Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   N5O82_RS04810 Genome accession   NZ_CP104580
Coordinates   1027365..1028585 (+) Length   406 a.a.
NCBI ID   WP_125886211.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain GD04120     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1022365..1033585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O82_RS04785 (N5O82_04785) - 1022600..1023436 (-) 837 WP_185755105.1 hypothetical protein -
  N5O82_RS04790 (N5O82_04790) - 1023439..1024245 (-) 807 WP_260466110.1 ABC transporter permease -
  N5O82_RS04795 (N5O82_04795) - 1024623..1025090 (-) 468 WP_125886214.1 hypothetical protein -
  N5O82_RS04800 - 1025108..1025293 (-) 186 WP_125886213.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  N5O82_RS04805 (N5O82_04805) pilB 1025659..1027362 (+) 1704 WP_125886212.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N5O82_RS04810 (N5O82_04810) pilC 1027365..1028585 (+) 1221 WP_125886211.1 type II secretion system F family protein Machinery gene
  N5O82_RS04815 (N5O82_04815) pilD 1028589..1029455 (+) 867 WP_125886210.1 A24 family peptidase Machinery gene
  N5O82_RS04820 (N5O82_04820) coaE 1029587..1030195 (+) 609 WP_125886209.1 dephospho-CoA kinase -
  N5O82_RS04825 (N5O82_04825) yacG 1030192..1030389 (+) 198 WP_041771656.1 DNA gyrase inhibitor YacG -
  N5O82_RS04830 (N5O82_04830) - 1030402..1031082 (-) 681 WP_013981946.1 energy-coupling factor ABC transporter permease -
  N5O82_RS04835 (N5O82_04835) - 1031139..1032179 (-) 1041 WP_125886208.1 NADP(H)-dependent aldo-keto reductase -
  N5O82_RS04840 (N5O82_04840) rplM 1032600..1033028 (+) 429 WP_003287302.1 50S ribosomal protein L13 -
  N5O82_RS04845 (N5O82_04845) rpsI 1033044..1033436 (+) 393 WP_013981948.1 30S ribosomal protein S9 -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44587.60 Da        Isoelectric Point: 9.8360

>NTDB_id=730653 N5O82_RS04810 WP_125886211.1 1027365..1028585(+) (pilC) [Stutzerimonas stutzeri strain GD04120]
MAEKALKTSVFTWEGTDRKGGKVKGELAGQSPALIKAQLRKQGINPLKVRKKAGSLFGAGKKIKPMDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVAAGNSFAGSLRKKPQYFDDLYCNLVESGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFESVFQGFGAELPAFTMMVVQLSRWLQEWWYIVFFALFIAAYLFK
HAYKRSEKFRDSLDRLMLKLPIVGDILYKSVVARYARTLSTTFAAGVPLVDALDSVAGATGNVVFRNAVAKIRMDVSSGV
QLNFSMRTTGIFPTMAIQMTAIGEESGALDEMLDKLASFYEAEVDNMVDNLTTLMEPLIMAVLGVLVGGLIIAMYLPIFQ
LGSVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=730653 N5O82_RS04810 WP_125886211.1 1027365..1028585(+) (pilC) [Stutzerimonas stutzeri strain GD04120]
ATGGCGGAAAAAGCGTTGAAAACCAGTGTGTTCACCTGGGAAGGCACCGACAGAAAAGGTGGCAAGGTCAAGGGGGAGCT
GGCAGGTCAGAGCCCGGCACTGATCAAGGCGCAGCTCCGCAAGCAGGGTATCAATCCGCTGAAGGTTCGCAAGAAGGCCG
GTTCCTTGTTCGGGGCGGGCAAGAAAATCAAGCCGATGGACATCGCACTGTTCACTCGGCAAATGGCCACTATGATGAAG
GCAGGTGTGCCGCTGCTGCAATCGTTCGACATTATTGGCGAGGGCTTCGATAACCCAAATATGCGCAAGTTGGTCGATGA
GATCAAGCAAGAGGTGGCGGCAGGCAACAGCTTTGCTGGCTCGCTGCGTAAGAAGCCGCAATATTTCGATGACTTGTATT
GCAACTTAGTCGAGTCTGGAGAGCAGTCCGGTGCGCTCGAAACGTTGCTAGACCGTGTTGCTACCTACAAGGAGAAAACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCGATGACCTATCCGATTGCGGTTATCGTGGTGGCAATCATCGTATCGGCGAT
TCTCTTGATTAAGGTGGTCCCTCAGTTCGAAAGTGTATTTCAGGGCTTCGGCGCGGAACTTCCAGCCTTCACCATGATGG
TGGTGCAGTTGTCACGTTGGCTGCAGGAGTGGTGGTATATCGTATTCTTTGCATTATTCATTGCTGCCTATTTATTCAAG
CATGCATATAAGAGGTCGGAGAAATTTCGTGACTCTCTTGATCGCCTTATGCTCAAGCTGCCGATCGTTGGCGACATTCT
CTACAAGTCTGTAGTTGCGCGATATGCTCGCACATTGTCCACGACATTCGCGGCTGGTGTGCCCTTGGTTGATGCGTTGG
ACTCAGTGGCAGGTGCCACGGGCAACGTCGTGTTCCGCAATGCAGTAGCGAAGATTCGTATGGACGTATCGTCTGGTGTG
CAGCTCAATTTCTCCATGCGCACGACCGGCATTTTTCCCACCATGGCTATTCAAATGACCGCTATCGGCGAAGAGTCCGG
TGCGTTGGACGAAATGCTCGACAAACTGGCCAGTTTCTATGAGGCTGAGGTCGATAACATGGTGGATAACCTTACGACCC
TGATGGAACCGCTGATCATGGCCGTGCTCGGGGTTCTGGTCGGTGGCTTGATCATCGCGATGTATCTGCCGATCTTCCAG
TTGGGTAGTGTGGTCGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

82.963

99.754

0.828
  pilC Acinetobacter baylyi ADP1

63.547

100

0.635
  pilC Acinetobacter baumannii D1279779

62.469

99.754

0.623
  pilC Legionella pneumophila strain ERS1305867

55.051

97.537

0.537
  pilG Neisseria gonorrhoeae MS11

45.274

99.015

0.448
  pilG Neisseria meningitidis 44/76-A

45.274

99.015

0.448
  pilC Vibrio cholerae strain A1552

42.857

100

0.429
  pilC Vibrio campbellii strain DS40M4

40.75

98.522

0.401