Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ONZ54_RS19305 Genome accession   NZ_CP110645
Coordinates   4155955..4157064 (+) Length   369 a.a.
NCBI ID   WP_021140458.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain AS3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4150955..4162064
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ONZ54_RS19270 (ONZ54_19280) - 4151544..4151843 (+) 300 WP_011899099.1 hypothetical protein -
  ONZ54_RS19275 (ONZ54_19285) - 4151912..4152331 (-) 420 WP_005320482.1 DUF4426 domain-containing protein -
  ONZ54_RS19280 (ONZ54_19290) yggU 4152350..4152649 (-) 300 WP_005320479.1 DUF167 family protein YggU -
  ONZ54_RS19285 (ONZ54_19295) - 4152649..4153200 (-) 552 WP_005320477.1 YggT family protein -
  ONZ54_RS19290 (ONZ54_19300) proC 4153229..4154053 (-) 825 WP_271786935.1 pyrroline-5-carboxylate reductase -
  ONZ54_RS19295 (ONZ54_19305) - 4154144..4154845 (-) 702 WP_271786936.1 YggS family pyridoxal phosphate-dependent enzyme -
  ONZ54_RS19300 (ONZ54_19310) pilT 4154885..4155919 (+) 1035 WP_005320466.1 type IVa pilus ATPase TapT Machinery gene
  ONZ54_RS19305 (ONZ54_19315) pilU 4155955..4157064 (+) 1110 WP_021140458.1 type IVa pilus ATPase TapU Machinery gene
  ONZ54_RS19310 (ONZ54_19320) yaaA 4157096..4157869 (+) 774 WP_087757148.1 peroxide stress protein YaaA -
  ONZ54_RS19315 (ONZ54_19325) srmB 4158058..4159281 (-) 1224 WP_005320462.1 ATP-dependent RNA helicase SrmB -
  ONZ54_RS19320 (ONZ54_19330) - 4159410..4160120 (+) 711 WP_059112464.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  ONZ54_RS19325 (ONZ54_19335) brnQ 4160306..4161619 (+) 1314 WP_017412062.1 branched-chain amino acid transport system II carrier protein -
  ONZ54_RS19330 (ONZ54_19340) - 4161695..4161925 (-) 231 WP_005320453.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41087.11 Da        Isoelectric Point: 6.3717

>NTDB_id=755632 ONZ54_RS19305 WP_021140458.1 4155955..4157064(+) (pilU) [Aeromonas salmonicida strain AS3]
MNLDALLAELVARKGSDLFITVGTPPTLKINGHLFSLGSEALDKQSALTLVRETLSPEHFERYIRTREGNYAIHREALGR
FRVSAFWQQELPGMVVRRIETRIPTFDELTLPPILQEVAMAKRGLVLFVGATGTGKSTTQAAMIGYRNQNADGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQLLFDLSFNLRAIVAQQLVPSLDGRRRFAAFEVLLNTPLITDIIRKGEVHRLKEVMTKSTELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQMGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=755632 ONZ54_RS19305 WP_021140458.1 4155955..4157064(+) (pilU) [Aeromonas salmonicida strain AS3]
ATGAATCTGGATGCTCTGTTGGCCGAACTGGTCGCACGCAAAGGCTCGGATCTGTTCATCACGGTTGGGACGCCCCCCAC
CCTCAAGATCAACGGGCATCTGTTCTCCCTCGGCAGCGAAGCGCTCGACAAGCAGAGCGCGCTGACCCTGGTCAGGGAGA
CGCTCAGCCCTGAGCATTTCGAGCGCTACATCCGCACCAGGGAGGGCAACTACGCCATCCACCGCGAGGCACTGGGACGC
TTTCGGGTGAGTGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGGTGCGCCGTATCGAGACCCGCATTCCAACCTTTGA
CGAACTGACCCTGCCGCCCATCTTGCAGGAGGTGGCCATGGCCAAACGCGGCCTGGTGCTGTTCGTCGGTGCCACCGGAA
CGGGCAAATCCACCACTCAGGCAGCCATGATCGGCTATCGAAACCAGAATGCGGACGGCCATATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGGGAAGTGGGCATAGATACGGAATCATTCGATGT
GGCGCTGAAGAGCTCCCTGCGCCAGGCACCTGACGTTATCCTCATCGGCGAGATCCGAAGCCAGGAAACCATGGAGTTCG
CACTGCAATTTGCCGAGACCGGCCATCTTTGCCTCGCGACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTACCGCAGGAGAAACACCGCCAGTTACTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTCGCCCAGCA
ACTGGTGCCAAGCCTGGATGGTCGCCGCCGCTTCGCGGCGTTTGAAGTGCTGCTCAACACGCCGCTCATCACGGATATCA
TCCGCAAAGGCGAGGTTCATCGTCTCAAGGAGGTGATGACCAAATCCACCGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTTACCCTGTTTTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCCGGCCGTGAGCAGATGGGTGCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.511

99.729

0.593
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

39.942

92.954

0.371
  pilT Legionella pneumophila strain ERS1305867

39.942

92.954

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36