Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   H7K60_RS02205 Genome accession   NZ_AP022206
Coordinates   475982..477223 (-) Length   413 a.a.
NCBI ID   WP_011707570.1    Uniprot ID   A0KPV8
Organism   Aeromonas hydrophila strain WP7-S18-ESBL-06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470982..482223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7K60_RS02175 (WP7S18E06_04170) - 471823..473007 (+) 1185 WP_073351955.1 tetratricopeptide repeat protein -
  H7K60_RS02180 (WP7S18E06_04180) mutT 473016..473429 (+) 414 WP_174892455.1 8-oxo-dGTP diphosphatase MutT -
  H7K60_RS02185 (WP7S18E06_04190) yacG 473490..473684 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  H7K60_RS02190 (WP7S18E06_04200) zapD 473694..474416 (-) 723 WP_043160635.1 cell division protein ZapD -
  H7K60_RS02195 (WP7S18E06_04210) coaE 474454..475068 (-) 615 WP_073351951.1 dephospho-CoA kinase -
  H7K60_RS02200 (WP7S18E06_04220) pilD 475087..475959 (-) 873 WP_065475375.1 prepilin peptidase Machinery gene
  H7K60_RS02205 (WP7S18E06_04230) pilC 475982..477223 (-) 1242 WP_011707570.1 type II secretion system F family protein Machinery gene
  H7K60_RS02210 (WP7S18E06_04240) pilB 477339..479045 (-) 1707 WP_073351950.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  H7K60_RS02215 tapA 479049..479492 (-) 444 WP_073352603.1 type IVa pilus major pilin TapA -
  H7K60_RS02220 (WP7S18E06_04250) nadC 479815..480672 (-) 858 WP_073351948.1 carboxylating nicotinate-nucleotide diphosphorylase -
  H7K60_RS02225 (WP7S18E06_04260) - 480678..481148 (-) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  H7K60_RS02230 (WP7S18E06_04270) ampD 481296..481868 (+) 573 WP_073351944.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45422.58 Da        Isoelectric Point: 9.8637

>NTDB_id=77092 H7K60_RS02205 WP_011707570.1 475982..477223(-) (pilC) [Aeromonas hydrophila strain WP7-S18-ESBL-06]
MATLTQKQNAPKKVFAFRWSGVNRKGQKVSGELQADSINTVKAELRKQGVNVTKVSKKSQGLFSKGGAKIKPMDIAVVSR
QITTMLSAGVPLVQSLQIIARSHEKAAMRELMGQIAADVETGTPMSEALRRHPRHFDDLYCDLVEAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPTMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPIFTQFVIGISRFMQNWWYVIFGGIA
LAIFLYVRAWRASQKVKDNTDKFILTIPVVGMILHKAAMARFARTLSTTFSAGIPLVDALVSAAGASGNYVYRTAVMAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVATIFEQEVDDLVDGLTSLLEPLIMVVLGVLVGGMVVA
MYLPIFKLGSVIH

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=77092 H7K60_RS02205 WP_011707570.1 475982..477223(-) (pilC) [Aeromonas hydrophila strain WP7-S18-ESBL-06]
ATGGCAACGCTAACCCAGAAACAGAATGCCCCCAAGAAAGTCTTCGCCTTTCGCTGGAGTGGGGTGAACCGCAAGGGCCA
GAAAGTCTCCGGCGAATTGCAGGCCGACAGCATCAACACGGTCAAGGCAGAGCTGCGCAAACAGGGTGTCAACGTCACCA
AGGTGAGCAAGAAGAGCCAGGGGTTATTCTCCAAGGGCGGCGCCAAGATCAAGCCGATGGACATCGCCGTCGTCTCTCGC
CAGATCACCACCATGCTGTCAGCCGGCGTACCTCTGGTCCAGAGCCTGCAGATCATCGCTCGCAGCCACGAGAAGGCAGC
CATGCGCGAGCTCATGGGGCAGATTGCCGCCGATGTGGAGACCGGCACCCCTATGTCCGAGGCGCTGCGCCGCCATCCCC
GTCATTTTGACGATCTCTACTGCGATCTGGTGGAGGCTGGCGAACAGTCCGGCGCTCTGGAGACCATCTACGATCGCATC
GCCACCTATCGCGAGAAATCGGAAGCCCTCAAGTCCAAGATCAAGAAAGCCATGTTCTACCCCACCATGGTCATCCTGGT
GGCCATTGTTGTTACCTCCATCCTGCTGCTGTTTGTCATTCCCCAGTTCGAGGACATCTTCAAGAGCTTCGGTGCCGAGC
TGCCCATCTTCACCCAATTCGTCATCGGCATCTCCCGTTTCATGCAGAACTGGTGGTATGTGATTTTTGGCGGCATAGCG
CTCGCCATCTTTCTCTACGTGCGCGCCTGGCGCGCCTCCCAGAAGGTCAAGGACAACACCGACAAGTTCATACTGACCAT
CCCGGTGGTTGGCATGATATTGCACAAGGCCGCTATGGCCCGTTTCGCCCGCACCCTCTCCACCACCTTCTCCGCCGGTA
TTCCACTAGTGGATGCCTTGGTCTCTGCGGCAGGTGCCTCCGGTAACTATGTCTACCGCACTGCCGTCATGGCCATTCGC
AACGAGGTGGTGGCCGGCATGCAGATCAACGTGGCCATGCGCACCGTCGATCTGTTCCCAGACATGGTGATCCAGATGGT
GATGATCGGCGAGGAGTCTGGCGCCATCGATGATATGCTCTCCAAGGTCGCCACCATCTTCGAGCAGGAGGTGGACGATC
TGGTCGACGGCCTCACCAGCCTGCTGGAACCTCTCATCATGGTGGTGCTGGGGGTTCTGGTCGGCGGCATGGTGGTCGCC
ATGTATCTGCCCATCTTCAAACTGGGATCGGTGATCCACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0KPV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.323

95.884

0.55
  pilC Acinetobacter baumannii D1279779

54

96.852

0.523
  pilC Legionella pneumophila strain ERS1305867

51.358

98.063

0.504
  pilC Acinetobacter baylyi ADP1

52.141

96.126

0.501
  pilC Vibrio cholerae strain A1552

47.739

96.368

0.46
  pilC Vibrio campbellii strain DS40M4

46.348

96.126

0.446
  pilG Neisseria gonorrhoeae MS11

40.247

98.063

0.395
  pilG Neisseria meningitidis 44/76-A

40

98.063

0.392
  pilC Thermus thermophilus HB27

37.75

96.852

0.366

Multiple sequence alignment