Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssb   Type   Machinery gene
Locus tag   N7M47_RS01935 Genome accession   NZ_CP104856
Coordinates   378201..378659 (+) Length   152 a.a.
NCBI ID   WP_002365911.1    Uniprot ID   Q8VT46
Organism   Enterococcus faecalis strain NY13412     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 369789..428683 378201..378659 within 0


Gene organization within MGE regions


Location: 369789..428683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7M47_RS01870 (N7M47_01870) prgU 370342..370698 (+) 357 WP_010714280.1 pheromone response system RNA-binding regulator PrgU -
  N7M47_RS01875 (N7M47_01875) - 370801..371106 (+) 306 WP_224567327.1 hypothetical protein -
  N7M47_RS01880 (N7M47_01880) - 371252..371512 (+) 261 WP_002370667.1 hypothetical protein -
  N7M47_RS01885 (N7M47_01885) - 371523..372401 (+) 879 WP_002377931.1 hypothetical protein -
  N7M47_RS01890 (N7M47_01890) - 372421..373281 (+) 861 WP_010710128.1 LPXTG cell wall anchor domain-containing protein -
  N7M47_RS01895 (N7M47_01895) - 373307..374577 (+) 1271 Protein_351 CHAP domain-containing protein -
  N7M47_RS01900 (N7M47_01900) - 374580..375197 (+) 618 WP_002377936.1 hypothetical protein -
  N7M47_RS01905 (N7M47_01905) - 375181..375597 (+) 417 WP_002401419.1 thioredoxin domain-containing protein -
  N7M47_RS01910 (N7M47_01910) - 375597..375911 (+) 315 WP_002360796.1 hypothetical protein -
  N7M47_RS01915 (N7M47_01915) - 375904..376938 (+) 1035 WP_002403100.1 conjugal transfer protein -
  N7M47_RS01920 (N7M47_01920) - 376943..377203 (+) 261 WP_010706916.1 hypothetical protein -
  N7M47_RS01925 (N7M47_01925) - 377203..377592 (+) 390 WP_002360791.1 TcpE family conjugal transfer membrane protein -
  N7M47_RS01930 (N7M47_01930) - 377624..378106 (+) 483 WP_002377938.1 hypothetical protein -
  N7M47_RS01935 (N7M47_01935) ssb 378201..378659 (+) 459 WP_002365911.1 single-stranded DNA-binding protein Machinery gene
  N7M47_RS01940 (N7M47_01940) - 378764..381256 (+) 2493 WP_002377939.1 ATP-binding protein -
  N7M47_RS01945 (N7M47_01945) - 381213..381611 (+) 399 WP_002370287.1 DUF4923 family protein -
  N7M47_RS01950 (N7M47_01950) - 381624..383969 (+) 2346 WP_002377940.1 MAEBL family membrane protein -
  N7M47_RS01955 (N7M47_01955) - 383956..386214 (+) 2259 WP_010710132.1 TraM recognition domain-containing protein -
  N7M47_RS01960 (N7M47_01960) - 386737..387522 (+) 786 WP_002360778.1 replication-relaxation family protein -
  N7M47_RS01965 (N7M47_01965) - 387528..388016 (+) 489 WP_010710133.1 hypothetical protein -
  N7M47_RS01970 (N7M47_01970) - 388608..388874 (+) 267 WP_002377947.1 hypothetical protein -
  N7M47_RS01975 (N7M47_01975) - 388907..389407 (+) 501 WP_002360775.1 DnaJ domain-containing protein -
  N7M47_RS01980 (N7M47_01980) - 389420..389668 (+) 249 WP_002360773.1 DUF3850 domain-containing protein -
  N7M47_RS01985 (N7M47_01985) - 389744..390160 (+) 417 WP_010710134.1 single-stranded DNA-binding protein -
  N7M47_RS01990 (N7M47_01990) - 390184..390768 (+) 585 WP_002377949.1 thermonuclease family protein -
  N7M47_RS01995 (N7M47_01995) - 390879..391145 (+) 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
  N7M47_RS02000 (N7M47_02000) - 391138..391413 (+) 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin -
  N7M47_RS02005 (N7M47_02005) - 391604..392248 (-) 645 WP_002377952.1 IS6 family transposase -
  N7M47_RS02010 (N7M47_02010) - 392381..393263 (+) 883 Protein_374 IS256 family transposase -
  N7M47_RS02015 (N7M47_02015) - 393568..394605 (-) 1038 WP_002355369.1 PTS sugar transporter subunit IIC -
