Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSH81_RS04060 Genome accession   NZ_CP117437
Coordinates   948003..949211 (-) Length   402 a.a.
NCBI ID   WP_305392055.1    Uniprot ID   -
Organism   Pseudomonas sp. FP2335     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 943003..954211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH81_RS04020 (PSH81_04020) - 943040..944338 (-) 1299 WP_192297744.1 NAD(P)/FAD-dependent oxidoreductase -
  PSH81_RS04025 (PSH81_04025) - 944399..944572 (-) 174 WP_064450648.1 DUF3094 family protein -
  PSH81_RS04030 (PSH81_04030) - 944627..945256 (-) 630 WP_003188431.1 DUF1780 domain-containing protein -
  PSH81_RS04035 (PSH81_04035) - 945356..946045 (+) 690 WP_192297840.1 energy-coupling factor ABC transporter permease -
  PSH81_RS04040 (PSH81_04040) - 946111..946326 (+) 216 WP_003171680.1 hypothetical protein -
  PSH81_RS04045 (PSH81_04045) yacG 946323..946523 (-) 201 WP_017737928.1 DNA gyrase inhibitor YacG -
  PSH81_RS04050 (PSH81_04050) coaE 946520..947143 (-) 624 WP_305392053.1 dephospho-CoA kinase -
  PSH81_RS04055 (PSH81_04055) pilD 947140..948006 (-) 867 WP_305392054.1 A24 family peptidase Machinery gene
  PSH81_RS04060 (PSH81_04060) pilC 948003..949211 (-) 1209 WP_305392055.1 type II secretion system F family protein Machinery gene
  PSH81_RS04065 (PSH81_04065) - 949414..949827 (+) 414 WP_230412800.1 pilin -
  PSH81_RS04070 (PSH81_04070) - 949863..951266 (+) 1404 WP_305392056.1 O-antigen ligase -
  PSH81_RS04080 (PSH81_04080) - 951516..951821 (+) 306 WP_226456518.1 DUF6388 family protein -
  PSH81_RS04085 (PSH81_04085) nadC 951884..952732 (-) 849 WP_305392057.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43423.90 Da        Isoelectric Point: 10.4522

>NTDB_id=785080 PSH81_RS04060 WP_305392055.1 948003..949211(-) (pilC) [Pseudomonas sp. FP2335]
MDNASTIYAWEGINRKGHRVSGQTVGHNPALIKAQLRQQGICPERVRQRSRVFPDLAPPIKSADIALFTRQLATLLKAGI
ALLQALDILAEGWENRRMQVLIKSLKQEIAGGSNLATALRKQPRHFDALYCNLIAAGEQAGALETLLERVALHLEKTQQL
KARIKKAMTYPAAVLVVAGVVSTLLLVHVVPQFQTLFAGSGGKLPGFTQVVIALSEFLRNAWWLLALGLGTAVAGLRRAY
RVSPGFRHRLQAGLLKAPLAGTLLKKSAVARYARTLSTTFSAGVPLVQALDSVACAVGNGPFRQAIERLRDDVSTGIQLN
QSMTTSGLFPAMAIQMTVIGEESGTLDRMLEKVANHYEADVDTLVDHLTSLMEPLIMVILGGIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=785080 PSH81_RS04060 WP_305392055.1 948003..949211(-) (pilC) [Pseudomonas sp. FP2335]
ATGGACAACGCGTCAACGATCTACGCCTGGGAAGGCATCAACCGCAAAGGGCACAGGGTGTCCGGGCAAACCGTCGGCCA
CAACCCTGCGCTGATCAAGGCGCAACTGCGCCAACAAGGCATCTGCCCCGAGCGTGTGCGCCAGCGCTCCCGTGTTTTTC
CAGACCTCGCCCCACCGATCAAATCCGCCGACATCGCCCTCTTCACCCGACAGTTGGCGACGCTGCTGAAAGCCGGAATT
GCGCTGCTGCAAGCCCTCGACATCCTCGCAGAGGGCTGGGAAAACCGGCGCATGCAGGTGCTGATCAAAAGCTTGAAACA
GGAGATCGCCGGCGGCAGCAACCTGGCCACGGCACTGCGTAAACAGCCGCGCCATTTCGATGCGTTGTACTGCAACTTGA
TTGCCGCGGGCGAGCAGGCAGGCGCCCTGGAAACCCTGTTGGAACGCGTGGCGCTGCACCTGGAGAAAACCCAGCAGCTC
AAGGCGCGGATCAAAAAAGCCATGACCTACCCAGCGGCTGTGCTGGTGGTCGCCGGCGTAGTCAGCACGTTGCTGCTGGT
GCACGTCGTGCCACAGTTCCAGACCCTGTTCGCCGGCAGCGGCGGCAAATTGCCCGGCTTTACCCAGGTGGTCATTGCCC
TGTCGGAATTCCTGCGCAACGCCTGGTGGCTGCTCGCACTCGGCCTGGGCACGGCAGTGGCCGGGCTGCGCCGGGCCTAT
CGTGTGTCCCCAGGGTTTCGCCACCGGCTGCAAGCCGGTTTGTTGAAAGCGCCCCTGGCAGGCACACTGTTGAAAAAGTC
CGCCGTCGCCCGCTACGCTCGCACACTCTCGACTACGTTCAGTGCAGGCGTCCCCCTGGTGCAGGCATTGGACTCGGTCG
CCTGCGCCGTCGGCAACGGGCCATTCAGACAAGCCATCGAGCGCCTGCGCGACGATGTATCCACAGGTATTCAGTTGAAT
CAATCCATGACCACCAGCGGCCTGTTTCCAGCCATGGCGATCCAGATGACCGTTATCGGCGAAGAGTCCGGCACGTTGGA
CCGCATGCTGGAAAAGGTGGCAAACCACTATGAGGCTGACGTGGACACACTGGTCGACCACCTCACCAGCCTGATGGAAC
CGCTGATCATGGTCATACTGGGAGGGATTGTCGGTGCGCTGGTGATCGCAATGTACCTGCCAGTCTTCCAGTTGGGGTCG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.303

98.507

0.545
  pilC Acinetobacter baylyi ADP1

52.525

98.507

0.517
  pilC Acinetobacter baumannii D1279779

50.127

98.259

0.493
  pilC Legionella pneumophila strain ERS1305867

47.089

98.259

0.463
  pilG Neisseria gonorrhoeae MS11

39.086

98.01

0.383
  pilC Vibrio campbellii strain DS40M4

38.832

98.01

0.381
  pilC Vibrio cholerae strain A1552

38.579

98.01

0.378
  pilG Neisseria meningitidis 44/76-A

38.325

98.01

0.376
  pilC Thermus thermophilus HB27

37.221

100

0.373