Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   O2T11_RS13085 Genome accession   NZ_CP114186
Coordinates   2755768..2756232 (+) Length   154 a.a.
NCBI ID   WP_031857216.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain PH1273     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2750768..2761232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O2T11_RS13070 (O2T11_13070) pdhR 2752799..2753566 (-) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  O2T11_RS13075 (O2T11_13075) ampD 2753972..2754523 (-) 552 WP_005462574.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  O2T11_RS13080 (O2T11_13080) nadC 2754616..2755503 (+) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  O2T11_RS13085 (O2T11_13085) pilA 2755768..2756232 (+) 465 WP_031857216.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  O2T11_RS13090 (O2T11_13090) pilB 2756236..2757924 (+) 1689 WP_283658206.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O2T11_RS13095 (O2T11_13095) pilC 2757949..2759172 (+) 1224 WP_064490250.1 type II secretion system F family protein Machinery gene
  O2T11_RS13100 (O2T11_13100) pilD 2759237..2760106 (+) 870 WP_025622886.1 A24 family peptidase Machinery gene
  O2T11_RS13105 (O2T11_13105) coaE 2760107..2760721 (+) 615 WP_025633754.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16172.65 Da        Isoelectric Point: 8.1135

>NTDB_id=764921 O2T11_RS13085 WP_031857216.1 2755768..2756232(+) (pilA) [Vibrio parahaemolyticus strain PH1273]
MKHSKQKKQQGFTLIELMIVVAIIGILAAFAVPAYQNYTKKATLAEFPKVAAAMKLAVELCAHENASDATTFKSNCLSGS
NGVPVKITNLNDISIEAVAGSSGVNVIAQAVVVKGPIAAKEQYIMASNYTGSGIEWTSSCKDAGGKAQTDYCPE

Nucleotide


Download         Length: 465 bp        

>NTDB_id=764921 O2T11_RS13085 WP_031857216.1 2755768..2756232(+) (pilA) [Vibrio parahaemolyticus strain PH1273]
ATGAAACACAGTAAACAGAAAAAACAACAAGGTTTTACCTTGATTGAATTGATGATTGTAGTGGCTATTATCGGTATTTT
GGCTGCATTTGCCGTACCGGCATATCAAAACTATACAAAAAAAGCGACATTAGCGGAATTCCCCAAAGTAGCAGCAGCTA
TGAAATTAGCAGTTGAGTTGTGCGCACACGAAAATGCTTCGGATGCAACGACTTTTAAATCAAACTGTCTTTCCGGTAGT
AATGGGGTGCCTGTAAAGATTACTAACCTAAATGATATATCAATTGAAGCTGTCGCAGGGTCATCAGGTGTAAATGTTAT
TGCACAAGCAGTTGTAGTAAAAGGACCTATCGCAGCTAAAGAACAATACATAATGGCATCAAACTACACAGGAAGTGGCA
TTGAATGGACTAGCTCTTGTAAAGATGCAGGGGGTAAGGCTCAAACTGATTACTGCCCTGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

58.065

100

0.584
  pilA2 Legionella pneumophila strain ERS1305867

39.189

96.104

0.377
  pilA Haemophilus influenzae 86-028NP

40.714

90.909

0.37