Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AZSP09_RS15145 Genome accession   NZ_AP021844
Coordinates   3222780..3224498 (-) Length   572 a.a.
NCBI ID   WP_152090508.1    Uniprot ID   A0A5H2Y9L3
Organism   Azospira sp. I09     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3217780..3229498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AZSP09_RS15120 (AZSP09_29910) aspS 3217821..3219632 (-) 1812 WP_152090507.1 aspartate--tRNA ligase -
  AZSP09_RS15125 (AZSP09_29920) - 3219663..3220283 (-) 621 WP_043798874.1 DUF502 domain-containing protein -
  AZSP09_RS15130 (AZSP09_29930) - 3220283..3220555 (-) 273 WP_130459327.1 zinc ribbon domain-containing protein -
  AZSP09_RS15135 (AZSP09_29940) pilD 3220654..3221508 (-) 855 WP_130459326.1 A24 family peptidase Machinery gene
  AZSP09_RS15140 (AZSP09_29950) pilC 3221511..3222755 (-) 1245 WP_014236420.1 type II secretion system F family protein Machinery gene
  AZSP09_RS15145 (AZSP09_29960) pilB 3222780..3224498 (-) 1719 WP_152090508.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AZSP09_RS15150 (AZSP09_29970) ccsA 3224774..3225613 (-) 840 WP_014236422.1 cytochrome c biogenesis protein CcsA -
  AZSP09_RS15155 (AZSP09_29980) ffh 3225659..3227014 (+) 1356 WP_152090509.1 signal recognition particle protein -
  AZSP09_RS15160 (AZSP09_29990) - 3227334..3227942 (-) 609 WP_130459324.1 lytic transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62269.47 Da        Isoelectric Point: 5.3501

>NTDB_id=74944 AZSP09_RS15145 WP_152090508.1 3222780..3224498(-) (pilB) [Azospira sp. I09]
MAATFQVPLSGLARAMVQAGRLKEAEAEAFMAQASEKGTTFIEQVIAGKRASAREIAVFAGETFGYPVLDLSAFDEANIP
ASAIDRKLIATHRVIPLNKRGNRLSVAIADPTNLRALDEIRFQTGLAVDSVVVEEPKLAPLVVKYSESAEENLKNLTNED
LNLEFAEEEDQAKADEAVSLDVDDAPVVKFIQKILLDAINDGASDIHFEPYEKFYRIRYRIDGELREVANPPLAIKEKVA
SRIKVISRMDISEKRVPQDGRMKLVLSKTRAIDFRVSTLPTLHGEKIVMRILDPSSATLGIEALGYEPEQKEALLNAIHR
PYGMVLVTGPTGSGKTVSLYTCLNILNKPGINISTAEDPAEINLAGINQVNVNDKAGLTFSAALKSFLRQDPDIIMVGEI
RDLDTAEIAIKAAQTGHMVLSTLHTNDAPTTLERLKNMGVPTFNIASSVILITAQRLARRLCNCKVPLHVPTETLLNAGF
TEAELDGSWTLYGPNPEGCERCKGGGFKGRVGIYQVMPVSEEIQRIIMANGNSMDIAAQAKKEGVKDLRQSGLLKVKQGV
TSLDEVLSTTNA