  N7M47_RS02020 (N7M47_02020) - 394631..395569 (-) 939 WP_002370935.1 2-dehydropantoate 2-reductase -
  N7M47_RS02025 (N7M47_02025) - 395697..395966 (-) 270 Protein_377 LPXTG cell wall anchor domain-containing protein -
  N7M47_RS02030 (N7M47_02030) - 396056..396241 (+) 186 WP_002358660.1 hypothetical protein -
  N7M47_RS02035 (N7M47_02035) - 396273..396881 (+) 609 Protein_379 IS6 family transposase -
  N7M47_RS02040 (N7M47_02040) bsh 397550..398524 (-) 975 WP_002355428.1 choloylglycine hydrolase -
  N7M47_RS02045 (N7M47_02045) - 398748..399392 (+) 645 WP_002377952.1 IS6 family transposase -
  N7M47_RS02050 (N7M47_02050) - 399565..399879 (+) 315 WP_002403043.1 hypothetical protein -
  N7M47_RS02055 (N7M47_02055) cylR2 400217..400417 (-) 201 WP_002370931.1 cytolysin regulator CylR2 -
  N7M47_RS02060 (N7M47_02060) cylL-L 400821..401027 (+) 207 WP_002358485.1 lanthipeptide cytolysin subunit CylL-L -
  N7M47_RS02065 (N7M47_02065) cylL-S 401061..401252 (+) 192 WP_002358181.1 lanthipeptide cytolysin subunit CylL-S -
  N7M47_RS02070 (N7M47_02070) - 401313..404294 (+) 2982 WP_002370929.1 type 2 lanthipeptide synthetase LanM family protein -
  N7M47_RS02075 (N7M47_02075) - 404306..406450 (+) 2145 WP_002370927.1 peptidase domain-containing ABC transporter -
  N7M47_RS02080 (N7M47_02080) - 406447..407685 (+) 1239 WP_002368283.1 S8 family serine peptidase -
  N7M47_RS02085 (N7M47_02085) - 407686..408669 (+) 984 WP_128701265.1 site-2 protease family protein -
  N7M47_RS02090 (N7M47_02090) - 409022..409585 (+) 564 WP_002370925.1 hypothetical protein -
  N7M47_RS02095 (N7M47_02095) - 410022..410489 (+) 468 WP_002370921.1 hypothetical protein -
  N7M47_RS02100 (N7M47_02100) - 410778..411149 (+) 372 WP_242881059.1 hypothetical protein -
  N7M47_RS02105 (N7M47_02105) - 411244..411450 (+) 207 WP_229017897.1 S41 family peptidase -
  N7M47_RS02110 (N7M47_02110) amaP 411720..412286 (+) 567 WP_010706722.1 alkaline shock response membrane anchor protein AmaP -
  N7M47_RS02115 (N7M47_02115) - 412303..412494 (+) 192 WP_002358517.1 DUF2273 domain-containing protein -
  N7M47_RS02120 (N7M47_02120) - 412523..413059 (+) 537 WP_002358518.1 Asp23/Gls24 family envelope stress response protein -
  N7M47_RS02125 (N7M47_02125) - 413334..418703 (+) 5370 WP_102970081.1 Rib/alpha-like domain-containing protein -
  N7M47_RS02130 (N7M47_02130) - 418903..420111 (+) 1209 WP_002358521.1 YSIRK-targeted surface antigen transcriptional regulator -
  N7M47_RS02135 (N7M47_02135) - 420241..420657 (+) 417 WP_002358522.1 hypothetical protein -
  N7M47_RS02140 (N7M47_02140) - 420793..420894 (-) 102 Protein_400 IS30 family transposase -
  N7M47_RS02145 (N7M47_02145) - 420882..421619 (+) 738 WP_002401428.1 hypothetical protein -
  N7M47_RS02150 (N7M47_02150) - 421665..422285 (-) 621 WP_033591050.1 recombinase family protein -
  N7M47_RS02155 (N7M47_02155) - 422469..423088 (+) 620 Protein_403 recombinase family protein -
  N7M47_RS02160 (N7M47_02160) - 423197..423379 (+) 183 WP_002358529.1 hypothetical protein -
  N7M47_RS02165 (N7M47_02165) - 423835..424653 (-) 819 WP_002358531.1 MurR/RpiR family transcriptional regulator -
  N7M47_RS02170 (N7M47_02170) - 424809..425516 (+) 708 WP_002358533.1 N-acetylmannosamine-6-phosphate 2-epimerase -
  N7M47_RS02175 (N7M47_02175) - 425509..427011 (+) 1503 WP_002358534.1 PTS transporter subunit EIIC -
  N7M47_RS02180 (N7M47_02180) - 427022..427504 (+) 483 WP_002358535.1 PTS glucose transporter subunit IIA -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 17179.08 Da        Isoelectric Point: 4.6511

