Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H7R62_RS02115 Genome accession   NZ_AP022038
Coordinates   430361..431470 (+) Length   369 a.a.
NCBI ID   WP_111806244.1    Uniprot ID   A0A6S5CXX6
Organism   Aeromonas veronii strain WP3-W19-ESBL-03     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 425361..436470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R62_RS02085 (WP3W19E03_03880) - 426262..426681 (-) 420 WP_182938750.1 DUF4426 domain-containing protein -
  H7R62_RS02090 (WP3W19E03_03890) yggU 426725..427027 (-) 303 WP_005341417.1 DUF167 family protein YggU -
  H7R62_RS02095 (WP3W19E03_03900) - 427027..427578 (-) 552 WP_042011910.1 YggT family protein -
  H7R62_RS02100 (WP3W19E03_03910) proC 427590..428414 (-) 825 WP_182938751.1 pyrroline-5-carboxylate reductase -
  H7R62_RS02105 (WP3W19E03_03920) - 428557..429258 (-) 702 WP_182938752.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7R62_RS02110 (WP3W19E03_03930) pilT 429298..430332 (+) 1035 WP_042063177.1 type IVa pilus ATPase TapT Machinery gene
  H7R62_RS02115 (WP3W19E03_03940) pilU 430361..431470 (+) 1110 WP_111806244.1 type IVa pilus ATPase TapU Machinery gene
  H7R62_RS02120 (WP3W19E03_03950) yaaA 431518..432291 (+) 774 WP_182938753.1 peroxide stress protein YaaA -
  H7R62_RS02125 (WP3W19E03_03960) - 432486..432911 (+) 426 WP_042063174.1 hypothetical protein -
  H7R62_RS02130 (WP3W19E03_03970) - 433270..433761 (-) 492 WP_182938754.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  H7R62_RS02135 (WP3W19E03_03980) arfB 433790..434206 (-) 417 WP_075113904.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  H7R62_RS02140 (WP3W19E03_03990) glnK 434408..434746 (+) 339 WP_005341406.1 P-II family nitrogen regulator -
  H7R62_RS02145 (WP3W19E03_04000) - 435005..436012 (+) 1008 WP_197970805.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41051.94 Da        Isoelectric Point: 6.0891

>NTDB_id=76349 H7R62_RS02115 WP_111806244.1 430361..431470(+) (pilU) [Aeromonas veronii strain WP3-W19-ESBL-03]
MNLDELLTELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVIRRIETRIPTFEDLQLPNILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLQIKLSGQDTLGSGTLENVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=76349 H7R62_RS02115 WP_111806244.1 430361..431470(+) (pilU) [Aeromonas veronii strain WP3-W19-ESBL-03]
ATGAATCTGGATGAATTGCTGACCGAACTGGTCGAACGAAAGGGCTCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
TCTCAAGGTCAATGGTCATCTGGTGTCGCTGGGGGGCGAGGCGCTTGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTACATCCGTACCAAGGAGGCCAACTACGCCATCTATCGCGAGGCGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGATACGGCGCATTGAGACCCGTATCCCCACCTTTGA
AGATCTGCAGTTGCCCAACATCCTGCAGGAGGTCGCCATGGCCAAGCGGGGGTTGGTGCTGTTTGTCGGGGCCACCGGGG
CGGGTAAGTCCACCACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGCCATATCCTGACGGTGGAAGAC
CCGGTGGAGTTCGTCCATCAGCATGGCCGCAGTCTGGTGACCCAGCGTGAAGTGGGGATCGATACCGAGTCGTTCGATGT
GGCGCTGAAGAGCTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGTCACCTTTGCCTCGCCACTCTGCATGCCAACAACGCCAACCAGGCGCTGGATCGGATC
CTCCATCTGGTGCCGCAAGACAAACATCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGAAAGCCATCGTTGCCCAGCA
ACTGGTGCCAAGCATGGATGGCAAGCGGCGTTTTGCCGCGTTCGAGGTGCTGCTCAATACCCCGCTGATCACCGACATCA
TCCGCAAGGGTGAGATGCACCGCCTCAAAGAGGTGATGACCAAATCCACCGAACTGGGCATGCAGACCTTCGATCAGGCG
CTGTTCAGCCTGTTCTGTGCCGGCCAGATTGGCTACAGTGAGGCCCTCGCTCATGCCGACTCGGCTAATGATTTGCGTCT
GCAAATCAAGCTCTCTGGTCAGGATACGCTTGGCTCCGGCACGCTGGAGAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6S5CXX6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

61.413

99.729

0.612
  pilU Pseudomonas stutzeri DSM 10701

60.335

97.019

0.585
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.233

91.87

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36

Multiple sequence alignment