Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSH70_RS04015 Genome accession   NZ_CP117438
Coordinates   931991..933199 (-) Length   402 a.a.
NCBI ID   WP_053254340.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FP2327     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 926991..938199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH70_RS03975 (PSH70_03975) - 927885..928058 (-) 174 WP_016976295.1 DUF3094 family protein -
  PSH70_RS03980 (PSH70_03980) - 928148..928777 (-) 630 WP_053254334.1 DUF1780 domain-containing protein -
  PSH70_RS03985 (PSH70_03985) - 928881..929348 (+) 468 WP_069553301.1 FAD/FMN-containing dehydrogenase -
  PSH70_RS03990 (PSH70_03990) - 929345..930034 (+) 690 WP_053254336.1 energy-coupling factor ABC transporter permease -
  PSH70_RS03995 (PSH70_03995) - 930099..930314 (+) 216 WP_003171680.1 hypothetical protein -
  PSH70_RS04000 (PSH70_04000) yacG 930311..930511 (-) 201 WP_053254337.1 DNA gyrase inhibitor YacG -
  PSH70_RS04005 (PSH70_04005) coaE 930508..931131 (-) 624 WP_053254338.1 dephospho-CoA kinase -
  PSH70_RS04010 (PSH70_04010) pilD 931128..931994 (-) 867 WP_305439934.1 A24 family peptidase Machinery gene
  PSH70_RS04015 (PSH70_04015) pilC 931991..933199 (-) 1209 WP_053254340.1 type II secretion system F family protein Machinery gene
  PSH70_RS04020 (PSH70_04020) - 933402..933815 (+) 414 WP_069553305.1 pilin -
  PSH70_RS04030 (PSH70_04030) - 934123..934428 (+) 306 WP_053254342.1 DUF6388 family protein -
  PSH70_RS04035 (PSH70_04035) nadC 934475..935323 (-) 849 WP_069553306.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PSH70_RS04040 (PSH70_04040) - 935567..937741 (+) 2175 WP_301279262.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43485.02 Da        Isoelectric Point: 9.9736

>NTDB_id=785104 PSH70_RS04015 WP_053254340.1 931991..933199(-) (pilC) [Pseudomonas fluorescens strain FP2327]
MNEAATIYAWEGINRKGRRVSGQTAGQNLMLVKVQLRQQGICPGRVRKQTSHLPSFTPPIKPADIALFTRQLATLLRAGI
PLLQAFDIISEGFEHRQMRALIKGLKQEIAAGNNLAAALRKQPRYFDELYCNLIAAGEQAGALETLLERVAVHLEKSQQL
KARIKKAMTYPCAVIVVACLVSAILLIHVVPQFQSLFSGVGGELPGFTLTVIALSEFLQHAWWMLALGLGASIAGLRQAY
RTSPGFRHSLDSGLLKAPLAGTLLRKSAIARYARTLSTTFAAGVPLVQALDSVAGATGNGLFKKAIEQMRHDVATGMQLN
HSMAHSRLFPGMAIQMTAIGEESGTLDQMLEKVAQHFEADVENLVDNLTSLMEPLIMVILGGIVGALVVAMYLPVFQLGA
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=785104 PSH70_RS04015 WP_053254340.1 931991..933199(-) (pilC) [Pseudomonas fluorescens strain FP2327]
ATGAATGAAGCTGCAACGATCTACGCCTGGGAAGGCATCAACCGCAAAGGGCGCAGGGTCTCAGGGCAAACCGCTGGCCA
GAACCTTATGCTGGTCAAGGTGCAACTGCGCCAGCAAGGAATCTGCCCCGGCCGCGTACGCAAACAAACCTCGCACCTGC
CAAGCTTTACACCCCCGATAAAACCCGCAGACATTGCCCTGTTCACCCGCCAGCTGGCGACATTGCTGAGGGCCGGCATA
CCGCTGCTGCAAGCCTTCGACATCATCAGCGAAGGCTTCGAACACCGACAGATGCGCGCGCTGATAAAGGGCTTGAAACA
GGAGATCGCCGCCGGTAACAACCTGGCGGCAGCACTGCGCAAACAACCACGCTACTTCGATGAACTGTATTGCAACCTGA
TTGCCGCGGGCGAACAGGCCGGTGCCCTGGAAACCTTGCTGGAGCGGGTGGCGGTACACCTGGAAAAAAGCCAGCAGCTC
AAGGCGCGGATCAAAAAGGCCATGACTTACCCCTGCGCCGTCATCGTGGTCGCCTGCCTTGTCAGTGCGATCCTGCTGAT
CCACGTGGTGCCGCAGTTTCAGAGCCTGTTCAGCGGGGTCGGCGGCGAGCTGCCCGGCTTTACCTTGACGGTCATTGCCC
TGTCCGAATTCCTTCAACACGCCTGGTGGATGCTCGCGCTGGGGCTGGGCGCCAGCATTGCCGGGTTGCGCCAGGCCTAT
CGGACTTCCCCGGGTTTTCGTCACAGCCTGGATAGCGGTTTGTTGAAGGCGCCCCTGGCAGGCACACTGCTAAGGAAGTC
TGCGATTGCCCGCTACGCCCGCACACTTTCAACGACTTTTGCAGCGGGCGTGCCCCTGGTGCAGGCTTTGGATTCCGTGG
CGGGTGCAACGGGCAATGGGTTGTTCAAAAAGGCGATCGAACAGATGCGTCACGACGTAGCCACAGGCATGCAGTTGAAT
CACTCCATGGCTCATAGCCGCCTGTTCCCCGGCATGGCGATCCAGATGACCGCGATCGGTGAAGAATCGGGCACCCTGGA
TCAGATGCTGGAAAAAGTTGCCCAGCATTTTGAGGCCGACGTGGAGAATCTGGTCGACAACCTCACCAGCCTGATGGAGC
CGCTCATCATGGTGATATTGGGGGGCATTGTCGGTGCATTGGTGGTCGCCATGTACCTGCCGGTCTTCCAATTGGGGGCG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.69

98.756

0.58
  pilC Acinetobacter baylyi ADP1

52.778

98.507

0.52
  pilC Acinetobacter baumannii D1279779

51.392

98.259

0.505
  pilC Legionella pneumophila strain ERS1305867

50.126

98.756

0.495
  pilC Vibrio cholerae strain A1552

41.221

97.761

0.403
  pilG Neisseria gonorrhoeae MS11

41.207

94.776

0.391
  pilG Neisseria meningitidis 44/76-A

40.682

94.776

0.386
  pilC Vibrio campbellii strain DS40M4

39.286

97.512

0.383
  pilC Thermus thermophilus HB27

37.905

99.751

0.378