Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PCP56_RS02700 Genome accession   NZ_CP115481
Coordinates   560526..561743 (+) Length   405 a.a.
NCBI ID   WP_003116250.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F002     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 541144..561743 560526..561743 within 0


Gene organization within MGE regions


Location: 541144..561743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP56_RS02555 (PCP56_02555) - 541144..541860 (-) 717 WP_073624880.1 hypothetical protein -
  PCP56_RS02560 (PCP56_02560) - 542009..542209 (+) 201 WP_023435053.1 helix-turn-helix domain-containing protein -
  PCP56_RS02565 (PCP56_02565) - 542206..542622 (+) 417 WP_023435054.1 hypothetical protein -
  PCP56_RS02570 (PCP56_02570) - 542615..542848 (+) 234 WP_023110507.1 hypothetical protein -
  PCP56_RS02575 (PCP56_02575) - 542845..543114 (+) 270 WP_003096138.1 hypothetical protein -
  PCP56_RS02580 (PCP56_02580) - 543111..543416 (+) 306 WP_023109345.1 hypothetical protein -
  PCP56_RS02585 - 543419..543856 (+) 438 WP_305954514.1 hypothetical protein -
  PCP56_RS02590 (PCP56_02590) - 543895..544362 (+) 468 WP_031285502.1 hypothetical protein -
  PCP56_RS02595 (PCP56_02595) - 544369..545322 (+) 954 WP_228294350.1 DUF3631 domain-containing protein -
  PCP56_RS02600 (PCP56_02600) - 545322..545615 (+) 294 WP_023082016.1 hypothetical protein -
  PCP56_RS02605 (PCP56_02605) - 545671..546567 (+) 897 WP_126604972.1 hypothetical protein -
  PCP56_RS02610 (PCP56_02610) - 546698..547111 (+) 414 WP_031629315.1 hypothetical protein -
  PCP56_RS02615 (PCP56_02615) - 547262..547837 (-) 576 WP_124101441.1 hypothetical protein -
  PCP56_RS02620 (PCP56_02620) - 548511..549017 (-) 507 WP_033982511.1 GNAT family N-acetyltransferase -
  PCP56_RS02625 (PCP56_02625) - 549778..550059 (+) 282 WP_033978094.1 hypothetical protein -
  PCP56_RS02630 (PCP56_02630) - 550059..550295 (+) 237 WP_003096116.1 hypothetical protein -
  PCP56_RS02635 (PCP56_02635) - 550502..550783 (-) 282 WP_023435065.1 hypothetical protein -
  PCP56_RS02640 (PCP56_02640) - 550980..551678 (+) 699 WP_124014670.1 hypothetical protein -
  PCP56_RS02645 (PCP56_02645) - 551703..552707 (+) 1005 WP_031689993.1 pesticin C-terminus-like muramidase -
  PCP56_RS02650 (PCP56_02650) - 552720..553382 (+) 663 WP_033992423.1 DUF3218 family protein -
  PCP56_RS02655 (PCP56_02655) fliP 553662..554417 (+) 756 WP_031285507.1 flagellar type III secretion system pore protein FliP -
  PCP56_RS02660 (PCP56_02660) fliQ 554462..554731 (+) 270 WP_023435365.1 flagellar biosynthesis protein FliQ -
  PCP56_RS02665 (PCP56_02665) fliR 554731..555513 (+) 783 Protein_526 flagellar biosynthetic protein FliR -
  PCP56_RS02670 (PCP56_02670) - 555516..555775 (+) 260 Protein_527 EscU/YscU/HrcU family type III secretion system export apparatus switch protein -
  PCP56_RS02675 (PCP56_02675) - 556804..557046 (+) 243 WP_023082027.1 hypothetical protein -
  PCP56_RS02685 (PCP56_02685) - 557289..557987 (-) 699 WP_023122772.1 hypothetical protein -
  PCP56_RS02690 (PCP56_02690) - 558051..558590 (-) 540 WP_003116248.1 pilin -
  PCP56_RS02695 (PCP56_02695) pilB 558819..560522 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCP56_RS02700 (PCP56_02700) pilC 560526..561743 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44051.94 Da        Isoelectric Point: 9.8417

