Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AZSP09_RS15140 Genome accession   NZ_AP021844
Coordinates   3221511..3222755 (-) Length   414 a.a.
NCBI ID   WP_014236420.1    Uniprot ID   A0A5H2YT80
Organism   Azospira sp. I09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3216511..3227755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AZSP09_RS15110 (AZSP09_29890) - 3216963..3217409 (-) 447 WP_152090505.1 barstar family protein -
  AZSP09_RS15115 (AZSP09_29900) - 3217413..3217805 (-) 393 WP_152090506.1 ribonuclease domain-containing protein -
  AZSP09_RS15120 (AZSP09_29910) aspS 3217821..3219632 (-) 1812 WP_152090507.1 aspartate--tRNA ligase -
  AZSP09_RS15125 (AZSP09_29920) - 3219663..3220283 (-) 621 WP_043798874.1 DUF502 domain-containing protein -
  AZSP09_RS15130 (AZSP09_29930) - 3220283..3220555 (-) 273 WP_130459327.1 zinc ribbon domain-containing protein -
  AZSP09_RS15135 (AZSP09_29940) pilD 3220654..3221508 (-) 855 WP_130459326.1 A24 family peptidase Machinery gene
  AZSP09_RS15140 (AZSP09_29950) pilC 3221511..3222755 (-) 1245 WP_014236420.1 type II secretion system F family protein Machinery gene
  AZSP09_RS15145 (AZSP09_29960) pilB 3222780..3224498 (-) 1719 WP_152090508.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AZSP09_RS15150 (AZSP09_29970) ccsA 3224774..3225613 (-) 840 WP_014236422.1 cytochrome c biogenesis protein CcsA -
  AZSP09_RS15155 (AZSP09_29980) ffh 3225659..3227014 (+) 1356 WP_152090509.1 signal recognition particle protein -

Sequence

Protein


Download         Length: 414 a.a.        Molecular weight: 45010.15 Da        Isoelectric Point: 9.8402

>NTDB_id=74943 AZSP09_RS15140 WP_014236420.1 3221511..3222755(-) (pilC) [Azospira sp. I09]
MATAGKNTQNTKRADPSIKEFVFVWEGKDKTGKIIRGELRATSETVVQATLRRQGVLVTKVSKQRFKSGGKITDKDITLF
TRQLATMMKSGVPLLQAFDIVGKGHSNPAVGKLLLDIKADVETGSALNQAFRKYPLYFDSLFCNLIGAGEQAGILDTLLD
RLAIYKEKILAVKSKIKSALFYPIAVIVVAFCITAVIMIFVIPAFKEVFKSFGADLPAPTLVVIAISDFFVAYWYLIFGI
LGGGIYSFLESWKRSEAVQIAMDKALLRMPIFGPLIRKSVIARWTRTLATMFAAGVPLVEALDSVGGASGNHVYKMATRQ
IQSEVSTGTSLTNSMQNVNLFPNMVIQMVAIGEESGALDSMLSKVADFFEAEVDDSVAALSSLMEPIIMVILGVLIGGMV
VAMYLPIFKMGSVV

Nucleotide


Download         Length: 1245 bp        

>NTDB_id=74943 AZSP09_RS15140 WP_014236420.1 3221511..3222755(-) (pilC) [Azospira sp. I09]
ATGGCCACTGCCGGCAAAAACACACAAAACACCAAGCGTGCCGACCCAAGCATCAAGGAGTTCGTCTTTGTTTGGGAAGG
CAAGGACAAGACCGGGAAAATCATCCGGGGGGAATTGCGCGCCACCAGCGAAACGGTGGTCCAGGCAACCCTGCGGCGCC
AAGGCGTGCTGGTTACCAAGGTATCCAAGCAGCGTTTCAAGAGTGGCGGCAAGATCACCGACAAGGACATCACCCTGTTC
ACCCGGCAGTTGGCCACCATGATGAAATCTGGGGTGCCGCTACTCCAGGCCTTCGACATCGTTGGCAAAGGCCACTCAAA
CCCCGCTGTCGGGAAACTGCTGCTGGATATCAAGGCTGATGTCGAAACGGGTAGCGCTCTGAACCAAGCTTTCCGCAAGT
ACCCACTTTACTTCGACAGCCTGTTCTGCAACTTGATCGGCGCCGGCGAGCAGGCCGGCATCCTCGATACACTCCTTGAT
CGGCTCGCCATCTACAAGGAAAAAATCCTAGCCGTCAAAAGCAAGATCAAGTCAGCCCTGTTCTATCCAATTGCCGTCAT
TGTCGTAGCTTTCTGCATTACGGCTGTGATCATGATTTTTGTAATTCCTGCGTTCAAAGAGGTTTTCAAATCGTTTGGTG
CGGACCTTCCTGCCCCGACACTGGTCGTAATAGCGATTTCTGACTTCTTCGTTGCCTATTGGTATTTGATCTTCGGCATA
TTGGGAGGAGGTATCTATTCCTTCCTGGAGTCGTGGAAGCGCTCCGAGGCCGTTCAGATTGCCATGGACAAGGCATTGCT
GAGGATGCCGATTTTCGGCCCCCTCATACGGAAATCGGTTATCGCCAGATGGACGAGAACCCTGGCAACCATGTTTGCCG
CCGGCGTTCCTCTGGTTGAGGCATTGGACTCAGTGGGCGGAGCCTCAGGCAACCACGTTTACAAAATGGCAACGCGTCAG
ATCCAAAGCGAAGTCAGTACCGGCACCAGCCTGACCAACTCAATGCAGAATGTGAACCTGTTCCCCAATATGGTGATCCA
GATGGTTGCCATCGGTGAGGAATCTGGAGCCCTCGACTCAATGCTGTCCAAGGTTGCGGACTTCTTTGAAGCCGAAGTAG
ACGATTCTGTTGCGGCCCTCTCCAGCCTGATGGAGCCAATCATCATGGTCATATTGGGGGTTCTCATCGGCGGCATGGTG
GTGGCGATGTACCTGCCGATCTTCAAAATGGGCTCCGTGGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(80-203)

Type II secretion system (T2SS), protein F (T2SSF)

(284-406)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5H2YT80

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

55.808

95.652

0.534
  pilC Pseudomonas stutzeri DSM 10701

53.713

97.585

0.524
  pilC Acinetobacter baumannii D1279779

53.383

96.377

0.514
  pilC Acinetobacter baylyi ADP1

52.01

96.135

0.5
  pilG Neisseria gonorrhoeae MS11

51.889

95.894

0.498
  pilG Neisseria meningitidis 44/76-A

51.637

95.894

0.495
  pilC Vibrio campbellii strain DS40M4

38.725

98.551

0.382
  pilC Vibrio cholerae strain A1552

38.636

95.652

0.37

Multiple sequence alignment