Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PG903_RS13170 Genome accession   NZ_CP115952
Coordinates   2751072..2752799 (+) Length   575 a.a.
NCBI ID   WP_013205095.1    Uniprot ID   -
Organism   Ralstonia solanacearum CFBP2957     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2746072..2757799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG903_RS13140 obgE 2746117..2747217 (-) 1101 WP_013205098.1 GTPase ObgE -
  PG903_RS13145 rpmA 2747415..2747675 (-) 261 WP_003271630.1 50S ribosomal protein L27 -
  PG903_RS13150 rplU 2747713..2748024 (-) 312 WP_003271629.1 50S ribosomal protein L21 -
  PG903_RS13155 ispB 2748302..2749270 (+) 969 WP_013205097.1 octaprenyl diphosphate synthase -
  PG903_RS13165 - 2749547..2750824 (+) 1278 WP_042590559.1 HlyC/CorC family transporter -
  PG903_RS13170 pilB 2751072..2752799 (+) 1728 WP_013205095.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PG903_RS13175 pilG 2752844..2754109 (+) 1266 WP_013205094.1 type II secretion system F family protein Machinery gene
  PG903_RS13180 pilD 2754112..2754993 (+) 882 WP_043892401.1 prepilin peptidase Machinery gene
  PG903_RS13185 coaE 2755006..2755620 (+) 615 WP_013205093.1 dephospho-CoA kinase -
  PG903_RS13190 zapD 2755738..2756496 (+) 759 WP_013205092.1 cell division protein ZapD -
  PG903_RS13195 yacG 2756512..2756730 (+) 219 WP_013205091.1 DNA gyrase inhibitor YacG -
  PG903_RS13200 - 2756723..2757184 (-) 462 WP_042590562.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63760.56 Da        Isoelectric Point: 7.1546

>NTDB_id=774588 PG903_RS13170 WP_013205095.1 2751072..2752799(+) (pilB) [Ralstonia solanacearum CFBP2957]
MTLGLALTQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPAVLSANKHF
QQLRLLPLGRRDNRIIVATSDPSDPKPIEELRQKMNVAIETVIVEHDKLMRQLSVANEAPAEIKSLIPKQETTQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKETVERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILTHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=774588 PG903_RS13170 WP_013205095.1 2751072..2752799(+) (pilB) [Ralstonia solanacearum CFBP2957]
ATGACTCTCGGACTCGCACTTACGCAGAGCCGGCGAATTGCGCCGACATTACTCACTCAGCTAGAGCAGAGCGCGCGCGA
AAAGCGCACGCAGTTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCGCACGACCTCGCCGTCTTCGTGGCGGGCA
AGTACCAGATGCCGCTGCTGGACCTGGCGCAGTACAAGCTCGACACCGTGCCGGCCGTGCTGTCGGCCAACAAGCATTTC
CAGCAGTTGCGCCTGCTGCCGCTGGGCCGGCGCGACAACCGCATCATCGTCGCCACCTCGGACCCGAGCGACCCGAAGCC
GATCGAAGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGATGCGCCAGTTGA
GCGTCGCCAACGAAGCGCCGGCCGAGATCAAGTCGCTCATCCCCAAGCAGGAAACCACCCAGATCGAATACGACCCGGGC
GCGGCCGCAGCGGTGCGGCGTACCCCGACCGACGGCATCGACGATGCACCGGTGGTGCGCTTCCTGCAGAAGCTGTTCAC
CGAGGCGCTGCTGCGCGGCACGTCCGACTTGCACTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTTGAACAAGTCGCTCAGCCGCCGCTGGACATCCGCGACAAGATCGCTACCCGTATCAAGGTGTTGTCGCGCCTG
GACATCTCGGAAAAACGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATCACCGTTCCGAAGGACAAGAACGACAAGGA
CAACAAGGAGACGGTCGAGCGCGCTATCGACTTCCGGGTATCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAC
GTGATCAAACGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAGGCTGAACCAGGGCGACATCAACATTTCCACGGCGGAAGATCCGGCGGAAATCCAGCTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAATCGTTCCTGCGTCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGATATCTCCATCAAGGCCGCACAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCGCCGACCACGCTCACACGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCCTCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGGGCCCGCTGCCCAAGGAAACGCTA
CTGGACGCGGGCTTCAAGGAAGCAGACCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCACCCACG
GCACCGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAGGGCGTGCTCTCGCTGCGCGACTCCGGCCTGAGAAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGCTGGCCACGACGAACCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

51.413

98.435

0.506
  pilF Neisseria gonorrhoeae MS11

52.93

94.957

0.503
  pilB Acinetobacter baylyi ADP1

49.735

98.261

0.489
  pilB Legionella pneumophila strain ERS1305867

46.299

100

0.468
  pilB Vibrio cholerae strain A1552

44.643

97.391

0.435
  pilB Vibrio campbellii strain DS40M4

44.891

95.304

0.428
  pilB Vibrio parahaemolyticus RIMD 2210633

44.853

94.609

0.424
  pilB/pilB1 Synechocystis sp. PCC 6803

36.318

100

0.381
  pilF Thermus thermophilus HB27

38.283

97.217

0.372