Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   QQX47_RS04375 Genome accession   NZ_CP127122
Coordinates   927651..928115 (+) Length   154 a.a.
NCBI ID   WP_031643283.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain Ned_5_BIM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 922651..933115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QQX47_RS04350 (QQX47_04350) yacG 922821..923021 (-) 201 WP_019486072.1 DNA gyrase inhibitor YacG -
  QQX47_RS04355 (QQX47_04355) coaE 923018..923629 (-) 612 WP_003112838.1 dephospho-CoA kinase -
  QQX47_RS04360 (QQX47_04360) pilD 923626..924498 (-) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  QQX47_RS04365 (QQX47_04365) pilC 924499..925716 (-) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  QQX47_RS04370 (QQX47_04370) pilB 925720..927420 (-) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QQX47_RS04375 (QQX47_04375) pilA/pilAI 927651..928115 (+) 465 WP_031643283.1 pilin Machinery gene
  QQX47_RS04380 (QQX47_04380) - 928273..929586 (+) 1314 WP_408588389.1 O-antigen ligase family protein -
  QQX47_RS04390 (QQX47_04390) nadC 929767..930615 (-) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16049.33 Da        Isoelectric Point: 8.4551

>NTDB_id=770754 QQX47_RS04375 WP_031643283.1 927651..928115(+) (pilA/pilAI) [Pseudomonas aeruginosa strain Ned_5_BIM]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPKNDEYDLGFTS
STLLTGDGKGQIKIDKADTATPEISGTLGGSSGKGIAGAVITVKRDDKGVWTCGITGSPTNWKANYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=770754 QQX47_RS04375 WP_031643283.1 927651..928115(+) (pilA/pilAI) [Pseudomonas aeruginosa strain Ned_5_BIM]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATATCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAACGATTCTCCCAAAAACGATGAGTATGATCTTGGCTTTACCAGT
TCTACTCTGCTTACCGGTGACGGTAAGGGGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
CTTGGGCGGCTCTTCTGGTAAAGGTATTGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAGCCAACTACGCTCCGGCCAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.161

100

0.455
  pilA Pseudomonas aeruginosa PAK

41.772

100

0.429
  pilA Acinetobacter baumannii strain A118

45.07

92.208

0.416
  pilA Ralstonia pseudosolanacearum GMI1000

36.471

100

0.403
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.073

98.052

0.383
  pilA Vibrio cholerae strain A1552

39.073

98.052

0.383
  pilA Vibrio cholerae C6706

39.073

98.052

0.383
  pilA2 Legionella pneumophila str. Paris

37.838

96.104

0.364
  pilA2 Legionella pneumophila strain ERS1305867

37.838

96.104

0.364