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=74944 AZSP09_RS15145 WP_152090508.1 3222780..3224498(-) (pilB) [Azospira sp. I09]
ATGGCAGCTACTTTTCAGGTTCCCCTAAGTGGTCTTGCGCGCGCCATGGTCCAGGCTGGACGCCTCAAAGAGGCCGAAGC
CGAAGCCTTCATGGCCCAAGCCAGCGAAAAGGGCACGACATTCATTGAGCAGGTGATCGCCGGCAAGCGGGCCAGCGCCA
GGGAAATAGCCGTATTCGCAGGAGAAACATTCGGCTACCCGGTCCTTGATCTGAGCGCTTTCGATGAAGCCAACATTCCG
GCCAGCGCCATTGACCGGAAGCTCATCGCCACCCACCGCGTCATACCGCTGAACAAGCGCGGCAACCGGCTCTCGGTAGC
GATAGCTGACCCAACAAATTTGCGGGCCTTGGACGAAATTCGCTTCCAGACAGGCTTGGCAGTGGACTCCGTCGTTGTCG
AAGAGCCCAAACTGGCACCGCTGGTCGTCAAATATTCGGAATCGGCAGAAGAAAACCTCAAGAATCTCACCAATGAGGAC
TTGAACCTCGAATTTGCGGAAGAAGAAGATCAGGCCAAGGCCGATGAAGCCGTTTCCCTGGATGTGGATGACGCACCGGT
CGTCAAGTTCATTCAGAAAATACTGCTGGATGCGATCAACGATGGCGCTTCCGACATCCACTTCGAGCCGTATGAAAAGT
TTTACCGCATCCGCTATCGCATCGACGGCGAGTTGCGAGAGGTTGCCAATCCGCCCCTGGCAATCAAGGAAAAGGTGGCT
TCCCGCATCAAGGTCATTTCCCGGATGGACATTTCCGAGAAGCGGGTCCCGCAAGACGGCCGGATGAAGCTGGTCCTTTC
CAAAACCAGAGCCATCGACTTCCGGGTCAGCACGCTACCAACGCTGCATGGCGAAAAGATCGTCATGCGTATTCTGGATC
CGTCCTCCGCCACGCTGGGCATCGAAGCGCTGGGCTACGAGCCGGAACAGAAGGAAGCCCTGCTCAACGCCATCCACCGT
CCTTATGGCATGGTTCTGGTAACCGGCCCGACCGGCTCCGGTAAAACCGTATCCTTGTACACCTGCCTCAACATCCTGAA
CAAGCCTGGCATCAACATCTCCACCGCGGAAGACCCGGCAGAAATCAACCTGGCCGGCATCAACCAGGTAAACGTCAACG
ACAAGGCAGGTCTGACCTTTTCCGCCGCCCTCAAGTCCTTCCTGCGGCAGGATCCGGACATCATCATGGTGGGTGAAATC
CGCGACCTGGACACTGCCGAAATCGCAATCAAGGCAGCCCAGACCGGCCACATGGTGCTATCCACCTTGCACACCAACGA
TGCCCCGACGACACTGGAGCGCCTGAAGAACATGGGCGTCCCCACCTTCAACATTGCCTCGTCCGTCATCCTGATCACCG
CCCAGCGGCTGGCCCGCCGTCTGTGCAACTGCAAGGTCCCGCTCCACGTCCCCACCGAAACCCTTCTGAATGCAGGCTTT
ACCGAAGCAGAACTGGATGGCAGTTGGACACTCTATGGCCCCAACCCGGAAGGATGCGAGCGCTGCAAGGGAGGTGGTTT
CAAGGGCCGGGTAGGCATTTACCAGGTGATGCCGGTTTCCGAGGAAATTCAGCGCATCATCATGGCCAATGGCAACTCCA
TGGATATTGCTGCCCAGGCCAAAAAGGAAGGGGTCAAGGACTTGCGTCAGTCCGGCCTGTTGAAAGTAAAGCAGGGAGTC
ACCTCCCTGGACGAAGTGCTGTCTACGACAAACGCCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-151)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5H2Y9L3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.401

100

0.556
  pilB Acinetobacter baylyi ADP1

54.939

100

0.554
  pilB Legionella pneumophila strain ERS1305867

53.322

100

0.533
  pilF Neisseria gonorrhoeae MS11

52.91

99.126

0.524
  pilB Vibrio cholerae strain A1552

49.558

98.776

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

47.687

98.252

0.469
  pilB Vibrio campbellii strain DS40M4

47.08

98.776

0.465
  pilF Thermus thermophilus HB27

38.489

99.476

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.769

90.909

0.371

Multiple sequence alignment