Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PCP56_RS02695 Genome accession   NZ_CP115481
Coordinates   558819..560522 (+) Length   567 a.a.
NCBI ID   WP_003116249.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F002     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 541144..561743 558819..560522 within 0


Gene organization within MGE regions


Location: 541144..561743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP56_RS02555 (PCP56_02555) - 541144..541860 (-) 717 WP_073624880.1 hypothetical protein -
  PCP56_RS02560 (PCP56_02560) - 542009..542209 (+) 201 WP_023435053.1 helix-turn-helix domain-containing protein -
  PCP56_RS02565 (PCP56_02565) - 542206..542622 (+) 417 WP_023435054.1 hypothetical protein -
  PCP56_RS02570 (PCP56_02570) - 542615..542848 (+) 234 WP_023110507.1 hypothetical protein -
  PCP56_RS02575 (PCP56_02575) - 542845..543114 (+) 270 WP_003096138.1 hypothetical protein -
  PCP56_RS02580 (PCP56_02580) - 543111..543416 (+) 306 WP_023109345.1 hypothetical protein -
  PCP56_RS02585 - 543419..543856 (+) 438 WP_305954514.1 hypothetical protein -
  PCP56_RS02590 (PCP56_02590) - 543895..544362 (+) 468 WP_031285502.1 hypothetical protein -
  PCP56_RS02595 (PCP56_02595) - 544369..545322 (+) 954 WP_228294350.1 DUF3631 domain-containing protein -
  PCP56_RS02600 (PCP56_02600) - 545322..545615 (+) 294 WP_023082016.1 hypothetical protein -
  PCP56_RS02605 (PCP56_02605) - 545671..546567 (+) 897 WP_126604972.1 hypothetical protein -
  PCP56_RS02610 (PCP56_02610) - 546698..547111 (+) 414 WP_031629315.1 hypothetical protein -
  PCP56_RS02615 (PCP56_02615) - 547262..547837 (-) 576 WP_124101441.1 hypothetical protein -
  PCP56_RS02620 (PCP56_02620) - 548511..549017 (-) 507 WP_033982511.1 GNAT family N-acetyltransferase -
  PCP56_RS02625 (PCP56_02625) - 549778..550059 (+) 282 WP_033978094.1 hypothetical protein -
  PCP56_RS02630 (PCP56_02630) - 550059..550295 (+) 237 WP_003096116.1 hypothetical protein -
  PCP56_RS02635 (PCP56_02635) - 550502..550783 (-) 282 WP_023435065.1 hypothetical protein -
  PCP56_RS02640 (PCP56_02640) - 550980..551678 (+) 699 WP_124014670.1 hypothetical protein -
  PCP56_RS02645 (PCP56_02645) - 551703..552707 (+) 1005 WP_031689993.1 pesticin C-terminus-like muramidase -
  PCP56_RS02650 (PCP56_02650) - 552720..553382 (+) 663 WP_033992423.1 DUF3218 family protein -
  PCP56_RS02655 (PCP56_02655) fliP 553662..554417 (+) 756 WP_031285507.1 flagellar type III secretion system pore protein FliP -
  PCP56_RS02660 (PCP56_02660) fliQ 554462..554731 (+) 270 WP_023435365.1 flagellar biosynthesis protein FliQ -
  PCP56_RS02665 (PCP56_02665) fliR 554731..555513 (+) 783 Protein_526 flagellar biosynthetic protein FliR -
  PCP56_RS02670 (PCP56_02670) - 555516..555775 (+) 260 Protein_527 EscU/YscU/HrcU family type III secretion system export apparatus switch protein -
  PCP56_RS02675 (PCP56_02675) - 556804..557046 (+) 243 WP_023082027.1 hypothetical protein -
  PCP56_RS02685 (PCP56_02685) - 557289..557987 (-) 699 WP_023122772.1 hypothetical protein -
  PCP56_RS02690 (PCP56_02690) - 558051..558590 (-) 540 WP_003116248.1 pilin -
  PCP56_RS02695 (PCP56_02695) pilB 558819..560522 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCP56_RS02700 (PCP56_02700) pilC 560526..561743 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62610.54 Da        Isoelectric Point: 5.5603

>NTDB_id=772538 PCP56_RS02695 WP_003116249.1 558819..560522(+) (pilB) [Pseudomonas aeruginosa strain F002]
MNDTIQLSGLARQLVLHELLDEKAAQQAQSQAQRNKLSLVTYLVQSKLVKGQALIELAADQFGIAYCDLNSLERDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPKETLLHEGFP
EDRIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=772538 PCP56_RS02695 WP_003116249.1 558819..560522(+) (pilB) [Pseudomonas aeruginosa strain F002]
ATGAACGACACAATTCAACTCAGCGGCTTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAGGCCGCCCAACA
GGCCCAATCGCAGGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAGAGCAAGCTAGTGAAAGGGCAGGCCT
TGATCGAACTGGCTGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGAGCGGGACAGTCTGCCCAAGGAT
CTGATCAGCGAGAAGCTGGTGCGCCAGCATCGAGTAGTTCCGTTGTGGCGACGTGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGACTGGACGACGTGGATCTGGAA
GGCCTGGATGTCGGTTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCGGTGGTGCG
TTTCGTCAACAAAATGTTGTTGGATGCGATCAGAGGCGGCTCCTCCGACCTGCATTTCGAGCCCTATGAAAAGATTTACC
GCGTACGTTTCCGCACCGATGGCATGCTCCATGAAGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTGATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGATTCCTCCA
GCGCGCAGATGGGCATCGACGCCCTCGGCTACGAGGAAGACCAGAAGGAACTCTACCTGGCTGCGCTCAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACTTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGTATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGCCTGCTGAACATGGGCGTGCCAGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGC
GACTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAGCATGAGGTACCGAAGGAAACACTCCTCCATGAAGGCTTCCCC
GAGGATCGGATCGGCACCTTCAAGCTGTATTCACCAGTAGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.895

100

0.582
  pilB Acinetobacter baylyi ADP1

57.27

99.471

0.57
  pilB Legionella pneumophila strain ERS1305867

55.106

100

0.552
  pilF Neisseria gonorrhoeae MS11

50.444

99.295

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

51.948

95.062

0.494
  pilB Vibrio cholerae strain A1552

50.182

97.002

0.487
  pilB Vibrio campbellii strain DS40M4

50.832

95.414

0.485
  pilF Thermus thermophilus HB27

38.584

99.647

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.189

93.474

0.376