Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PIB32_RS01360 Genome accession   NZ_CP116898
Coordinates   332147..333859 (-) Length   570 a.a.
NCBI ID   WP_308366584.1    Uniprot ID   -
Organism   Microbulbifer sp. RZ01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 327147..338859
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB32_RS22580 - 327637..327918 (+) 282 WP_374693714.1 BrnT family toxin -
  PIB32_RS01330 - 327920..328144 (+) 225 WP_308366578.1 BrnA antitoxin family protein -
  PIB32_RS01335 yacG 328339..328548 (-) 210 WP_308366579.1 DNA gyrase inhibitor YacG -
  PIB32_RS01340 - 328597..328824 (-) 228 WP_308366580.1 hypothetical protein -
  PIB32_RS01345 coaE 328925..329536 (-) 612 WP_308366581.1 dephospho-CoA kinase -
  PIB32_RS01350 - 329831..330715 (-) 885 WP_308366582.1 A24 family peptidase -
  PIB32_RS01355 pilC 330722..331933 (-) 1212 WP_308366583.1 type II secretion system F family protein Machinery gene
  PIB32_RS01360 pilB 332147..333859 (-) 1713 WP_308366584.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PIB32_RS01365 - 334517..335002 (+) 486 WP_308366585.1 pilin -
  PIB32_RS01370 tfpZ 335157..335894 (+) 738 WP_308366586.1 TfpX/TfpZ family type IV pilin accessory protein -
  PIB32_RS22585 - 336028..336267 (+) 240 WP_374694163.1 transposase -
  PIB32_RS01375 - 336873..337388 (+) 516 WP_308366588.1 hypothetical protein -
  PIB32_RS01380 - 337390..337638 (+) 249 WP_308366589.1 hypothetical protein -
  PIB32_RS01385 - 337631..337801 (+) 171 WP_308366590.1 hypothetical protein -
  PIB32_RS01390 - 337836..338813 (+) 978 WP_308375608.1 transposase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62047.07 Da        Isoelectric Point: 5.9625

