Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   PG903_RS13175 Genome accession   NZ_CP115952
Coordinates   2752844..2754109 (+) Length   421 a.a.
NCBI ID   WP_013205094.1    Uniprot ID   -
Organism   Ralstonia solanacearum CFBP2957     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2747844..2759109
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG903_RS13155 ispB 2748302..2749270 (+) 969 WP_013205097.1 octaprenyl diphosphate synthase -
  PG903_RS13165 - 2749547..2750824 (+) 1278 WP_042590559.1 HlyC/CorC family transporter -
  PG903_RS13170 pilB 2751072..2752799 (+) 1728 WP_013205095.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PG903_RS13175 pilG 2752844..2754109 (+) 1266 WP_013205094.1 type II secretion system F family protein Machinery gene
  PG903_RS13180 pilD 2754112..2754993 (+) 882 WP_043892401.1 prepilin peptidase Machinery gene
  PG903_RS13185 coaE 2755006..2755620 (+) 615 WP_013205093.1 dephospho-CoA kinase -
  PG903_RS13190 zapD 2755738..2756496 (+) 759 WP_013205092.1 cell division protein ZapD -
  PG903_RS13195 yacG 2756512..2756730 (+) 219 WP_013205091.1 DNA gyrase inhibitor YacG -
  PG903_RS13200 - 2756723..2757184 (-) 462 WP_042590562.1 NUDIX domain-containing protein -
  PG903_RS13205 - 2757162..2758046 (-) 885 WP_013205089.1 ATP-binding protein -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46511.63 Da        Isoelectric Point: 10.3648

>NTDB_id=774589 PG903_RS13175 WP_013205094.1 2752844..2754109(+) (pilG) [Ralstonia solanacearum CFBP2957]
MATRAPTANRSAAASARKTASTKAPTQYIFEWEGKDRKGKVFKGEMRAENITEVNAVLRKQGLSITKSKRRRAARGKKIT
PKDVAYFTRQLSTMLKAGVPLLQSVDIIARGHANPNFTQLLTEIRVDIESGSSMAQAFRRHPKYFDSLYCNLIDAGEQGG
ILDALLERLSMYMEKSIALKAQIKSAMIYPISVLTVAFLVTVVLMIFVVPAFKNVFASFGATLPAPTLVVMNISDFFVKW
WYIIIFAPAVGIFLYARAFKRSENVQRNTHKAILKMPIFGSIIRKATIARWTRTLATMFSAGTPLVESMESVAGAAGNWV
YHDATLEIQQAVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDNMLLKVAEFYEREVDDAVANISALIEPIIIVILG
VLIGGMVVAMYLPIFKLGQVV

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=774589 PG903_RS13175 WP_013205094.1 2752844..2754109(+) (pilG) [Ralstonia solanacearum CFBP2957]
ATGGCCACACGAGCACCGACCGCGAACCGGTCCGCCGCCGCATCGGCACGCAAAACTGCCTCCACGAAGGCGCCCACGCA
GTACATCTTCGAGTGGGAAGGCAAGGACCGCAAAGGCAAAGTCTTCAAAGGCGAGATGCGGGCGGAGAACATCACCGAAG
TCAACGCGGTGCTGCGCAAGCAGGGGCTGTCGATCACCAAGTCCAAGCGTCGGCGCGCGGCGCGCGGCAAGAAGATCACG
CCCAAGGACGTGGCCTACTTCACGCGCCAGCTGTCGACCATGCTGAAGGCCGGGGTGCCGCTGCTGCAATCGGTCGACAT
CATCGCGCGCGGCCACGCCAACCCGAACTTCACCCAACTGCTGACGGAAATCCGTGTCGACATCGAGTCGGGCAGCAGCA
TGGCGCAGGCCTTCCGCCGCCATCCGAAGTACTTCGATTCGCTGTACTGCAACCTGATCGACGCGGGCGAACAGGGCGGT
ATTCTCGACGCGCTGCTCGAGCGGCTGTCGATGTACATGGAAAAATCGATCGCGCTGAAGGCGCAGATCAAGTCGGCCAT
GATCTACCCAATCTCGGTGCTGACGGTGGCCTTCCTGGTCACGGTGGTGCTGATGATCTTCGTGGTGCCCGCGTTCAAGA
ACGTGTTCGCCAGCTTCGGCGCCACGCTGCCGGCACCGACGCTGGTGGTGATGAATATCTCGGACTTCTTCGTGAAGTGG
TGGTACATCATCATCTTTGCCCCCGCCGTCGGTATCTTCCTGTATGCGCGGGCCTTCAAGCGCTCGGAGAACGTGCAGCG
CAACACGCACAAGGCCATCCTGAAGATGCCGATCTTCGGCAGCATCATCCGCAAGGCCACCATCGCGCGCTGGACGCGCA
CGCTGGCGACGATGTTCTCCGCCGGTACGCCGCTGGTGGAATCGATGGAGTCGGTGGCCGGTGCGGCCGGCAACTGGGTC
TACCACGACGCCACCCTCGAGATCCAGCAGGCGGTACGCATCGGTACCAGCCTCACCAACGCCATGCAGGCCACGCACGT
GTTCGACAACATGGTGCTGCAGATGACGCAGATCGGCGAGGAATCCGGTGCGCTGGACAACATGCTGCTGAAGGTGGCCG
AGTTCTACGAGCGCGAGGTCGATGACGCGGTAGCCAACATCTCCGCCCTGATCGAGCCGATCATCATCGTGATCCTGGGC
GTGCTGATCGGCGGCATGGTGGTCGCCATGTACCTGCCGATCTTCAAGCTGGGCCAGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

50.877

94.774

0.482
  pilG Neisseria meningitidis 44/76-A

50.627

94.774

0.48
  pilC Pseudomonas stutzeri DSM 10701

49.254

95.487

0.47
  pilC Acinetobacter baumannii D1279779

47.42

96.675

0.458
  pilC Acinetobacter baylyi ADP1

46.683

96.675

0.451
  pilC Legionella pneumophila strain ERS1305867

46.02

95.487

0.439
  pilC Vibrio campbellii strain DS40M4

39.012

96.2

0.375
  pilC Vibrio cholerae strain A1552

39

95.012

0.371