Detailed information NTG0737959

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: OD347_RS12570
• Genome accession: NZ_CP107079
• Coordinates: 2501042..2501656 (-)
• Length: 204 a.a.
• NCBI ID: WP_088613149.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain LT-2
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2496042..2506656

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OD347_RS12540	rpsT	2496342..2496608 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
OD347_RS12545	holA	2496626..2497667 (-)	1042	Protein_2429	DNA polymerase III subunit delta	-
OD347_RS12550	-	2497707..2497861 (-)	155	Protein_2430	hypothetical protein	-
OD347_RS12555	-	2497902..2498036 (+)	135	WP_003152870.1	YqzM family protein	-
OD347_RS12560	comEC	2498055..2500406 (-)	2352	WP_088613147.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
OD347_RS12565	-	2500407..2500976 (-)	570	WP_088613148.1	ComE operon protein 2	-
OD347_RS12570	comEA	2501042..2501656 (-)	615	WP_088613149.1	helix-hairpin-helix domain-containing protein	Machinery gene
OD347_RS12575	comER	2501716..2502537 (+)	822	WP_088613150.1	late competence protein ComER	-
OD347_RS12580	-	2502606..2503343 (-)	738	WP_088613151.1	class I SAM-dependent methyltransferase	-
OD347_RS12585	rsfS	2503340..2503696 (-)	357	WP_045510454.1	ribosome silencing factor	-
OD347_RS12590	yqeK	2503713..2504273 (-)	561	WP_088613152.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
OD347_RS12595	-	2504263..2504832 (-)	570	WP_045510448.1	nicotinate-nucleotide adenylyltransferase	-
OD347_RS12600	yhbY	2504843..2505133 (-)	291	WP_014470707.1	ribosome assembly RNA-binding protein YhbY	-
OD347_RS12605	aroE	2505127..2505969 (-)	843	WP_014470708.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22271.52 Da
• Isoelectric Point: 6.0432

>NTDB_id=737959 OD347_RS12570 WP_088613149.1 2501042..2501656(-) (comEA) [Bacillus velezensis strain LT-2]
MTKVMMTERQRQYLKILGVCLAAGAVFICILLFRPEKEEPVKQQSVLGQTASAPVTDKKDTADDKIMIDIKGAVRHPGVY
EMKAGDRVTQAIKKAGGLKEKADELNVNLAEQLQDGTVVYIPSEGEKENPTKTAAGEKENASVNINTASLDELQAIPGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=737959 OD347_RS12570 WP_088613149.1 2501042..2501656(-) (comEA) [Bacillus velezensis strain LT-2]
ATGACGAAAGTGATGATGACGGAGCGGCAAAGGCAGTATCTGAAAATTCTAGGCGTTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTTGCTGTTTCGTCCCGAAAAAGAAGAGCCTGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCTTCAGCAC
CCGTCACGGACAAAAAAGACACCGCAGACGATAAAATTATGATTGATATTAAAGGAGCGGTCCGACATCCCGGCGTGTAT
GAAATGAAGGCGGGGGACAGAGTGACGCAAGCCATTAAGAAAGCCGGCGGTCTGAAGGAAAAGGCGGATGAACTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGAACGGTCGTTTATATCCCGAGTGAAGGCGAAAAGGAGAATCCCACGAAAACCG
CAGCAGGTGAAAAGGAGAATGCTTCTGTCAATATCAATACGGCATCTCTTGACGAACTTCAGGCGATTCCCGGTGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGGCGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGTTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	57.949	95.588	0.554
comEA	Lactococcus lactis subsp. cremoris KW2	35.455	100	0.382
comEA	Staphylococcus aureus MW2	35.294	100	0.382
comEA/celA/cilE	Streptococcus pneumoniae D39	35.484	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae R6	35.484	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.484	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.484	100	0.377
comEA/celA/cilE	Streptococcus mitis SK321	35.023	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	34.562	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	32.051	100	0.368