>NTDB_id=732748 N7M47_RS01935 WP_002365911.1 378201..378659(+) (ssb) [Enterococcus faecalis strain NY13412]
MINNVTLVGRLTKDPDLRYTQSGTAVGQFTLAINRNFTNANNEREADFINCVIWRKAAESLANYATKGTLIGLTGRIQTR
NYENQQGQRIYVTEVVTESFQLLESREVNEQRKEQATGKATFDKQSMDKPDPLDPFSPENSIVDISDNDLPF

Nucleotide


Download         Length: 459 bp        

>NTDB_id=732748 N7M47_RS01935 WP_002365911.1 378201..378659(+) (ssb) [Enterococcus faecalis strain NY13412]
TTGATTAATAACGTTACATTAGTTGGACGATTAACCAAAGACCCAGATTTAAGGTATACGCAAAGTGGAACAGCCGTAGG
TCAATTTACGTTGGCCATTAATCGCAACTTTACCAATGCTAACAATGAAAGGGAAGCAGATTTTATCAACTGTGTTATTT
GGCGGAAAGCTGCAGAGTCATTAGCAAATTATGCAACAAAAGGGACTCTGATCGGTTTAACTGGTCGCATTCAAACAAGA
AACTATGAGAATCAACAAGGCCAGCGTATTTATGTAACTGAGGTTGTCACAGAAAGCTTCCAACTATTAGAATCAAGAGA
AGTAAACGAGCAACGAAAAGAACAGGCTACAGGTAAAGCTACGTTTGATAAACAGTCAATGGATAAACCTGATCCTCTGG
ATCCATTTTCGCCAGAAAATAGCATAGTGGATATTTCTGATAATGACCTGCCGTTTTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VT46

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

56.471

100

0.632
  ssbA Bacillus subtilis subsp. subtilis str. 168

47.283

100

0.572
  ssbB Bacillus subtilis subsp. subtilis str. 168

56.604

69.737

0.395
  ssbB/cilA Streptococcus mitis NCTC 12261

50

76.316

0.382
  ssbB Streptococcus sobrinus strain NIDR 6715-7

53.774

69.737

0.375
  ssbB/cilA Streptococcus pneumoniae TIGR4

49.138

76.316

0.375
  ssbB/cilA Streptococcus pneumoniae Rx1

48.276

76.316

0.368
  ssbB/cilA Streptococcus pneumoniae D39

48.276

76.316

0.368
  ssbB/cilA Streptococcus pneumoniae R6

48.276

76.316

0.368
  ssbB/cilA Streptococcus mitis SK321

48.276

76.316

0.368