Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PG878_RS05890 Genome accession   NZ_CP115873
Coordinates   1316339..1318075 (+) Length   578 a.a.
NCBI ID   WP_209029527.1    Uniprot ID   -
Organism   Xanthomonas hawaiiensis strain A6251     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1311339..1323075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG878_RS05860 - 1311498..1311956 (-) 459 WP_311186046.1 chorismate-binding protein -
  PG878_RS05865 (PG878_05855) - 1312025..1312432 (+) 408 WP_209029522.1 RNA-binding S4 domain-containing protein -
  PG878_RS05870 (PG878_05860) coaE 1312575..1313198 (-) 624 WP_209029523.1 dephospho-CoA kinase -
  PG878_RS05875 (PG878_05865) - 1313212..1314075 (-) 864 WP_209029524.1 A24 family peptidase -
  PG878_RS05880 (PG878_05870) pilC 1314082..1315341 (-) 1260 WP_209029525.1 type II secretion system F family protein Machinery gene
  PG878_RS05885 (PG878_05875) comP 1315711..1316136 (+) 426 WP_209029526.1 pilin Machinery gene
  PG878_RS05890 (PG878_05880) pilB 1316339..1318075 (+) 1737 WP_209029527.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PG878_RS05895 (PG878_05885) pilR 1318322..1319710 (-) 1389 WP_209029528.1 sigma-54 dependent transcriptional regulator Regulator
  PG878_RS05900 (PG878_05890) - 1319793..1321406 (-) 1614 WP_209029529.1 ATP-binding protein -
  PG878_RS05905 (PG878_05895) sucC 1321748..1322917 (+) 1170 WP_209029530.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62892.13 Da        Isoelectric Point: 5.8254

>NTDB_id=774110 PG878_RS05890 WP_209029527.1 1316339..1318075(+) (pilB) [Xanthomonas hawaiiensis strain A6251]
MNNLTSANLVGITGIARRLVQDGALEEAAARTAMAHAAEAKVPLPQWFSEKKLVTAAQLAAANAVEFGMPLMDVSVFDAS
QNAMKLVSEELLQKYQVLPLFKRGTRLFVGISNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASSDTFGSALG
GDDEGMDNLDVSTGDEDMGGSGDTGIDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVAKAP
VKLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKNKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPVQLP
EKALLAEGFTEAEIAAGVQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIASAAQKIGVKDLRQSALL
KARNGITSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=774110 PG878_RS05890 WP_209029527.1 1316339..1318075(+) (pilB) [Xanthomonas hawaiiensis strain A6251]
ATGAATAATCTAACATCTGCCAACCTCGTGGGTATCACCGGAATTGCCCGCCGCCTTGTTCAGGATGGCGCGCTGGAGGA
AGCTGCTGCGCGGACGGCGATGGCACACGCGGCCGAGGCCAAGGTCCCGCTGCCGCAGTGGTTCTCCGAGAAGAAGCTGG
TCACTGCCGCGCAGTTGGCCGCCGCGAACGCGGTCGAGTTCGGCATGCCGTTGATGGATGTCTCGGTTTTCGACGCCAGC
CAGAACGCGATGAAGCTGGTCAGCGAAGAACTGCTGCAGAAGTACCAGGTGCTGCCCCTGTTCAAGCGCGGCACCCGCCT
GTTCGTCGGCATCAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCACACCAACCTGACGGTGGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGCCGGACGCTGGAGCAGTGGCAGGCCAGCAGCGACACCTTCGGCAGTGCGCTGGGG
GGCGATGACGAGGGCATGGACAACCTCGACGTCAGCACTGGCGACGAGGACATGGGGGGCAGTGGCGACACTGGCATCGA
CGCCAAGGGCGACGACACCCCGGTCGTCAAGTTCGTGAACAAGGTGCTGGTCGATGCGATCCGCCGCGGCGCGTCCGACA
TCCACTTCGAGCCCTACGAGGACGACTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGAGCGTGGCCAAGGCCCCG
GTCAAGCTCAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGTTGGACATCGCCGAGAAGCGGGTCCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGAACAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACGCTGTTCGGCGAGA
AGATCGTGCTGCGTATCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAACCCGAGCAGCAGAAG
CTGTTCCTGGATGCAATCCACAAGCCGTACGGCATGGTCCTGGTCACCGGACCCACCGGCTCGGGCAAGACGGTGTCGCT
GTACACCGCGCTGGGCATCCTCAACGACGATACCCGCAACATCTCCACCGCCGAGGACCCGGTCGAAATCCGCCTGCCCG
GGGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGT
GCTGTCCACCCTGCATACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCCTACAACATCA
CCAGTTCGGTGACCCTGGTCATCGCCCAGCGCCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCCCGGTCCAGTTGCCC
GAGAAGGCCCTGCTGGCCGAGGGCTTCACCGAGGCGGAGATCGCCGCCGGCGTCCAGCTGTACGAGGCGGTCGGCTGCGA
CGAATGCACCGAGGGCTACAAGGGGCGCACCGGCATCTACCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGC
TGCAGGGCGGCAACGCCATGGACATCGCCAGCGCCGCGCAGAAGATCGGCGTCAAGGACCTGCGCCAGTCAGCCCTGCTC
AAGGCCCGCAACGGCATCACCAGTTTGGCCGAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-152)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.458

98.27

0.545
  pilB Acinetobacter baumannii D1279779

55.166

98.789

0.545
  pilB Legionella pneumophila strain ERS1305867

51.318

98.443

0.505
  pilB Vibrio cholerae strain A1552

49.476

98.962

0.49
  pilF Neisseria gonorrhoeae MS11

49.209

98.443

0.484
  pilB Vibrio campbellii strain DS40M4

45.694

98.443

0.45
  pilB Vibrio parahaemolyticus RIMD 2210633

44.948

99.308

0.446
  pilB/pilB1 Synechocystis sp. PCC 6803

36.23

100

0.382
  pilF Thermus thermophilus HB27

40.192

89.965

0.362