Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSH65_RS04775 Genome accession   NZ_CP117431
Coordinates   1009490..1010707 (-) Length   405 a.a.
NCBI ID   WP_305425149.1    Uniprot ID   -
Organism   Pseudomonas sp. FP603     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1004490..1015707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH65_RS04740 (PSH65_04740) - 1004911..1006179 (+) 1269 WP_305425143.1 OprD family porin -
  PSH65_RS04745 (PSH65_04745) - 1006186..1006428 (-) 243 WP_305425144.1 DUF2790 domain-containing protein -
  PSH65_RS04750 (PSH65_04750) - 1006821..1007510 (+) 690 WP_305425145.1 energy-coupling factor ABC transporter permease -
  PSH65_RS04755 (PSH65_04755) - 1007574..1007789 (+) 216 WP_007938959.1 hypothetical protein -
  PSH65_RS04760 (PSH65_04760) yacG 1007799..1007999 (-) 201 WP_305425146.1 DNA gyrase inhibitor YacG -
  PSH65_RS04765 (PSH65_04765) coaE 1007996..1008619 (-) 624 WP_305425147.1 dephospho-CoA kinase -
  PSH65_RS04770 (PSH65_04770) pilD 1008616..1009488 (-) 873 WP_305425148.1 A24 family peptidase Machinery gene
  PSH65_RS04775 (PSH65_04775) pilC 1009490..1010707 (-) 1218 WP_305425149.1 type II secretion system F family protein Machinery gene
  PSH65_RS04780 (PSH65_04780) pilB 1010710..1012407 (-) 1698 WP_305425150.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH65_RS04785 (PSH65_04785) - 1012634..1013059 (+) 426 WP_305425151.1 pilin -
  PSH65_RS04790 (PSH65_04790) - 1013377..1013733 (+) 357 WP_305425152.1 BON domain-containing protein -
  PSH65_RS04795 (PSH65_04795) - 1013908..1014204 (-) 297 WP_305425153.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44113.04 Da        Isoelectric Point: 9.9046

>NTDB_id=784940 PSH65_RS04775 WP_305425149.1 1009490..1010707(-) (pilC) [Pseudomonas sp. FP603]
MAVKAAKISVYAWEGTDKKGTKMTGELTGQNPALIKAQLRKQGINPGKVRKKTASIFSAGKRIKALDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDEVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKAKIKKAMTYPLAVVFVAIIVTGILLVKVVPQFESVFSGFGAELPAFTVMVIGLSEFLQAWWWMVLGMLGALVFGVR
HAFKKSPGFRDRMDTWLLKLPLVGALMYKSAVARYARTLSTTFAAGVPLVEALDSVAGATGNIVFKRAVLRIKQDVSTGM
QLNFSMRTSGIFPNMAIQMTAIGEESGALDDMLDKVASFYEDEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=784940 PSH65_RS04775 WP_305425149.1 1009490..1010707(-) (pilC) [Pseudomonas sp. FP603]
ATGGCGGTCAAAGCAGCGAAAATCAGCGTATACGCCTGGGAAGGCACAGACAAAAAAGGCACAAAAATGACCGGCGAATT
GACCGGCCAAAATCCCGCGCTGATCAAGGCCCAGTTACGCAAGCAAGGGATCAACCCGGGCAAGGTGCGGAAAAAAACCG
CGTCCATTTTCAGTGCCGGCAAACGCATCAAGGCGTTGGACATTGCCCTGTTTACCCGGCAGATGGCGACCATGATGAAA
GCCGGCGTACCGCTGCTGCAATCGTTCGACATTATCGGCGAGGGCTTCGATAACCCGAACATGCGCAAGCTGGTAGACGA
GGTGAAACAGGAAGTCGCGGCCGGCAACAGCTTCGCCGCAGCCCTGCGCAAAAAGCCCCAGTATTTCGATGAGCTGTACT
GCAACCTGGTGGATGCCGGCGAACAGGCCGGCGCCCTCGATACCTTGTTGGAACGAGTGGCGACCTACAAGGAAAAGAGC
GAAAGCCTCAAGGCCAAAATCAAGAAAGCCATGACTTACCCGCTGGCGGTGGTGTTCGTCGCGATCATTGTGACCGGGAT
TCTGCTGGTCAAGGTTGTGCCGCAGTTCGAGTCGGTGTTCTCGGGGTTTGGCGCCGAGCTGCCGGCGTTCACCGTGATGG
TCATCGGCTTGTCGGAGTTCCTGCAGGCCTGGTGGTGGATGGTGCTCGGCATGCTGGGTGCGCTGGTCTTCGGCGTCCGC
CATGCCTTTAAAAAGTCCCCGGGCTTTCGGGACCGGATGGACACCTGGCTGCTGAAACTGCCGTTGGTTGGCGCGCTCAT
GTACAAGTCCGCCGTGGCCCGTTACGCCCGCACCTTGTCGACGACCTTTGCCGCCGGGGTGCCACTGGTCGAAGCCCTGG
ATTCAGTGGCGGGCGCGACTGGCAACATCGTGTTCAAGCGAGCGGTGTTACGCATCAAGCAGGACGTTTCAACTGGCATG
CAGCTGAATTTTTCCATGCGCACTTCCGGCATCTTTCCGAACATGGCAATCCAGATGACGGCCATTGGTGAAGAGTCCGG
TGCGCTGGACGACATGCTCGACAAGGTCGCGAGTTTCTATGAGGACGAGGTGGATAACATGGTCGATAACCTGACCAGTC
TGATGGAGCCTTTCATCATGGTGGTGCTGGGGGTTATCGTCGGCGGCCTGGTGGTTGCGATGTACTTGCCTATCTTCCAA
CTTGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.877

100

0.751
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Acinetobacter baumannii D1279779

58.333

100

0.588
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilG Neisseria gonorrhoeae MS11

43.672

99.506

0.435
  pilG Neisseria meningitidis 44/76-A

43.176

99.506

0.43
  pilC Vibrio campbellii strain DS40M4

41.278

100

0.415
  pilC Vibrio cholerae strain A1552

42.172

97.778

0.412
  pilC Thermus thermophilus HB27

38.806

99.259

0.385