Detailed information NTG0769153

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PCP65_RS04520
• Genome accession: NZ_CP115203
• Coordinates: 986110..986982 (-)
• Length: 290 a.a.
• NCBI ID: WP_023084740.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain F070
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 981110..991982

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP65_RS04475 (PCP65_04480)	-	981971..982141 (-)	171	WP_003094672.1	DUF3094 family protein	-
PCP65_RS04480 (PCP65_04485)	-	982167..982616 (-)	450	WP_003094670.1	MOSC domain-containing protein	-
PCP65_RS04485 (PCP65_04490)	-	982613..983242 (-)	630	WP_031805640.1	DUF1780 domain-containing protein	-
PCP65_RS04490 (PCP65_04495)	-	983375..983800 (+)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
PCP65_RS04495 (PCP65_04500)	-	983797..984267 (+)	471	WP_003094664.1	hypothetical protein	-
PCP65_RS04500 (PCP65_04505)	-	984264..984953 (+)	690	WP_031805639.1	energy-coupling factor ABC transporter permease	-
PCP65_RS04505 (PCP65_04510)	-	985059..985268 (+)	210	WP_003094660.1	hypothetical protein	-
PCP65_RS04510 (PCP65_04515)	yacG	985305..985505 (-)	201	WP_019486072.1	DNA gyrase inhibitor YacG	-
PCP65_RS04515 (PCP65_04520)	coaE	985502..986113 (-)	612	WP_003094654.1	dephospho-CoA kinase	-
PCP65_RS04520 (PCP65_04525)	pilD	986110..986982 (-)	873	WP_023084740.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
PCP65_RS04525 (PCP65_04530)	pilC	986983..988200 (-)	1218	WP_003161763.1	type II secretion system F family protein	Machinery gene
PCP65_RS04530 (PCP65_04535)	pilB	988204..989904 (-)	1701	WP_030048379.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PCP65_RS04535 (PCP65_04540)	-	990135..990602 (+)	468	WP_003161762.1	pilin	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31843.88 Da
• Isoelectric Point: 7.7068

>NTDB_id=769153 PCP65_RS04520 WP_023084740.1 986110..986982(-) (pilD) [Pseudomonas aeruginosa strain F070]
MTLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=769153 PCP65_RS04520 WP_023084740.1 986110..986982(-) (pilD) [Pseudomonas aeruginosa strain F070]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCCCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGTCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGAGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.714	96.552	0.538
pilD	Vibrio campbellii strain DS40M4	53.004	97.586	0.517
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	49.281	95.862	0.472