>NTDB_id=780954 PIB32_RS01360 WP_308366584.1 332147..333859(-) (pilB) [Microbulbifer sp. RZ01]
MSVTQLSGLAKRLVADQILDESTAVSASKAAQRENQTFAQHAVEAKLVKSRELAKVASLAFGSPLFDLGAYNFDLIPKGL
IDEKLISKHFALPLYKRGNRLFVAVADPTNLAGLDEINFNTGLNTDAVLVEADKLAKAIESFLSNSDIGGGLAGMDDEEL
DALDVGGGADSEPQDDGDPGGDEAPVVRFVNKVLLDAIRTGASDIHFEPYEKSYRVRLRTDGVLHEVAKPPIQLATRISA
RLKVMSKMDISERRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKLGIDALGYEDDQKKIYMDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGINQVNVHPKVGLDFAEALRSFLRQDPDIVMVGEIR
DLTTAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIATSVSVIIAQRLARRLCSECKKPVTLPPEVLKEEGF
DAVNIPQNEWKLFQPVGCEHCSKGYKGRVGVYEVVRITDGISRIIMEGGNSIQIADRARKEGFNNLRTSALRKVVMGVTS
LEEANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=780954 PIB32_RS01360 WP_308366584.1 332147..333859(-) (pilB) [Microbulbifer sp. RZ01]
ATGAGCGTTACCCAACTCTCCGGCTTGGCCAAACGGCTGGTGGCCGATCAGATTCTCGACGAGTCCACCGCCGTCTCTGC
CTCCAAAGCGGCCCAACGGGAGAACCAGACCTTTGCCCAGCACGCCGTGGAGGCCAAGCTGGTCAAAAGCCGCGAACTGG
CCAAGGTGGCCTCCCTGGCCTTTGGCTCACCACTGTTCGACCTGGGCGCCTATAACTTCGACCTGATCCCCAAGGGCCTG
ATCGACGAAAAGTTGATCAGTAAACACTTTGCCCTACCGCTGTACAAGCGCGGTAACCGTCTGTTTGTGGCGGTGGCGGA
CCCCACCAATCTGGCGGGACTGGACGAGATCAACTTCAATACCGGGCTGAATACCGATGCGGTGCTGGTGGAAGCGGACA
AGCTGGCCAAGGCCATTGAGAGCTTCCTCTCCAATAGCGATATCGGAGGCGGCTTGGCGGGCATGGACGATGAAGAGCTG
GACGCTCTGGATGTAGGCGGTGGAGCGGACTCGGAACCGCAAGACGACGGCGATCCCGGCGGCGACGAAGCACCGGTAGT
GCGTTTCGTCAATAAGGTACTGCTGGATGCCATCCGCACCGGCGCCTCGGATATTCACTTCGAACCCTATGAAAAGAGCT
ATCGGGTACGCCTTCGTACAGACGGTGTACTACACGAAGTGGCCAAGCCCCCAATCCAGTTGGCCACTCGTATTTCCGCA
CGCCTGAAAGTAATGTCCAAGATGGATATCTCCGAACGGCGCGTTCCCCAGGATGGCCGTATCAAGATGAAGCTGTCCAA
GACCAAAGCCATCGACTTCCGGGTTAACAGCCTGCCCACCCTGTGGGGTGAAAAAATCGTACTGCGGATTCTCGACCCCT
CCTCAGCCAAACTGGGTATCGACGCCCTGGGCTATGAAGACGACCAGAAAAAAATCTATATGGATGCCCTGGCCCAGCCC
CAGGGCATGATCCTGGTCACCGGGCCCACCGGTTCGGGCAAGACGGTCTCCCTGTATACAGGCCTGAATATCCTTAATAC
CCCTGAGCGCAATATTTCCACCGCGGAGGACCCGGTGGAAATCAACCTGGAGGGGATTAACCAAGTCAATGTCCACCCCA
AGGTGGGGCTGGACTTCGCCGAGGCGCTGCGCTCTTTCCTGCGCCAGGACCCGGATATCGTGATGGTGGGGGAGATCCGC
GACCTGACAACCGCCGAAATTGCCATCAAGGCCGCGCAAACCGGGCACTTGGTGCTATCCACTCTGCACACCAACTCCGC
CCCGGAAACCCTGACCCGCCTGATGAATATGGGGGTCCCCACCTTCAATATCGCTACGTCGGTGAGTGTGATTATCGCCC
AGCGCCTGGCACGGCGATTGTGTAGCGAGTGCAAAAAGCCTGTTACGCTGCCGCCGGAAGTACTTAAAGAGGAAGGTTTT
GATGCGGTCAATATCCCGCAGAATGAGTGGAAGCTGTTTCAGCCAGTTGGCTGTGAGCACTGCTCCAAGGGTTACAAAGG
CCGCGTGGGTGTGTATGAAGTGGTTCGCATCACTGATGGTATCTCCAGAATTATAATGGAGGGCGGTAATTCCATTCAGA
TTGCCGACCGAGCGCGCAAGGAAGGGTTCAACAACCTGCGCACCTCGGCGTTACGCAAGGTGGTGATGGGTGTAACCAGC
CTGGAGGAGGCGAACCGGGTTACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.62

99.649

0.614
  pilB Acinetobacter baylyi ADP1

60.07

100

0.602
  pilB Legionella pneumophila strain ERS1305867

54.466

100

0.546
  pilB Vibrio parahaemolyticus RIMD 2210633

55.577

91.228

0.507
  pilB Vibrio campbellii strain DS40M4

54.08

92.456

0.5
  pilB Vibrio cholerae strain A1552

52.809

93.684

0.495
  pilF Neisseria gonorrhoeae MS11

48.511

100

0.486
  pilB/pilB1 Synechocystis sp. PCC 6803

41.6

87.719

0.365