>NTDB_id=772539 PCP56_RS02700 WP_003116250.1 560526..561743(+) (pilC) [Pseudomonas aeruginosa strain F002]
MADKALKTSVFIWEGTDKKGSKVKGELAGQNPMLVKAQLRKQGINPLKVRKKGITLFGAGKKIKPMDIALFTRQMSTMMG
AGVPLLQSFDIIGEGFENPNMRKLVDEIKQDVAAGNSLASSLRKKPIYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVIVVALVVSAILLIKVVPQFQSVFANFGAELPAFTQMVINLSEILQEWWLIVLIGLFAAAFAFR
EVHRRSQKLRDSVDRGLLKLPIIGGILYKSAIARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKNAVTKIKQDVSSGM
QLNFSMRTTNVFPSMAIQMAAIGEESGSLDEMLGKVAGFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=772539 PCP56_RS02700 WP_003116250.1 560526..561743(+) (pilC) [Pseudomonas aeruginosa strain F002]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCATCTGGGAAGGCACTGATAAGAAAGGCAGCAAGGTCAAAGGAGAGTT
GGCCGGGCAAAACCCTATGCTGGTGAAGGCTCAACTGCGCAAACAGGGTATCAACCCACTAAAGGTCCGCAAGAAAGGTA
TCACCCTGTTTGGGGCAGGGAAGAAGATTAAGCCCATGGACATCGCCCTGTTCACTCGGCAGATGTCTACCATGATGGGT
GCCGGCGTACCGTTACTGCAATCTTTTGACATCATCGGCGAAGGATTCGAAAATCCAAACATGCGCAAGCTAGTCGATGA
GATCAAGCAGGATGTTGCCGCCGGTAACAGCTTAGCCAGTTCACTTCGAAAGAAACCCATTTACTTCGATGATCTCTACT
GCAACCTGGTCGATGCTGGCGAACAGTCCGGTGCTTTGGAGACATTATTGGATCGGGTAGCAACTTATAAAGAAAAGACA
GAATCCCTGAAAGCCAAAATTAAAAAGGCCATGACTTATCCCATTGCAGTAATTGTAGTGGCCCTTGTGGTGTCGGCGAT
CCTTCTGATAAAAGTGGTCCCACAGTTCCAGTCCGTATTTGCAAATTTTGGCGCCGAGTTGCCGGCCTTTACTCAAATGG
TCATCAATCTTTCCGAGATTCTTCAAGAGTGGTGGCTCATAGTACTTATTGGTCTTTTTGCCGCAGCTTTTGCATTTAGG
GAAGTTCATCGGCGCTCGCAAAAACTGAGAGATTCAGTAGATCGGGGCCTGCTGAAACTACCTATCATCGGCGGGATACT
TTACAAATCAGCTATCGCCCGCTACGCCCGAACGCTATCCACTACCTTTGCGGCTGGAGTGCCTCTGGTAGAAGCTCTGG
ACTCCGTTTCCGGAGCAACTGGCAACGTGGTATTCAAGAATGCCGTTACCAAGATCAAACAAGACGTGTCCAGCGGCATG
CAATTGAACTTTTCCATGCGCACCACCAATGTTTTCCCCAGTATGGCTATCCAGATGGCTGCAATTGGCGAGGAATCAGG
TTCGCTAGATGAGATGTTAGGAAAAGTTGCCGGTTTCTATGAGGAAGAAGTCGATAACGCCGTCGACAACCTGACAACGT
TGATGGAGCCGATGATCATGGCCGTGCTCGGCGTACTGGTCGGCGGCCTGATCATCGCTATGTATCTACCGATCTTCCAA
CTGGGTTCCGTTGTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.506

100

0.795
  pilC Acinetobacter baumannii D1279779

60.644

99.753

0.605
  pilC Acinetobacter baylyi ADP1

59.606

100

0.598
  pilC Legionella pneumophila strain ERS1305867

55.051

97.778

0.538
  pilG Neisseria meningitidis 44/76-A

46.269

99.259

0.459
  pilG Neisseria gonorrhoeae MS11

46.02

99.259

0.457
  pilC Vibrio cholerae strain A1552

42.222

100

0.422
  pilC Vibrio campbellii strain DS40M4

40.909

97.778

0.4
  pilC Thermus thermophilus HB27

38.404

99.012

0.38