Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ONZ66_RS19270 Genome accession   NZ_CP110647
Coordinates   4139896..4141005 (+) Length   369 a.a.
NCBI ID   WP_087757149.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain ZAS     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4134896..4146005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ONZ66_RS19235 (ONZ66_19240) - 4135485..4135784 (+) 300 WP_011899099.1 hypothetical protein -
  ONZ66_RS19240 (ONZ66_19245) - 4135853..4136272 (-) 420 WP_005320482.1 DUF4426 domain-containing protein -
  ONZ66_RS19245 (ONZ66_19250) yggU 4136291..4136590 (-) 300 WP_005320479.1 DUF167 family protein YggU -
  ONZ66_RS19250 (ONZ66_19255) - 4136590..4137141 (-) 552 WP_005320477.1 YggT family protein -
  ONZ66_RS19255 (ONZ66_19260) proC 4137170..4137994 (-) 825 WP_271773682.1 pyrroline-5-carboxylate reductase -
  ONZ66_RS19260 (ONZ66_19265) - 4138085..4138786 (-) 702 WP_271773683.1 YggS family pyridoxal phosphate-dependent enzyme -
  ONZ66_RS19265 (ONZ66_19270) pilT 4138826..4139860 (+) 1035 WP_005320466.1 type IVa pilus ATPase TapT Machinery gene
  ONZ66_RS19270 (ONZ66_19275) pilU 4139896..4141005 (+) 1110 WP_087757149.1 type IVa pilus ATPase TapU Machinery gene
  ONZ66_RS19275 (ONZ66_19280) yaaA 4141037..4141810 (+) 774 WP_271773684.1 peroxide stress protein YaaA -
  ONZ66_RS19280 (ONZ66_19285) srmB 4141999..4143222 (-) 1224 WP_005320462.1 ATP-dependent RNA helicase SrmB -
  ONZ66_RS19285 (ONZ66_19290) - 4143351..4144061 (+) 711 WP_270688178.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  ONZ66_RS19290 (ONZ66_19295) brnQ 4144247..4145560 (+) 1314 WP_257982297.1 branched-chain amino acid transport system II carrier protein -
  ONZ66_RS19295 (ONZ66_19300) - 4145639..4145869 (-) 231 WP_021140462.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41059.10 Da        Isoelectric Point: 6.3717

>NTDB_id=755660 ONZ66_RS19270 WP_087757149.1 4139896..4141005(+) (pilU) [Aeromonas salmonicida strain ZAS]
MNLDALLAELVARKGSDLFITVGTPPTLKINGHLFSLGSEALDKQSALTLVRETLSPEHFERYIRTKEGNYAIHREALGR
FRVSAFWQQELPGMVVRRIETRIPTFDELTLPPILQEVAMAKRGLVLFVGATGTGKSTTQAAMIGYRNQNADGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQLLFDLSFNLRAIVAQQLVPSLDGRRRFAAFEVLLNTPLITDIIRKGEVHRLKEVMTKSTELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQMGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=755660 ONZ66_RS19270 WP_087757149.1 4139896..4141005(+) (pilU) [Aeromonas salmonicida strain ZAS]
ATGAATCTGGATGCCCTGTTGGCCGAACTGGTCGCACGCAAAGGCTCGGATCTGTTCATCACGGTTGGGACGCCCCCCAC
CCTCAAGATCAACGGGCATCTGTTCTCCCTCGGCAGCGAAGCGCTCGACAAGCAGAGCGCGCTGACCCTGGTCAGGGAGA
CGCTCAGCCCTGAGCATTTCGAGCGCTACATCCGCACCAAGGAGGGCAACTACGCCATCCACCGCGAGGCACTGGGACGC
TTTCGGGTGAGTGCCTTCTGGCAGCAGGAGTTGCCGGGCATGGTGGTACGCCGTATCGAGACCCGCATTCCAACCTTCGA
CGAACTGACCCTGCCGCCCATCTTGCAAGAGGTGGCCATGGCCAAACGCGGCCTGGTGCTGTTCGTCGGTGCCACCGGAA
CGGGCAAGTCCACCACTCAGGCAGCCATGATCGGCTATCGAAACCAGAATGCGGATGGCCATATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGGGAAGTGGGTATAGATACGGAATCATTCGATGT
GGCGCTGAAGAGCTCCCTGCGCCAGGCACCTGACGTTATCCTCATCGGCGAGATCCGAAGCCAGGAAACCATGGAGTTCG
CACTGCAATTTGCCGAGACCGGCCACCTTTGCCTCGCGACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAGGAGAAACACCGTCAGTTACTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
ACTGGTGCCAAGCCTGGATGGTCGCCGCCGCTTCGCGGCCTTTGAAGTGCTGCTCAACACGCCGCTCATCACGGATATCA
TCCGCAAAGGCGAGGTTCATCGTCTCAAGGAGGTGATGACCAAGTCCACCGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTTACCCTGTTTTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCCGGCCGTGAGCAGATGGGTGCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.239

99.729

0.591
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

39.942

92.954

0.371
  pilT Legionella pneumophila strain ERS1305867

39.942

92.954

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36