Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   P4B24_RS26940 Genome accession   NZ_CP120708
Coordinates   5677516..5677980 (-) Length   154 a.a.
NCBI ID   WP_003122079.1    Uniprot ID   P17836
Organism   Pseudomonas aeruginosa strain NY7723     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5672516..5682980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4B24_RS26920 (P4B24_26925) - 5672532..5674373 (+) 1842 WP_023109352.1 ClpP-like prohead protease/major capsid protein fusion protein -
  P4B24_RS26925 (P4B24_26930) - 5675554..5675796 (+) 243 WP_023109353.1 hypothetical protein -
  P4B24_RS26935 (P4B24_26940) - 5676055..5677440 (-) 1386 WP_003122080.1 O-antigen ligase -
  P4B24_RS26940 (P4B24_26945) pilA 5677516..5677980 (-) 465 WP_003122079.1 pilin Machinery gene
  P4B24_RS26945 (P4B24_26950) pilB 5678211..5679911 (+) 1701 WP_003122078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P4B24_RS26950 (P4B24_26955) pilC 5679915..5681132 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  P4B24_RS26955 (P4B24_26960) pilD 5681133..5682005 (+) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  P4B24_RS26960 (P4B24_26965) coaE 5682002..5682613 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  P4B24_RS26965 (P4B24_26970) yacG 5682610..5682810 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16277.67 Da        Isoelectric Point: 8.9987

>NTDB_id=734417 P4B24_RS26940 WP_003122079.1 5677516..5677980(-) (pilA) [Pseudomonas aeruginosa strain NY7723]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSATPKDTQYDIGFTE
STLLDGSGKSQIQVTDNKDGTVELVATLGKSSGSAIKGAVITVSRKNDGVWNCKITKTPTAWKPNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=734417 P4B24_RS26940 WP_003122079.1 5677516..5677980(-) (pilA) [Pseudomonas aeruginosa strain NY7723]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTTGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAGGAGATTGTTTCCAGCGCGACTCCTAAAGATACCCAGTATGACATTGGCTTCACCGAG
TCTACTTTGCTAGATGGTTCTGGTAAGAGTCAGATCCAGGTAACGGACAATAAAGATGGCACCGTTGAGTTGGTCGCTAC
CTTGGGTAAATCTTCTGGTTCCGCTATCAAAGGGGCTGTAATCACTGTTTCGCGTAAAAATGACGGAGTCTGGAACTGCA
AAATCACCAAAACTCCTACAGCTTGGAAGCCCAACTACGCTCCGGCTAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 8V7P

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

44.737

98.701

0.442
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.444

99.351

0.442
  pilA Pseudomonas aeruginosa PAK

45.985

88.961

0.409
  pilA2 Legionella pneumophila strain ERS1305867

39.073

98.052

0.383
  pilA Vibrio parahaemolyticus RIMD 2210633

37.255

99.351

0.37
  pilA2 Legionella pneumophila str. Paris

38

97.403

0.37
  pilA Vibrio cholerae strain A1552

36.774

100

0.37
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.774

100

0.37
  pilA Vibrio cholerae C6706

36.774

100

0.37