Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PJW04_RS14930 Genome accession   NZ_CP116305
Coordinates   3377150..3378280 (-) Length   376 a.a.
NCBI ID   WP_008571827.1    Uniprot ID   A0A3Q9Q1S7
Organism   Xanthomonas perforans strain GEV872     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3372150..3383280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJW04_RS14905 (PJW04_14905) ruvX 3372989..3373456 (-) 468 WP_008571823.1 Holliday junction resolvase RuvX -
  PJW04_RS14910 (PJW04_14910) - 3373449..3374015 (-) 567 WP_003489930.1 YqgE/AlgH family protein -
  PJW04_RS14915 (PJW04_14915) - 3374292..3374864 (+) 573 WP_008571824.1 DNA-3-methyladenine glycosylase I -
  PJW04_RS14920 (PJW04_14920) - 3374872..3375798 (+) 927 WP_031423562.1 DUF72 domain-containing protein -
  PJW04_RS14925 (PJW04_14925) - 3375972..3376646 (-) 675 WP_008571826.1 YitT family protein -
  PJW04_RS14930 (PJW04_14930) pilU 3377150..3378280 (-) 1131 WP_008571827.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PJW04_RS14935 (PJW04_14935) pilT 3378393..3379430 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  PJW04_RS14940 (PJW04_14940) - 3379849..3380541 (+) 693 WP_008571828.1 YggS family pyridoxal phosphate-dependent enzyme -
  PJW04_RS14945 (PJW04_14945) proC 3380585..3381439 (+) 855 WP_033478563.1 pyrroline-5-carboxylate reductase -
  PJW04_RS14950 (PJW04_14950) - 3381999..3382424 (+) 426 WP_008571830.1 HU family DNA-binding protein -
  PJW04_RS14955 (PJW04_14955) - 3382531..3383172 (+) 642 WP_014090668.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42014.24 Da        Isoelectric Point: 6.8286

>NTDB_id=776532 PJW04_RS14930 WP_008571827.1 3377150..3378280(-) (pilU) [Xanthomonas perforans strain GEV872]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=776532 PJW04_RS14930 WP_008571827.1 3377150..3378280(-) (pilU) [Xanthomonas perforans strain GEV872]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCCTCGGACCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGCCCGATTACCCAGACCCCGCTGACTGCGCAGCAGAGCCGCGACCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTATCCGGG
GTCGGGCGTTTCCGTGTGAGCTGCTTCTATCAGCGTAATCAGGTGGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCTTGCCGCCGGTGATCAAGACGCTGTCGATGACCAAGCGCGGCATCATCATCTTTGTCGGCG
CCACCGGAACCGGTAAATCGACTTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAAAATGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCATGCGATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCAGAGGATCGGCGCAACCAGCTATTGATGGACCTGTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGACGGCCGCGGCCGACGCGTGGCGATGGAAATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGATGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAAATCAGTTACGAAGATGCGCTGCGCTACGCCGACTCGCAGAACGA
AGTACGCCTGCGCATCAAGCTCTCGCAGGGTGGCGATGCCAAGACCTTGTCGCAGGGCCTGGACGGTGTGGAAATTTCCG
AAGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q9Q1S7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.03

97.606

0.654
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.737

94.947

0.396
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367