Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   N7D90_RS03700 Genome accession   NZ_CP104861
Coordinates   766455..767672 (-) Length   405 a.a.
NCBI ID   WP_261740928.1    Uniprot ID   -
Organism   Pseudomonas fragi strain D12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 761455..772672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7D90_RS03665 (N7D90_03665) - 761609..762910 (-) 1302 WP_261740922.1 NAD(P)/FAD-dependent oxidoreductase -
  N7D90_RS03670 (N7D90_03670) - 762976..763149 (-) 174 WP_261740923.1 DUF3094 domain-containing protein -
  N7D90_RS03675 (N7D90_03675) - 763240..763869 (-) 630 WP_261740924.1 DUF1780 domain-containing protein -
  N7D90_RS03680 (N7D90_03680) - 763979..764668 (+) 690 WP_261740925.1 energy-coupling factor ABC transporter permease -
  N7D90_RS03685 (N7D90_03685) yacG 764767..764970 (-) 204 WP_261740926.1 DNA gyrase inhibitor YacG -
  N7D90_RS03690 (N7D90_03690) coaE 764967..765590 (-) 624 WP_261740927.1 dephospho-CoA kinase -
  N7D90_RS03695 (N7D90_03695) pilD 765592..766452 (-) 861 WP_315968150.1 prepilin peptidase Machinery gene
  N7D90_RS03700 (N7D90_03700) pilC 766455..767672 (-) 1218 WP_261740928.1 type II secretion system F family protein Machinery gene
  N7D90_RS03705 (N7D90_03705) - 767886..768293 (+) 408 WP_261740929.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  N7D90_RS03715 (N7D90_03715) - 768531..770021 (+) 1491 WP_261740930.1 DNA-3-methyladenine glycosylase 2 -
  N7D90_RS03720 (N7D90_03720) - 770024..770521 (+) 498 WP_261740931.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  N7D90_RS03725 (N7D90_03725) nadC 770639..771487 (-) 849 WP_261740932.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44123.17 Da        Isoelectric Point: 9.7985

>NTDB_id=732810 N7D90_RS03700 WP_261740928.1 766455..767672(-) (pilC) [Pseudomonas fragi strain D12]
MATQAVKTRLYRWQGTDLKGARISGQITADSHVMIRVQLRRQGITVSKIRCHANHQCPGAKSISAVDITLFTRQLATLIN
AGIPLLQALEVIANGFEQRAMRQLVTELKQDIGAGTSLAAALRKKPAHFDLLFCSLVEAGELAGALDVLLLRVATYKEKT
EALKKKIRKAMTYPIAVLLVALAVSSILLIEVVPQFQGMFSSFDAQLPVFTLWVIGLSEVLQTHGLWLLGFFCVTLACLR
HRYKHSVLLRQRADRWLLRLPVIGNLLHLSAIARFARTLSTTIAAGVPLLEALNSVASATGNSLYKEAVLRLKQQVATGS
QIHVAMGATGVFPGMAIQMTAIGEESGTLDDMLDHVATYYESEVDTLVDSLTTLMEPAIMVVLGIMVGGLVIAMYLPIFK
LGQVI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=732810 N7D90_RS03700 WP_261740928.1 766455..767672(-) (pilC) [Pseudomonas fragi strain D12]
ATGGCAACACAGGCCGTTAAAACCAGGCTTTATCGATGGCAGGGCACTGATCTCAAGGGGGCTCGAATTAGTGGTCAAAT
CACCGCAGACAGTCACGTAATGATCCGGGTCCAACTGCGCCGCCAAGGTATTACCGTTAGTAAAATCCGCTGCCATGCAA
ACCATCAGTGTCCAGGCGCGAAATCCATCAGTGCTGTGGATATCACCCTGTTCACCCGTCAGTTGGCCACCCTGATCAAC
GCCGGTATCCCGCTACTGCAAGCCCTGGAGGTTATCGCCAACGGTTTCGAGCAGCGCGCAATGCGCCAACTGGTGACCGA
ACTCAAACAGGACATCGGTGCAGGCACCAGCCTGGCGGCTGCGCTGCGCAAAAAACCGGCACATTTTGACCTGCTGTTTT
GCAGTCTCGTTGAAGCAGGGGAGCTGGCTGGGGCTCTGGATGTGTTGCTGCTGCGGGTGGCCACCTACAAGGAAAAAACC
GAAGCCCTGAAAAAGAAAATCAGAAAGGCCATGACCTACCCCATCGCCGTATTGCTGGTGGCATTAGCGGTGAGCAGCAT
TCTGTTGATCGAAGTGGTGCCGCAATTTCAGGGCATGTTTTCCTCATTCGACGCACAACTCCCCGTGTTCACGTTGTGGG
TAATCGGCCTGTCAGAGGTACTGCAAACACACGGCCTGTGGCTGCTGGGATTTTTTTGCGTCACGCTTGCCTGCCTGCGC
CATCGCTATAAACACTCCGTCCTTCTGCGCCAGCGCGCTGATCGATGGCTTCTGAGGTTGCCCGTCATCGGCAACCTGTT
GCACTTGTCTGCAATAGCCCGCTTCGCCCGCACGCTGTCGACCACCATTGCGGCTGGAGTGCCGCTGCTTGAGGCGTTGA
ACTCTGTGGCCAGTGCTACTGGCAATAGCCTGTACAAAGAAGCGGTGCTACGGCTCAAACAGCAGGTGGCGACGGGCAGC
CAGATCCATGTGGCCATGGGTGCTACCGGGGTGTTCCCTGGCATGGCGATTCAAATGACAGCAATTGGCGAAGAATCCGG
CACACTGGACGATATGCTTGACCACGTAGCCACCTATTATGAAAGCGAGGTCGACACTCTGGTGGATAGCCTCACAACCT
TGATGGAGCCGGCAATCATGGTGGTACTTGGGATCATGGTGGGCGGACTGGTAATCGCCATGTATTTACCGATTTTCAAA
CTGGGCCAAGTGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.074

100

0.541
  pilC Acinetobacter baumannii D1279779

50.126

98.025

0.491
  pilC Acinetobacter baylyi ADP1

50

98.272

0.491
  pilC Legionella pneumophila strain ERS1305867

49.373

98.519

0.486
  pilC Vibrio cholerae strain A1552

41.363

100

0.42
  pilC Vibrio campbellii strain DS40M4

39.32

100

0.4
  pilG Neisseria meningitidis 44/76-A

40.052

95.556

0.383
  pilG Neisseria gonorrhoeae MS11

39.487

96.296

0.38
  pilC Thermus thermophilus HB27

36.881

99.753

0.368