Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   H7R23_RS01690 Genome accession   NZ_AP021936
Coordinates   361921..362358 (+) Length   145 a.a.
NCBI ID   WP_004789794.1    Uniprot ID   A0A009QRP9
Organism   Acinetobacter pittii strain WP2-W18-ESBL-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 356921..367358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R23_RS01670 (WP2W18E11_03160) - 358303..359190 (+) 888 WP_057085853.1 metal-dependent hydrolase -
  H7R23_RS01675 (WP2W18E11_03170) - 359321..359911 (+) 591 WP_002117090.1 LemA family protein -
  H7R23_RS01680 (WP2W18E11_03180) - 359936..361018 (+) 1083 WP_017481724.1 TPM domain-containing protein -
  H7R23_RS01685 (WP2W18E11_03190) - 361012..361572 (+) 561 WP_057085854.1 TPM domain-containing protein -
  H7R23_RS01690 (WP2W18E11_03200) pilA 361921..362358 (+) 438 WP_004789794.1 pilin Machinery gene
  H7R23_RS01695 (WP2W18E11_03210) - 362416..363729 (+) 1314 WP_017481722.1 O-antigen ligase family protein -
  H7R23_RS01700 (WP2W18E11_03220) - 363835..365463 (+) 1629 WP_017481721.1 PglL family O-oligosaccharyltransferase -
  H7R23_RS01705 (WP2W18E11_03230) bfr 365506..365970 (-) 465 WP_005067113.1 heteropolymeric bacterioferritin subunit Bfr -
  H7R23_RS01710 (WP2W18E11_03240) bfd 366214..366408 (-) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  H7R23_RS01715 - 366511..366638 (+) 128 Protein_329 hypothetical protein -
  H7R23_RS01720 (WP2W18E11_03250) - 366628..367011 (-) 384 WP_002116996.1 RidA family protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14801.82 Da        Isoelectric Point: 8.1114

>NTDB_id=75962 H7R23_RS01690 WP_004789794.1 361921..362358(+) (pilA) [Acinetobacter pittii strain WP2-W18-ESBL-11]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQNYIAKSQVGTGLTDITAGKTNTETKLAEGLAANLSAASDVGLQSSSNAC
SSISVSVATTGLTTIECTLQGTSQINGKKISWERTADSATAGTTGTWRCKTDVATNLRPKSCGAS

Nucleotide


Download         Length: 438 bp        

>NTDB_id=75962 H7R23_RS01690 WP_004789794.1 361921..362358(+) (pilA) [Acinetobacter pittii strain WP2-W18-ESBL-11]
ATGAATGCACAAAAAGGTTTTACATTAATTGAGCTTATGATCGTGGTTGCCATTATTGGTATTTTGGCAGCAATTGCGAT
TCCTGCATATCAAAACTATATTGCTAAGTCTCAAGTAGGAACAGGTTTAACCGATATTACAGCTGGTAAAACTAATACAG
AAACTAAATTAGCAGAAGGTTTAGCAGCTAATTTATCTGCAGCATCTGACGTAGGACTACAAAGCTCGAGTAATGCTTGT
TCTTCTATTTCAGTATCTGTTGCTACTACTGGATTGACAACAATTGAATGTACATTGCAAGGTACTTCACAAATCAACGG
TAAGAAAATTTCTTGGGAACGAACAGCTGATAGTGCTACAGCAGGCACTACGGGTACATGGCGTTGTAAAACTGATGTTG
CAACAAATTTACGTCCTAAATCATGTGGTGCTTCGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009QRP9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

50.34

100

0.51
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.497

100

0.421
  pilA Vibrio cholerae strain A1552

41.497

100

0.421
  pilA Vibrio cholerae C6706

41.497

100

0.421
  comP Acinetobacter baylyi ADP1

40.397

100

0.421
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.958

98.621

0.414
  pilA Pseudomonas aeruginosa PAK

38.961

100

0.414
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.549

97.931

0.407
  pilA2 Legionella pneumophila strain ERS1305867

38.667

100

0.4

Multiple sequence alignment