Detailed information NTG0770100

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PCP49_RS01330
• Genome accession: NZ_CP115244
• Coordinates: 269674..270822 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain F042
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 264674..275822

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP49_RS01310 (PCP49_01310)	-	265412..266596 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
PCP49_RS01315 (PCP49_01315)	-	266593..267966 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PCP49_RS01320 (PCP49_01320)	-	268209..268613 (-)	405	WP_003084560.1	NINE protein	-
PCP49_RS01325 (PCP49_01325)	czcD	268767..269666 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PCP49_RS01330 (PCP49_01330)	pilU	269674..270822 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PCP49_RS01335 (PCP49_01335)	pilT	271000..272034 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PCP49_RS01340 (PCP49_01340)	-	272249..272941 (+)	693	WP_015649764.1	YggS family pyridoxal phosphate-dependent enzyme	-
PCP49_RS01345 (PCP49_01345)	proC	272953..273774 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PCP49_RS01350 (PCP49_01350)	-	273785..274378 (+)	594	WP_003084544.1	YggT family protein	-
PCP49_RS01355 (PCP49_01355)	-	274375..274671 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=770100 PCP49_RS01330 WP_003100959.1 269674..270822(-) (pilU) [Pseudomonas aeruginosa strain F042]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=770100 PCP49_RS01330 WP_003100959.1 269674..270822(-) (pilU) [Pseudomonas aeruginosa strain F042]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382