Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PCP65_RS04525 Genome accession   NZ_CP115203
Coordinates   986983..988200 (-) Length   405 a.a.
NCBI ID   WP_003161763.1    Uniprot ID   A0A3P3S6C0
Organism   Pseudomonas aeruginosa strain F070     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 981983..993200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP65_RS04480 (PCP65_04485) - 982167..982616 (-) 450 WP_003094670.1 MOSC domain-containing protein -
  PCP65_RS04485 (PCP65_04490) - 982613..983242 (-) 630 WP_031805640.1 DUF1780 domain-containing protein -
  PCP65_RS04490 (PCP65_04495) - 983375..983800 (+) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  PCP65_RS04495 (PCP65_04500) - 983797..984267 (+) 471 WP_003094664.1 hypothetical protein -
  PCP65_RS04500 (PCP65_04505) - 984264..984953 (+) 690 WP_031805639.1 energy-coupling factor ABC transporter permease -
  PCP65_RS04505 (PCP65_04510) - 985059..985268 (+) 210 WP_003094660.1 hypothetical protein -
  PCP65_RS04510 (PCP65_04515) yacG 985305..985505 (-) 201 WP_019486072.1 DNA gyrase inhibitor YacG -
  PCP65_RS04515 (PCP65_04520) coaE 985502..986113 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  PCP65_RS04520 (PCP65_04525) pilD 986110..986982 (-) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PCP65_RS04525 (PCP65_04530) pilC 986983..988200 (-) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  PCP65_RS04530 (PCP65_04535) pilB 988204..989904 (-) 1701 WP_030048379.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCP65_RS04535 (PCP65_04540) - 990135..990602 (+) 468 WP_003161762.1 pilin -
  PCP65_RS04540 (PCP65_04545) - 990760..992073 (+) 1314 WP_223685167.1 O-antigen ligase family protein -
  PCP65_RS04550 (PCP65_04555) nadC 992254..993102 (-) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43998.80 Da        Isoelectric Point: 9.8103

>NTDB_id=769154 PCP65_RS04525 WP_003161763.1 986983..988200(-) (pilC) [Pseudomonas aeruginosa strain F070]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPMDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVAGGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTQMVINISNVLQEWWLLVLLMMGGAGFLLN
HAYKRSEKFRDATDRTVLKLPIVGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVGKIKQDVSTGM
QLNFSMRTTNIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=769154 PCP65_RS04525 WP_003161763.1 986983..988200(-) (pilC) [Pseudomonas aeruginosa strain F070]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAAAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCA
TCTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCTATGGACATCGCCCTGTTCACCCGCCAGATGTCCACCATGATGGCC
GCCGGCGTGCCACTGCTGCAGTCGTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTCGCCGGCGGCAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACT
GCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCAATCGCGGTGATCGTCGTCGCCATCATCGTTTCGGCGAT
CCTACTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTCGAGGGCTTCGGCGCCGAACTGCCGGCCTTCACCCAAATGG
TCATCAATATCTCCAACGTGCTCCAGGAATGGTGGCTCCTGGTACTGCTGATGATGGGCGGCGCCGGCTTCCTGCTGAAC
CACGCCTACAAGCGTTCGGAGAAGTTCCGCGACGCCACCGACCGCACGGTACTGAAGCTGCCGATCGTCGGCGCCATCCT
CTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGTCGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGGGACGCGGTGGGCAAGATCAAGCAGGACGTTTCCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCAACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAATCGGG
CGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAG
CTGGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3P3S6C0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.765

100

0.788
  pilC Acinetobacter baylyi ADP1

61.275

100

0.617
  pilC Acinetobacter baumannii D1279779

60.644

99.753

0.605
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilG Neisseria gonorrhoeae MS11

46.287

99.753

0.462
  pilG Neisseria meningitidis 44/76-A

45.771

99.259

0.454
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Vibrio campbellii strain DS40M4

40.898

99.012

0.405
  pilC Thermus thermophilus HB27

38.155

99.012

0.378