Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSH86_RS25975 Genome accession   NZ_CP117440
Coordinates   5847630..5848847 (+) Length   405 a.a.
NCBI ID   WP_109756361.1    Uniprot ID   -
Organism   Pseudomonas sp. FP2300     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5842630..5853847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH86_RS25955 (PSH86_25950) - 5844038..5844334 (+) 297 WP_305485864.1 DUF2845 domain-containing protein -
  PSH86_RS25960 (PSH86_25955) - 5844648..5845001 (-) 354 WP_109756359.1 BON domain-containing protein -
  PSH86_RS25965 (PSH86_25960) - 5845283..5845702 (-) 420 WP_134925863.1 pilin -
  PSH86_RS25970 (PSH86_25965) pilB 5845927..5847627 (+) 1701 WP_109756360.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH86_RS25975 (PSH86_25970) pilC 5847630..5848847 (+) 1218 WP_109756361.1 type II secretion system F family protein Machinery gene
  PSH86_RS25980 (PSH86_25975) pilD 5848849..5849718 (+) 870 WP_109756362.1 A24 family peptidase Machinery gene
  PSH86_RS25985 (PSH86_25980) coaE 5849718..5850341 (+) 624 WP_305485871.1 dephospho-CoA kinase -
  PSH86_RS25990 (PSH86_25985) yacG 5850338..5850544 (+) 207 WP_109756364.1 DNA gyrase inhibitor YacG -
  PSH86_RS25995 (PSH86_25990) - 5850541..5850756 (-) 216 WP_013694234.1 hypothetical protein -
  PSH86_RS26000 (PSH86_25995) - 5850820..5851506 (-) 687 WP_116833474.1 energy-coupling factor ABC transporter permease -
  PSH86_RS26005 (PSH86_26000) - 5851652..5852278 (+) 627 WP_003205682.1 DUF1780 domain-containing protein -
  PSH86_RS26010 (PSH86_26005) - 5852275..5852802 (+) 528 WP_116833475.1 MOSC domain-containing protein -
  PSH86_RS26015 (PSH86_26010) - 5852855..5853028 (+) 174 WP_003185543.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44176.82 Da        Isoelectric Point: 9.8765

>NTDB_id=785164 PSH86_RS25975 WP_109756361.1 5847630..5848847(+) (pilC) [Pseudomonas sp. FP2300]
MAVKAVKTDVYTWEGKDRKGTKMTGELTGQSPALVKAQLRKQGINPQKVRKKSTSIFSKGKRIKPLDIALFTRQMATMLK
AGVPLLQAFDIIGEGFDNANMRKLVDEVKQEVAAGNSFAASLRKCPQYFDDLYCNLVDAGEQAGALDTLLDRVATYKEKS
EALKAKIKKAMTYPAAVVLVAAVVTGILLVKVVPQFESVFSGFGAQLPAFTVMVIGLSEFMQESWWILLGGMVGGFFGVK
YALRRSEAFRDWRDKWLLKLPLIGTLMYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKRAVQRIKQDVSTGM
QLNFSMRTSGIFPNLAIQMTAIGEESGALDDMLDKVASFYEAEVDNLVDNLTSLMEPFIMVVLGVVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=785164 PSH86_RS25975 WP_109756361.1 5847630..5848847(+) (pilC) [Pseudomonas sp. FP2300]
ATGGCGGTCAAAGCAGTCAAAACCGATGTTTACACTTGGGAAGGCAAGGACCGCAAAGGCACCAAAATGACCGGCGAGCT
GACGGGCCAGAGCCCGGCCCTGGTCAAGGCACAGCTGCGTAAACAAGGCATCAACCCGCAGAAGGTACGCAAGAAGTCCA
CCTCGATATTCAGCAAGGGCAAGCGCATCAAGCCATTGGATATCGCCCTCTTCACCCGCCAGATGGCAACGATGCTCAAG
GCGGGCGTGCCGCTGTTACAGGCGTTCGACATCATTGGCGAAGGCTTCGACAACGCCAATATGCGCAAGCTGGTGGATGA
GGTGAAACAGGAAGTCGCCGCCGGCAACAGCTTTGCCGCATCGCTGCGCAAATGCCCGCAGTATTTCGACGACCTGTACT
GCAACCTGGTGGACGCCGGTGAACAGGCCGGCGCCCTGGACACGCTGCTGGACCGGGTCGCAACCTACAAGGAAAAGAGC
GAAGCCCTCAAGGCCAAGATCAAGAAAGCCATGACCTATCCAGCCGCCGTCGTTCTCGTTGCTGCAGTGGTCACGGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAGTTCGAATCGGTATTCTCCGGGTTCGGCGCGCAGCTGCCGGCCTTCACGGTGATGG
TCATCGGCTTGTCAGAGTTCATGCAGGAGTCATGGTGGATACTGCTCGGCGGGATGGTGGGCGGATTCTTTGGCGTGAAA
TACGCACTCAGGCGCTCCGAGGCCTTTCGTGACTGGCGCGATAAATGGCTGCTCAAGCTGCCACTGATCGGCACCCTGAT
GTACAAATCCGCCGTGGCCCGCTACGCCCGCACGCTCTCCACCACCTTCGCCGCCGGCGTGCCGCTGGTGGAAGCCCTGG
ACTCGGTGTCTGGCGCCACCGGCAACGTGGTGTTCAAGCGCGCGGTGCAGCGCATCAAGCAAGACGTCTCGACCGGCATG
CAGTTGAATTTCTCCATGCGTACCTCAGGGATCTTTCCGAACCTGGCGATCCAGATGACCGCCATCGGCGAAGAATCCGG
TGCGCTGGATGACATGCTCGACAAGGTGGCGAGCTTTTATGAGGCCGAGGTGGACAATCTGGTGGACAACCTCACCAGCC
TGATGGAACCCTTCATCATGGTGGTCCTGGGGGTCGTCGTCGGTGGCCTGGTGGTTGCCATGTACTTGCCCATCTTTCAA
CTCGGCTCTGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.062

100

0.751
  pilC Acinetobacter baylyi ADP1

59.102

99.012

0.585
  pilC Acinetobacter baumannii D1279779

57.843

100

0.583
  pilC Legionella pneumophila strain ERS1305867

54.545

97.778

0.533
  pilC Vibrio campbellii strain DS40M4

44.081

98.025

0.432
  pilC Vibrio cholerae strain A1552

43.325

98.025

0.425
  pilG Neisseria gonorrhoeae MS11

42.469

100

0.425
  pilG Neisseria meningitidis 44/76-A

41.337

99.753

0.412
  pilC Thermus thermophilus HB27

37.157

99.012

0.368