Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PH545_RS00015 Genome accession   NZ_CP116414
Coordinates   634..1227 (+) Length   197 a.a.
NCBI ID   WP_289880692.1    Uniprot ID   -
Organism   Vibrio harveyi strain K2014767     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1..6227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PH545_RS00010 (PH545_00010) pilN 66..641 (+) 576 WP_289880691.1 PilN domain-containing protein Machinery gene
  PH545_RS00015 (PH545_00015) pilO 634..1227 (+) 594 WP_289880692.1 type 4a pilus biogenesis protein PilO Machinery gene
  PH545_RS00020 (PH545_00020) pilP 1217..1732 (+) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  PH545_RS00025 (PH545_00025) pilQ 1767..3506 (+) 1740 WP_289881545.1 type IV pilus secretin PilQ family protein Machinery gene
  PH545_RS00030 (PH545_00030) aroK 3696..4214 (+) 519 WP_289880694.1 shikimate kinase AroK -
  PH545_RS00035 (PH545_00035) aroB 4246..5346 (+) 1101 WP_289880696.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23100.66 Da        Isoelectric Point: 4.8068

>NTDB_id=777263 PH545_RS00015 WP_289880692.1 634..1227(+) (pilO) [Vibrio harveyi strain K2014767]
MVNWQDLEMDEIAEWLLVPQIVVILLLVVLIQSFGYWFYLKPEQEQLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGDKQSEHFLYRLPLDIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVKANTYQFKPEVKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=777263 PH545_RS00015 WP_289880692.1 634..1227(+) (pilO) [Vibrio harveyi strain K2014767]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATCGCTGAGTGGCTATTGGTGCCTCAGATAGTAGTGATCTTGTTGTT
AGTTGTGCTGATTCAAAGCTTTGGTTATTGGTTTTATTTAAAGCCAGAGCAAGAACAGCTGAATAACCTTATTCAAGAAG
AACAAACCTTAAAAGTTGCGTTACGGATCAAAGCGAACAAAGTCGCTGCTTTGCCTCAATTGCAATCGCAACTTGATGAG
CTTACTACTCGATACGAATTGCTACTGCAACAACTGCCTGCACAGAAAGAGCTGGCAACCATGTTGGCTTCGGTGAATGA
ACTCGGAATTGATAATAAGTTGACGTTCACCCGAATCGACTGGGGTGATAAGCAATCAGAACACTTCCTTTATCGTTTAC
CGCTCGACATTGAGTTGACAGGGGATTTTCATGATATCGGTCGTTTCTCCCAAGCGATTGCGACGTTGCCACGCATCATC
ACTTTTAAAGACGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTCTGCACTTTCGTGTGAAAGCAAACACTTACCA
GTTCAAACCAGAGGTGAAGAAAGATGAGAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

92.632

96.447

0.893
  pilO Vibrio cholerae strain A1552

70.051

100

0.701