Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O2T11_RS13090 Genome accession   NZ_CP114186
Coordinates   2756236..2757924 (+) Length   562 a.a.
NCBI ID   WP_283658206.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain PH1273     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2751236..2762924
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O2T11_RS13070 (O2T11_13070) pdhR 2752799..2753566 (-) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  O2T11_RS13075 (O2T11_13075) ampD 2753972..2754523 (-) 552 WP_005462574.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  O2T11_RS13080 (O2T11_13080) nadC 2754616..2755503 (+) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  O2T11_RS13085 (O2T11_13085) pilA 2755768..2756232 (+) 465 WP_031857216.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  O2T11_RS13090 (O2T11_13090) pilB 2756236..2757924 (+) 1689 WP_283658206.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O2T11_RS13095 (O2T11_13095) pilC 2757949..2759172 (+) 1224 WP_064490250.1 type II secretion system F family protein Machinery gene
  O2T11_RS13100 (O2T11_13100) pilD 2759237..2760106 (+) 870 WP_025622886.1 A24 family peptidase Machinery gene
  O2T11_RS13105 (O2T11_13105) coaE 2760107..2760721 (+) 615 WP_025633754.1 dephospho-CoA kinase -
  O2T11_RS13110 (O2T11_13110) zapD 2760749..2761489 (+) 741 WP_023624252.1 cell division protein ZapD -
  O2T11_RS13115 (O2T11_13115) yacG 2761558..2761752 (+) 195 WP_005462546.1 DNA gyrase inhibitor YacG -
  O2T11_RS13120 (O2T11_13120) rplS 2762105..2762458 (-) 354 WP_005462554.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62452.40 Da        Isoelectric Point: 5.4089

>NTDB_id=764922 O2T11_RS13090 WP_283658206.1 2756236..2757924(+) (pilB) [Vibrio parahaemolyticus strain PH1273]
MLLTNLSTVLRQKGLLTFSQEESLIEQVKASGISMPEALLSSGFFTSSELAEHLSSIFGLSQPELSQYEYASLCQQLGLR
ELITRHNALPLHRTPSTLLLAVADPTNQQAEDDFRFATGLQVELVLADFRELSAAIRRLYGRALGKERSQIKEINQEELA
SLVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPNHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLEALRRPQGM
ILMTGPTGSGKTVSLYTGLNILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLD
TAEIAIKASQTGHLVLSTLHTNSAAETIIRLSNMGVESFNLASSLSLIIAQRLARKLCPYCKQPQEHTVQLQHLGIQTTD
NIFIANPDGCNECTHGYSGRTGIYEVMRFDESLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=764922 O2T11_RS13090 WP_283658206.1 2756236..2757924(+) (pilB) [Vibrio parahaemolyticus strain PH1273]
ATGCTACTCACCAACCTCTCTACGGTTCTTCGTCAAAAAGGATTACTGACGTTTTCCCAAGAAGAATCGTTAATAGAGCA
AGTTAAAGCTTCTGGCATCTCGATGCCGGAAGCTTTACTCAGTTCTGGATTCTTCACATCAAGCGAACTGGCCGAGCACT
TAAGTTCTATTTTTGGCCTAAGTCAGCCCGAGTTATCTCAATATGAATATGCTTCGCTTTGCCAACAACTCGGCCTACGT
GAATTAATCACACGACATAATGCACTCCCGCTCCACCGCACGCCTTCAACGTTATTATTAGCGGTTGCCGACCCCACCAA
TCAACAAGCTGAAGATGATTTCCGCTTTGCCACTGGTTTACAGGTTGAATTGGTGTTGGCGGATTTTCGCGAACTCAGTG
CAGCAATACGCCGTCTTTATGGTCGCGCGCTTGGTAAAGAACGATCCCAGATCAAAGAGATCAACCAAGAAGAGCTAGCG
AGTCTGGTGGATGTCGGTGCAGATGAAATCGACAACATCGAAGACTTGAGCCAAGACGAGTCGCCCGTTAGCCGCTACAT
CAACCAAATTCTGTTGGACGCTGTACGCAAAGGCGCATCCGATATCCACTTTGAACCTTATGAAAAGATGTACCGAGTTC
GTCTACGTTGCGATGGCATTTTGATCGAAACTCAGCAACCGCCAAATCACTTGAGTCGTCGCTTATCAGCTCGTATAAAA
ATTCTCTCTAAACTTGATATTGCCGAGCGACGTTTACCACAAGATGGGCGAATTAAACTCAAACTGAATCAAGACACCGC
GATTGATATGCGGGTATCCACTCTACCTACCTTGTTTGGAGAGAAGATCGTACTACGACTGCTCGATAGCAGCTCTGCAT
CACTGGATATCGACAAGCTTGGTTATAGTGAGCAACAAAAACAGCTGTATTTAGAGGCTCTGCGTCGTCCACAAGGTATG
ATTCTGATGACAGGTCCAACAGGAAGCGGCAAAACCGTTTCGTTGTACACTGGGTTAAATATTCTCAATAAACCAGAGAT
CAACATCTCCACAGCGGAAGACCCGGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTCCAACCTAAGATTGGTT
TTGGCTTTGCAGAAGCTCTACGTTCTTTTCTGCGTCAAGATCCCGATGTGGTCATGGTGGGGGAGATTAGAGACCTAGAT
ACTGCGGAAATCGCGATTAAGGCGTCCCAAACCGGTCACTTAGTACTCTCTACTTTGCATACCAACTCTGCGGCTGAAAC
CATCATTCGTTTATCAAACATGGGCGTAGAAAGTTTTAATCTCGCCTCTTCTCTTAGTTTGATTATTGCCCAACGCCTAG
CTCGCAAACTTTGTCCATATTGTAAACAACCGCAAGAGCATACCGTTCAACTTCAGCACCTCGGCATTCAAACAACTGAC
AACATTTTTATAGCCAATCCAGATGGTTGCAACGAATGCACCCATGGTTATTCTGGCCGAACGGGTATCTATGAAGTCAT
GCGCTTTGATGAATCTCTATCCGAAGCTCTTATTAAAGGAGCCTCGGTACATGAACTGGAAAAGCTTGCCATTGCGAATG
GCATGAGTACCTTGCAAATGTCCGGAATAGAAAAACTCAAGCAAGGCATCACAAGCTTTAGTGAGTTACAACGCGTGCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-143)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

98.211

99.466

0.977
  pilB Vibrio campbellii strain DS40M4

90.374

99.822

0.902
  pilB Vibrio cholerae strain A1552

73.843

100

0.738
  pilB Acinetobacter baumannii D1279779

51.407

94.84

0.488
  pilB Acinetobacter baylyi ADP1

48.108

98.754

0.475
  pilB Legionella pneumophila strain ERS1305867

48.98

95.907

0.47
  pilF Neisseria gonorrhoeae MS11

44.865

98.754

0.443
  pilB/pilB1 Synechocystis sp. PCC 6803

37.331

100

0.393
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.283

100

0.365
  pilF Thermus thermophilus HB27

36.738

99.288

0.365