF3094 family protein -
  PSH94_RS03785 (PSH94_03785) - 856575..857204 (-) 630 WP_181079636.1 DUF1780 domain-containing protein -
  PSH94_RS03790 (PSH94_03790) - 857378..858067 (+) 690 WP_181079637.1 energy-coupling factor ABC transporter permease -
  PSH94_RS03795 (PSH94_03795) - 858133..858348 (+) 216 WP_003171680.1 hypothetical protein -
  PSH94_RS03800 (PSH94_03800) yacG 858345..858545 (-) 201 WP_181079638.1 DNA gyrase inhibitor YacG -
  PSH94_RS03805 (PSH94_03805) coaE 858542..859168 (-) 627 WP_181079639.1 dephospho-CoA kinase -
  PSH94_RS03810 (PSH94_03810) pilD 859165..860031 (-) 867 WP_181079640.1 A24 family peptidase Machinery gene
  PSH94_RS03815 (PSH94_03815) pilC 860028..861236 (-) 1209 WP_305436527.1 type II secretion system F family protein Machinery gene
  PSH94_RS03820 (PSH94_03820) - 861434..861847 (+) 414 WP_181079661.1 pilin -
  PSH94_RS03825 (PSH94_03825) - 861883..863283 (+) 1401 WP_305436529.1 O-antigen ligase -
  PSH94_RS03835 (PSH94_03835) - 863553..863858 (+) 306 WP_305436531.1 DUF6388 family protein -
  PSH94_RS03840 (PSH94_03840) nadC 863920..864768 (-) 849 WP_181079644.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43231.65 Da        Isoelectric Point: 10.1664

>NTDB_id=785256 PSH94_RS03815 WP_305436527.1 860028..861236(-) (pilC) [Pseudomonas sp. FP2254]
MNNASTTYTWEGTTRKGRRVCGQSTAHSPALVKAQLRLQGIRPSRVRRKALLLPSFAVPINTADIALLTRQLATLLKAGI
ALLQALDLISEGVENRAVRELLQALKQQIAAGTSLADALGRHPRYFDELYCNLVAAGEQAGALETLLERVAVHLEKSEQL
KARIKKAMTYPIAVLAVAGVVTAVLLVQVVPQFQGLFAGVGSQLPDFTLAVIALSEFMRQAWWLVALGLGAGCMGLRQAH
RTSSRFRYWLDGGLLKMPLAGKLLQKSAVARYARTLSTTFAAGVPLVQALDSVAGAVGSRPFKQAIEHMRRDVSTGMQLN
RAMASSGLFPGMAIQMTAIGEESGTLDLMLEKVASHYEAEVDNQVDNLTSLMEPLIMVVLGGIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=785256 PSH94_RS03815 WP_305436527.1 860028..861236(-) (pilC) [Pseudomonas sp. FP2254]
ATGAACAACGCTTCGACGACTTACACCTGGGAAGGCACCACGCGCAAAGGGCGCAGGGTGTGCGGGCAAAGCACCGCGCA
CAGTCCGGCCCTGGTCAAGGCGCAACTGCGCCTGCAAGGCATTCGCCCCAGCCGTGTGCGCAGGAAAGCCCTGCTGCTGC
CAAGTTTCGCGGTGCCGATCAACACCGCCGACATCGCCCTGTTAACCCGTCAACTGGCCACATTGCTCAAGGCTGGTATT
GCCTTGCTGCAAGCCCTCGACCTGATCAGCGAAGGTGTCGAAAACCGGGCCGTGCGTGAACTGCTGCAAGCGCTGAAACA
GCAGATCGCCGCCGGCACCAGCCTGGCTGACGCATTGGGCAGGCATCCGCGGTATTTCGATGAGCTGTACTGCAACCTGG
TAGCGGCCGGCGAACAGGCCGGCGCGCTTGAAACCCTGCTGGAGCGCGTAGCGGTTCATCTGGAAAAAAGCGAGCAGCTC
AAAGCCAGGATCAAGAAAGCCATGACGTATCCCATCGCCGTACTGGCGGTGGCCGGGGTGGTTACCGCTGTGCTGCTGGT
GCAAGTGGTGCCGCAGTTCCAGGGCCTGTTCGCTGGCGTCGGCAGTCAGTTGCCGGACTTTACCTTGGCCGTCATCGCCC
TGTCGGAATTCATGCGACAAGCCTGGTGGCTCGTCGCATTGGGGCTTGGCGCGGGGTGCATGGGGCTACGCCAGGCGCAT
CGAACATCTTCACGCTTTCGCTACTGGCTGGATGGGGGTTTGTTGAAAATGCCGCTGGCAGGCAAACTGCTGCAGAAATC
GGCCGTGGCCCGCTACGCCCGCACGCTCTCAACCACATTCGCGGCGGGGGTTCCGCTGGTGCAGGCCTTGGATTCCGTGG
CCGGCGCCGTCGGTTCCAGGCCATTCAAACAGGCAATCGAACATATGCGTCGCGATGTATCCACAGGCATGCAACTGAAC
CGCGCCATGGCCTCCAGCGGCTTGTTTCCCGGCATGGCGATCCAGATGACCGCCATCGGTGAAGAGTCCGGCACGCTGGA
CTTGATGCTGGAGAAGGTCGCCAGCCACTATGAGGCCGAGGTAGACAATCAGGTCGATAACCTCACCAGTCTGATGGAGC
CGCTGATCATGGTGGTACTGGGAGGCATTGTCGGCGCGCTGGTCATCGCCATGTACTTGCCGGTCTTCCAATTGGGGAGC
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(69-191)

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

59.446

98.756

0.587
  pilC Acinetobacter baylyi ADP1

51.646

98.259

0.507
  pilC Acinetobacter baumannii D1279779

51.515

98.507

0.507
  pilC Legionella pneumophila strain ERS1305867

48.737

98.507

0.48
  pilG Neisseria gonorrhoeae MS11

40.355

98.01

0.396
  pilC Vibrio cholerae strain A1552

40

98.259

0.393
  pilG Neisseria meningitidis 44/76-A

39.594

98.01

0.388
  pilC Vibrio campbellii strain DS40M4

37.722

98.259

0.371
  pilC Thermus thermophilus HB27

37.186

99.005

0.368