Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PH545_RS00025 Genome accession   NZ_CP116414
Coordinates   1767..3506 (+) Length   579 a.a.
NCBI ID   WP_289881545.1    Uniprot ID   -
Organism   Vibrio harveyi strain K2014767     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1..8506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PH545_RS00010 (PH545_00010) pilN 66..641 (+) 576 WP_289880691.1 PilN domain-containing protein Machinery gene
  PH545_RS00015 (PH545_00015) pilO 634..1227 (+) 594 WP_289880692.1 type 4a pilus biogenesis protein PilO Machinery gene
  PH545_RS00020 (PH545_00020) pilP 1217..1732 (+) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  PH545_RS00025 (PH545_00025) pilQ 1767..3506 (+) 1740 WP_289881545.1 type IV pilus secretin PilQ family protein Machinery gene
  PH545_RS00030 (PH545_00030) aroK 3696..4214 (+) 519 WP_289880694.1 shikimate kinase AroK -
  PH545_RS00035 (PH545_00035) aroB 4246..5346 (+) 1101 WP_289880696.1 3-dehydroquinate synthase -
  PH545_RS00040 (PH545_00040) - 5400..6902 (+) 1503 WP_029789966.1 AAA family ATPase -
  PH545_RS00045 (PH545_00045) - 6972..7811 (+) 840 WP_289880698.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 63424.51 Da        Isoelectric Point: 4.5537

>NTDB_id=777265 PH545_RS00025 WP_289881545.1 1767..3506(+) (pilQ) [Vibrio harveyi strain K2014767]
MKMRQGLTLTPRKLTGLFSLWLFLLLAPLVAAEEVKSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYTHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEQEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIQVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGGEDGGGSVGIDDFLNVNLAATSPNATSI
AFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQITP
DNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRRSY
ENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=777265 PH545_RS00025 WP_289881545.1 1767..3506(+) (pilQ) [Vibrio harveyi strain K2014767]
ATGAAAATGAGACAAGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTGTTTCTACTTTTAGC
CCCTTTAGTCGCAGCGGAAGAAGTAAAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACTAATAAAGATAAAGACGCGG
TTATCGTCGTAGAGCTTGCATCGCCAGCTGCTATTGTTGATATCAAGCGTGTGCAAGAAGGTTTAAGTATCGACTTGCTC
AATACGTCAGTTAAAGACGATCAGCTTTATCTACTTGATGTAAAAGACTTCTCTACAGTGGTAGAGAGTGTTGAGGTTTT
CCGCGATACCTCTACGACGCGTTTGGTCGCTTACATTGATGAAGATTATACCCATGACTATCGCCTCACAGGACGTTACC
TTGAGGTGAAGGTTGGTAAGCTCAAAGCCAACGAAAAAGCACCAGACAAAAGTATTCTGGAAAAAGAAGGCAAGTTGATT
TCGATTAACTTCCAAGATATTCCGGTGCGAAATGTGCTGCAGCTTATTGCTGATTACAACCAGTTTAACCTTGTGGTTTC
AGACTCAGTTGAAGGGAACCTGACCTTGCGTTTGGACGGTGTACCTTGGCAACAAGTGCTGGATATCATCCTTCAAGTCA
AAGGCTTGGATAAGCGTGTAGATGGTAACGTTATTTTGGTTGCTCCGAAGGATGAGCTCGATCTGCGCGAGAAACAACAA
CTAGAAAAACAAAAGCTTGAACAAGAAATGGGTGAACTTTCTTCTGAAATTATCAAAGTGAATTTTGCGAAAGCATCGGA
TATTGCAGAGATGATTAACGGTGAAGGTAACATTAGTATGCTTTCTGATCGTGGCAGCATGACGATTGATGAGCGTACTA
ACTCATTATTGATCCGTGAGTTGCCGGAGAATATTCAAGTAATTCGTGAAATCATTGAATCATTAGATATTCCAGTAAAA
CAAGTTCAGATTGAAGCACGTATCGTGACTATCAATGAAGGTAACATGGATGAACTTGGCGTTCGCTGGGGCTTCACATC
GATTAATGGTAGTCATACGGTTGGTGGATCGATCGAGAACAACCTTGCAACTATTGGTCTCTACGATGGCGGCGGTGGTG
AAGACGGTGGCGGCAGTGTTGGTATTGATGACTTCCTGAACGTTAACCTTGCTGCAACCAGCCCGAATGCAACCAGTATT
GCTTTCCAAGTTGCGAAACTAGGGTCTGATACTTTGTTGGATTTGGAGTTGTCTGCGTTGCAACAAGAGTCAAAAGCAGA
AATCATTTCTAGTCCGCGTTTAATTACTACCAATAAGAAGCCAGCTTATATTGAACAAGGTACTGAAATTCCTTACCTAG
AATCCTCTTCCAGCGGTGCGACATCCGTAACGTTCAAAAAAGCGGTGCTGAGCTTAAAAGTGACACCGCAGATCACGCCT
GATAACCGTTTGGTGCTGGATTTGAGTGTTACGCAAGATAGACCGGGTCAAGTGGTAAAAACTGGTACCGGTGAGGCAGT
TGCGATTGATACTCAGCGTATTGGCACTCAAGTATTGGTGAATAATGGTGAGACGGTTGTGCTTGGTGGTATTTTCCAAC
ACAGCATTACAAACTCCGTCGATAAGGTACCTTTATTAGGTGATTTACCGCTGTTAGGGGCACTTTTCCGTCGAAGTTAC
GAAAATGTCGGTAAAAGTGAGTTGCTGATTTTTGTTACGCCTAAAGTGGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(419-577)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

97.586

100

0.978
  pilQ Vibrio cholerae strain A1552

77.522

97.582

0.756
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.522

97.582

0.756
  pilQ Pseudomonas aeruginosa PAK

40.381

99.655

0.402
  pilQ Legionella pneumophila strain Lp02

41.575

94.301

0.392
  pilQ Legionella pneumophila strain ERS1305867

41.575

94.301

0.392
  pilQ Acinetobacter baumannii D1279779

37.186

100

0.383
  pilQ Acinetobacter baumannii strain A118

37.186

100

0.383
  comQ Acinetobacter baylyi ADP1

37.197

99.827

0.371