Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PJW04_RS14935 Genome accession   NZ_CP116305
Coordinates   3378393..3379430 (-) Length   345 a.a.
NCBI ID   WP_003489919.1    Uniprot ID   -
Organism   Xanthomonas perforans strain GEV872     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3373393..3384430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJW04_RS14910 (PJW04_14910) - 3373449..3374015 (-) 567 WP_003489930.1 YqgE/AlgH family protein -
  PJW04_RS14915 (PJW04_14915) - 3374292..3374864 (+) 573 WP_008571824.1 DNA-3-methyladenine glycosylase I -
  PJW04_RS14920 (PJW04_14920) - 3374872..3375798 (+) 927 WP_031423562.1 DUF72 domain-containing protein -
  PJW04_RS14925 (PJW04_14925) - 3375972..3376646 (-) 675 WP_008571826.1 YitT family protein -
  PJW04_RS14930 (PJW04_14930) pilU 3377150..3378280 (-) 1131 WP_008571827.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PJW04_RS14935 (PJW04_14935) pilT 3378393..3379430 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  PJW04_RS14940 (PJW04_14940) - 3379849..3380541 (+) 693 WP_008571828.1 YggS family pyridoxal phosphate-dependent enzyme -
  PJW04_RS14945 (PJW04_14945) proC 3380585..3381439 (+) 855 WP_033478563.1 pyrroline-5-carboxylate reductase -
  PJW04_RS14950 (PJW04_14950) - 3381999..3382424 (+) 426 WP_008571830.1 HU family DNA-binding protein -
  PJW04_RS14955 (PJW04_14955) - 3382531..3383172 (+) 642 WP_014090668.1 M23 family metallopeptidase -
  PJW04_RS14960 (PJW04_14960) - 3383230..3383571 (+) 342 WP_003489901.1 DUF3861 family protein -
  PJW04_RS14965 (PJW04_14965) - 3383628..3384170 (+) 543 WP_011348113.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38643.30 Da        Isoelectric Point: 6.6652

>NTDB_id=776533 PJW04_RS14935 WP_003489919.1 3378393..3379430(-) (pilT) [Xanthomonas perforans strain GEV872]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=776533 PJW04_RS14935 WP_003489919.1 3378393..3379430(-) (pilT) [Xanthomonas perforans strain GEV872]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGTCGCATCAATATTCCGGCGTTGGACCACAAGCAGGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGATTACGAGGAATTTCTCGAGGTCGACTTTTCGTTCGAAATTCCGTCGCTGGCGCGT
TTCCGCGTCAATGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCAGGCATGATCGACTACATCAACAAGAATGAGTACGGGCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATTAACCAGCGCGAAGTGCACCGCGATACCCACGGTTTCAACGA
GGCGCTGCGCTCGGCACTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATTCGCCTGG
CGCTCACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCTTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCAGTGATTTCGCAGGC
ACTGCTGAAGAAGGTCGGCGGCGGCCGCACGGCGGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCGCGCGAGTACGCCAAGGACAAGCGGATATTCGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394