Detailed information NTG0730654

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: N5O82_RS04815
• Genome accession: NZ_CP104580
• Coordinates: 1028589..1029455 (+)
• Length: 288 a.a.
• NCBI ID: WP_125886210.1
• Uniprot ID: -
• Organism: Stutzerimonas stutzeri strain GD04120
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1023589..1034455

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N5O82_RS04795 (N5O82_04795)	-	1024623..1025090 (-)	468	WP_125886214.1	hypothetical protein	-
N5O82_RS04800	-	1025108..1025293 (-)	186	WP_125886213.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
N5O82_RS04805 (N5O82_04805)	pilB	1025659..1027362 (+)	1704	WP_125886212.1	type IV-A pilus assembly ATPase PilB	Machinery gene
N5O82_RS04810 (N5O82_04810)	pilC	1027365..1028585 (+)	1221	WP_125886211.1	type II secretion system F family protein	Machinery gene
N5O82_RS04815 (N5O82_04815)	pilD	1028589..1029455 (+)	867	WP_125886210.1	A24 family peptidase	Machinery gene
N5O82_RS04820 (N5O82_04820)	coaE	1029587..1030195 (+)	609	WP_125886209.1	dephospho-CoA kinase	-
N5O82_RS04825 (N5O82_04825)	yacG	1030192..1030389 (+)	198	WP_041771656.1	DNA gyrase inhibitor YacG	-
N5O82_RS04830 (N5O82_04830)	-	1030402..1031082 (-)	681	WP_013981946.1	energy-coupling factor ABC transporter permease	-
N5O82_RS04835 (N5O82_04835)	-	1031139..1032179 (-)	1041	WP_125886208.1	NADP(H)-dependent aldo-keto reductase	-
N5O82_RS04840 (N5O82_04840)	rplM	1032600..1033028 (+)	429	WP_003287302.1	50S ribosomal protein L13	-
N5O82_RS04845 (N5O82_04845)	rpsI	1033044..1033436 (+)	393	WP_013981948.1	30S ribosomal protein S9	-
N5O82_RS04850 (N5O82_04850)	petA	1033691..1034284 (+)	594	WP_013981949.1	ubiquinol-cytochrome c reductase iron-sulfur subunit	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 32019.97 Da
• Isoelectric Point: 6.8283

>NTDB_id=730654 N5O82_RS04815 WP_125886210.1 1028589..1029455(+) (pilD) [Stutzerimonas stutzeri strain GD04120]
MIYELMASQVLAFVLSAAVLGLLVGSFLNVVIYRVPIMMQREWRMQAREYLDYPPEPIGERFNLLVPSSRCPHCGHRILA
WENIPLVSWLALRGKCSSCQAPISCRYPLVELACGVLSGYVAWHFGFSWQTGAMLLLTWGLLAMSMIDVDHQLLPDSLVL
PLLWLGLIANSFALFASLEDALWGAIAGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSVVGA
VLGSILLKVQRAESSTPIPFGPYLAIAGWIALLWGDRITESYLQFARF

Nucleotide

• Length: 867 bp

>NTDB_id=730654 N5O82_RS04815 WP_125886210.1 1028589..1029455(+) (pilD) [Stutzerimonas stutzeri strain GD04120]
ATGATCTACGAATTGATGGCCAGCCAAGTGCTGGCCTTTGTTTTGTCTGCTGCAGTGCTCGGTCTGTTGGTTGGCAGCTT
TCTCAATGTCGTCATCTACCGTGTGCCGATCATGATGCAGCGCGAATGGCGCATGCAGGCGCGGGAATATTTGGATTATC
CGCCCGAGCCAATTGGGGAGCGTTTCAATCTATTGGTGCCGAGTTCACGCTGCCCTCACTGCGGTCATCGTATCCTTGCC
TGGGAGAACATTCCCTTGGTCAGCTGGCTGGCGCTGCGTGGAAAGTGCTCGTCTTGTCAGGCGCCGATCAGCTGTCGATA
TCCGTTGGTTGAGCTGGCGTGTGGCGTGCTATCCGGATACGTGGCATGGCATTTCGGTTTTTCCTGGCAGACAGGGGCGA
TGCTTCTGCTGACCTGGGGGTTGCTGGCGATGAGCATGATCGATGTCGATCATCAGTTGCTGCCTGACTCGCTCGTGCTG
CCGCTCTTGTGGTTAGGGTTGATTGCCAACAGCTTTGCTCTGTTCGCCTCTCTCGAGGATGCGCTGTGGGGGGCGATAGC
CGGTTACCTGAGCCTATGGTCCGTTTATTGGCTGTTCAAGCTGGTCACAGGCAAAGAGGGTATGGGCTACGGCGATTTCA
AGCTGCTGGCGATGCTTGGTGCCTGGGGCGGCTGGCAGGTTCTGCCCCTCACTATCTTGCTGTCTTCGGTGGTCGGCGCC
GTTCTTGGCAGTATTTTGCTAAAGGTGCAGCGGGCTGAAAGCAGTACGCCCATTCCGTTCGGGCCGTATCTGGCGATAGC
GGGGTGGATTGCGTTGCTTTGGGGCGACCGAATTACCGAAAGCTACCTCCAGTTCGCTCGCTTCTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-243)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	56.934	95.139	0.542
pilD	Vibrio cholerae strain A1552	56.364	95.486	0.538
pilD	Acinetobacter baumannii D1279779	51.439	96.528	0.497
pilD	Acinetobacter nosocomialis M2	51.439	96.528	0.497
pilD	Neisseria gonorrhoeae MS11	53.232	91.319	0.486