Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PALA35_RS25580 Genome accession   NZ_CP110346
Coordinates   5498997..5500214 (+) Length   405 a.a.
NCBI ID   WP_269974491.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5493997..5505214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA35_RS25555 (PALA35_05057) nadC 5494119..5494967 (+) 849 WP_269974489.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PALA35_RS25565 (PALA35_05059) - 5495147..5496532 (-) 1386 WP_020751089.1 O-antigen ligase family protein -
  PALA35_RS25570 (PALA35_05060) - 5496598..5497062 (-) 465 WP_124089607.1 pilin -
  PALA35_RS25575 (PALA35_05061) pilB 5497293..5498993 (+) 1701 WP_269974490.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA35_RS25580 (PALA35_05062) pilC 5498997..5500214 (+) 1218 WP_269974491.1 type II secretion system F family protein Machinery gene
  PALA35_RS25585 (PALA35_05063) pilD 5500215..5501087 (+) 873 WP_269974492.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PALA35_RS25590 (PALA35_05064) coaE 5501084..5501695 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PALA35_RS25595 (PALA35_05065) yacG 5501692..5501892 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PALA35_RS25600 (PALA35_05066) - 5501929..5502138 (-) 210 WP_003094660.1 hypothetical protein -
  PALA35_RS25605 (PALA35_05067) - 5502244..5502933 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  PALA35_RS25610 (PALA35_05068) - 5502930..5503400 (-) 471 WP_269974493.1 FAD/FMN-containing dehydrogenase -
  PALA35_RS25615 (PALA35_05069) - 5503397..5503822 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  PALA35_RS25620 (PALA35_05070) - 5503955..5504584 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  PALA35_RS25625 (PALA35_05071) - 5504581..5505030 (+) 450 WP_237504982.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44015.89 Da        Isoelectric Point: 9.8103

>NTDB_id=754239 PALA35_RS25580 WP_269974491.1 5498997..5500214(+) (pilC) [Pseudomonas aeruginosa strain PALA35]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPMDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVAGGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTMMVINISNVLQEWWLLVLLMMGGAGFLLN
HAYKRSEKFRDATDRTVLKLPIIGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVGKIKQDVSTGM
QLNFSMRTTNIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=754239 PALA35_RS25580 WP_269974491.1 5498997..5500214(+) (pilC) [Pseudomonas aeruginosa strain PALA35]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAAAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCA
TCTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCTATGGACATCGCCCTGTTCACCCGCCAGATGTCCACCATGATGGCC
GCCGGCGTGCCACTGCTGCAATCGTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTCGCCGGCGGCAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACT
GCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCGATCGCGGTGATCGTCGTCGCCATCATCGTTTCGGCGAT
CCTACTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTCGAGGGCTTCGGCGCCGAACTGCCAGCCTTCACCATGATGG
TCATCAACATTTCCAACGTACTCCAGGAATGGTGGCTCCTGGTACTGCTGATGATGGGCGGCGCCGGCTTCCTGCTGAAC
CACGCCTACAAGCGTTCGGAGAAGTTCCGCGACGCCACCGACCGCACGGTACTGAAGCTGCCGATCATCGGCGCCATCCT
CTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGTCGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGGGACGCGGTGGGCAAGATCAAGCAGGACGTGTCCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCAACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAATCGGG
CGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAG
CTGGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.519

100

0.785
  pilC Acinetobacter baylyi ADP1

60.784

100

0.612
  pilC Acinetobacter baumannii D1279779

60.396

99.753

0.602
  pilC Legionella pneumophila strain ERS1305867

54.545

97.778

0.533
  pilG Neisseria gonorrhoeae MS11

46.04

99.753

0.459
  pilG Neisseria meningitidis 44/76-A

45.522

99.259

0.452
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Vibrio campbellii strain DS40M4

40.648

99.012

0.402
  pilC Thermus thermophilus HB27

38.155

99.012

0.378