Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PIB32_RS01355 Genome accession   NZ_CP116898
Coordinates   330722..331933 (-) Length   403 a.a.
NCBI ID   WP_308366583.1    Uniprot ID   -
Organism   Microbulbifer sp. RZ01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 325722..336933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB32_RS01320 argJ 325888..327090 (+) 1203 WP_308366576.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  PIB32_RS01325 mutT 327101..327505 (+) 405 WP_308366577.1 8-oxo-dGTP diphosphatase MutT -
  PIB32_RS22580 - 327637..327918 (+) 282 WP_374693714.1 BrnT family toxin -
  PIB32_RS01330 - 327920..328144 (+) 225 WP_308366578.1 BrnA antitoxin family protein -
  PIB32_RS01335 yacG 328339..328548 (-) 210 WP_308366579.1 DNA gyrase inhibitor YacG -
  PIB32_RS01340 - 328597..328824 (-) 228 WP_308366580.1 hypothetical protein -
  PIB32_RS01345 coaE 328925..329536 (-) 612 WP_308366581.1 dephospho-CoA kinase -
  PIB32_RS01350 - 329831..330715 (-) 885 WP_308366582.1 A24 family peptidase -
  PIB32_RS01355 pilC 330722..331933 (-) 1212 WP_308366583.1 type II secretion system F family protein Machinery gene
  PIB32_RS01360 pilB 332147..333859 (-) 1713 WP_308366584.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PIB32_RS01365 - 334517..335002 (+) 486 WP_308366585.1 pilin -
  PIB32_RS01370 tfpZ 335157..335894 (+) 738 WP_308366586.1 TfpX/TfpZ family type IV pilin accessory protein -
  PIB32_RS22585 - 336028..336267 (+) 240 WP_374694163.1 transposase -

Sequence

Protein


Download         Length: 403 a.a.        Molecular weight: 43488.25 Da        Isoelectric Point: 9.2569

>NTDB_id=780953 PIB32_RS01355 WP_308366583.1 330722..331933(-) (pilC) [Microbulbifer sp. RZ01]
MANAAAIAYVYKGVDTKGNKVEGEINGTSPALVKAQLRRQGIIANRVQKKPKPLFGTKKKVTPADIALFTRQMATMMKAG
VPLVQSFEIVADGLDNQGVKELLLRIRDDVASGTAFADALRKHPLYFDDLFCNLVASGEQSGALETMLDRIATYKEKTEA
LKAKIKKAMTYPIAVIVVAIVVTSILLIKVVPQFAETFSSFGADLPAFTQMVVGMSEWMQANWFYALLAVVIGIGGTIEV
KKRNKKVAEFFDRLMLKLPILGIITYNSISARFARTLSTTFAAGVPLIDALKSVAGATGNSLYEEATLKIRDSVATGIPL
NGAMRASGLYPTMLVQMAAIGEESGALDEMLAKAADFYEEAVDNMVDNLTTLLEPMIMAVLGILVGGLMIAMYLPIFQLG
QVV

Nucleotide


Download         Length: 1212 bp        

>NTDB_id=780953 PIB32_RS01355 WP_308366583.1 330722..331933(-) (pilC) [Microbulbifer sp. RZ01]
ATGGCCAATGCCGCTGCAATCGCTTATGTCTACAAAGGCGTGGACACCAAAGGCAATAAGGTCGAGGGAGAGATCAACGG
CACCAGCCCGGCACTGGTAAAGGCACAGCTGCGCCGCCAGGGGATTATTGCCAATCGGGTGCAAAAAAAGCCCAAACCAC
TGTTCGGCACCAAGAAGAAGGTCACGCCGGCGGATATTGCCTTGTTTACCCGCCAGATGGCCACCATGATGAAAGCTGGA
GTGCCGCTGGTACAGAGCTTTGAGATTGTGGCCGACGGGCTGGACAATCAGGGGGTAAAGGAACTGCTCCTGAGAATCCG
CGACGATGTGGCCTCGGGCACGGCCTTTGCCGATGCGCTGCGCAAACACCCACTGTATTTTGACGATCTCTTCTGCAACT
TGGTGGCTTCCGGTGAGCAGTCCGGTGCACTGGAAACCATGCTGGACCGCATCGCCACCTACAAAGAGAAAACCGAGGCC
CTCAAGGCCAAAATCAAGAAGGCCATGACCTACCCAATAGCGGTAATTGTGGTAGCTATAGTGGTCACCTCCATCCTGTT
GATTAAGGTGGTGCCCCAGTTTGCGGAAACTTTTTCCAGCTTCGGTGCCGACCTGCCCGCATTTACCCAGATGGTGGTAG
GTATGTCAGAGTGGATGCAGGCCAACTGGTTCTACGCCCTGCTGGCAGTCGTTATCGGTATTGGCGGTACTATAGAAGTG
AAGAAACGCAATAAGAAGGTGGCTGAATTCTTTGACCGCCTAATGCTGAAACTACCTATCTTGGGAATTATTACCTACAA
CTCGATATCTGCTCGCTTCGCTCGCACCCTCTCCACCACCTTTGCCGCAGGTGTACCGCTGATTGATGCACTGAAGTCCG
TTGCTGGCGCTACCGGCAATAGTCTCTACGAAGAAGCCACCTTAAAAATCCGCGATTCTGTGGCCACCGGCATCCCACTC
AATGGCGCTATGCGCGCTTCCGGTCTCTACCCAACTATGCTAGTACAGATGGCGGCAATCGGCGAGGAATCTGGCGCGCT
AGACGAAATGCTGGCCAAGGCCGCGGACTTCTACGAAGAAGCAGTGGACAATATGGTGGACAATCTTACCACCCTGTTGG
AACCAATGATTATGGCCGTGCTGGGTATTTTGGTCGGCGGTTTGATGATTGCCATGTACCTGCCAATCTTCCAGCTAGGT
CAGGTTGTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(69-192)

Type II secretion system (T2SS), protein F (T2SSF)

(273-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

64.141

98.263

0.63
  pilC Acinetobacter baylyi ADP1

61.461

98.511

0.605
  pilC Acinetobacter baumannii D1279779

61.461

98.511

0.605
  pilC Legionella pneumophila strain ERS1305867

56.576

100

0.566
  pilG Neisseria gonorrhoeae MS11

44.444

98.263

0.437
  pilG Neisseria meningitidis 44/76-A

44.192

98.263

0.434
  pilC Vibrio campbellii strain DS40M4

42.105

99.007

0.417
  pilC Vibrio cholerae strain A1552

41.414

98.263

0.407
  pilC Thermus thermophilus HB27

35.492

100

0.367