Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PALA37_RS16985 Genome accession   NZ_CP109850
Coordinates   3645652..3646869 (-) Length   405 a.a.
NCBI ID   WP_269974491.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA37     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3640652..3651869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA37_RS16940 (PALA37_03355) - 3640836..3641285 (-) 450 WP_003094670.1 MOSC domain-containing protein -
  PALA37_RS16945 (PALA37_03356) - 3641282..3641911 (-) 630 WP_003094668.1 DUF1780 domain-containing protein -
  PALA37_RS16950 (PALA37_03357) - 3642044..3642469 (+) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  PALA37_RS16955 (PALA37_03358) - 3642466..3642936 (+) 471 WP_003094664.1 hypothetical protein -
  PALA37_RS16960 (PALA37_03359) - 3642933..3643622 (+) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  PALA37_RS16965 (PALA37_03360) - 3643728..3643937 (+) 210 WP_003094660.1 hypothetical protein -
  PALA37_RS16970 (PALA37_03361) yacG 3643974..3644174 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PALA37_RS16975 (PALA37_03362) coaE 3644171..3644782 (-) 612 WP_127429831.1 dephospho-CoA kinase -
  PALA37_RS16980 (PALA37_03363) pilD 3644779..3645651 (-) 873 WP_270132262.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PALA37_RS16985 (PALA37_03364) pilC 3645652..3646869 (-) 1218 WP_269974491.1 type II secretion system F family protein Machinery gene
  PALA37_RS16990 (PALA37_03365) pilB 3646873..3648573 (-) 1701 WP_058355294.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA37_RS16995 (PALA37_03366) - 3648804..3649268 (+) 465 WP_128697780.1 pilin -
  PALA37_RS17000 (PALA37_03367) - 3649401..3650729 (+) 1329 WP_270132273.1 O-antigen ligase family protein -
  PALA37_RS17010 (PALA37_03369) nadC 3650910..3651758 (-) 849 WP_003125200.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44015.89 Da        Isoelectric Point: 9.8103

>NTDB_id=750123 PALA37_RS16985 WP_269974491.1 3645652..3646869(-) (pilC) [Pseudomonas aeruginosa strain PALA37]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPMDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVAGGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTMMVINISNVLQEWWLLVLLMMGGAGFLLN
HAYKRSEKFRDATDRTVLKLPIIGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVGKIKQDVSTGM
QLNFSMRTTNIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=750123 PALA37_RS16985 WP_269974491.1 3645652..3646869(-) (pilC) [Pseudomonas aeruginosa strain PALA37]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAAAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCA
TCTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCTATGGACATCGCCCTGTTCACCCGCCAGATGTCCACCATGATGGCC
GCCGGCGTGCCACTGCTGCAATCGTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTCGCCGGCGGCAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACT
GCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCGATCGCGGTGATCGTCGTCGCCATCATCGTTTCGGCGAT
CCTACTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTCGAGGGCTTCGGCGCCGAACTGCCAGCCTTCACCATGATGG
TCATCAACATTTCCAACGTACTCCAGGAATGGTGGCTCCTGGTACTGCTGATGATGGGCGGCGCCGGCTTCCTGCTGAAC
CACGCCTACAAGCGTTCGGAGAAGTTCCGCGACGCCACCGACCGCACGGTACTGAAGCTGCCGATCATCGGCGCCATCCT
CTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGTCGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGGGACGCGGTGGGCAAGATCAAGCAGGACGTGTCCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCAACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAATCGGG
CGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAG
CTGGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.519

100

0.785
  pilC Acinetobacter baylyi ADP1

60.784

100

0.612
  pilC Acinetobacter baumannii D1279779

60.396

99.753

0.602
  pilC Legionella pneumophila strain ERS1305867

54.545

97.778

0.533
  pilG Neisseria gonorrhoeae MS11

46.04

99.753

0.459
  pilG Neisseria meningitidis 44/76-A

45.522

99.259

0.452
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Vibrio campbellii strain DS40M4

40.648

99.012

0.402
  pilC Thermus thermophilus HB27

38.155

99.012

0.378