Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N5O82_RS04805 Genome accession   NZ_CP104580
Coordinates   1025659..1027362 (+) Length   567 a.a.
NCBI ID   WP_125886212.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain GD04120     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1020659..1032362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O82_RS04780 (N5O82_04780) - 1021707..1022603 (-) 897 WP_125886216.1 ABC transporter ATP-binding protein -
  N5O82_RS04785 (N5O82_04785) - 1022600..1023436 (-) 837 WP_185755105.1 hypothetical protein -
  N5O82_RS04790 (N5O82_04790) - 1023439..1024245 (-) 807 WP_260466110.1 ABC transporter permease -
  N5O82_RS04795 (N5O82_04795) - 1024623..1025090 (-) 468 WP_125886214.1 hypothetical protein -
  N5O82_RS04800 - 1025108..1025293 (-) 186 WP_125886213.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  N5O82_RS04805 (N5O82_04805) pilB 1025659..1027362 (+) 1704 WP_125886212.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N5O82_RS04810 (N5O82_04810) pilC 1027365..1028585 (+) 1221 WP_125886211.1 type II secretion system F family protein Machinery gene
  N5O82_RS04815 (N5O82_04815) pilD 1028589..1029455 (+) 867 WP_125886210.1 A24 family peptidase Machinery gene
  N5O82_RS04820 (N5O82_04820) coaE 1029587..1030195 (+) 609 WP_125886209.1 dephospho-CoA kinase -
  N5O82_RS04825 (N5O82_04825) yacG 1030192..1030389 (+) 198 WP_041771656.1 DNA gyrase inhibitor YacG -
  N5O82_RS04830 (N5O82_04830) - 1030402..1031082 (-) 681 WP_013981946.1 energy-coupling factor ABC transporter permease -
  N5O82_RS04835 (N5O82_04835) - 1031139..1032179 (-) 1041 WP_125886208.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62332.34 Da        Isoelectric Point: 5.4611

>NTDB_id=730652 N5O82_RS04805 WP_125886212.1 1025659..1027362(+) (pilB) [Stutzerimonas stutzeri strain GD04120]
MNENAPLGGLARQLVLVGQLDEKIAQQAQQQALRNQVPLVTWLVQNKLVKSRVLVDIAGEQFGVPFFDLGALEKETQPKD
LVSEKLVRQHRALPLQRRGNKLYVAVSDPTNHQAISDIQFNTGLSVEAVLVEDDKLGEAIEKLFESTTSSLDGLNDSELD
GLDIELPGDAKEDDAAHNEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKTYRVRFRTDGMLHEVARPPVQLVSRISAR
LKVMASLDIAERRKPQDGRIKMKVSSSKSIDFRVNTLPTLWGEKIVMRILDSSSAKMGIDALGYEDDQKELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPSCKQEVNVPRDVLLREGFP
EARIGSFKLYGPVGCDSCKGGYKGRVGIYEVVKITPALQRLIMEEGNSIDIALQARNDGFNDLRTSGLVKAMNGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=730652 N5O82_RS04805 WP_125886212.1 1025659..1027362(+) (pilB) [Stutzerimonas stutzeri strain GD04120]
ATGAACGAAAACGCGCCCCTTGGCGGCCTGGCCCGGCAGCTGGTTCTCGTAGGCCAGCTCGACGAAAAAATCGCGCAGCA
AGCTCAGCAGCAAGCCTTGCGTAACCAGGTGCCTTTGGTGACTTGGTTGGTGCAGAACAAACTGGTCAAGAGTCGGGTGC
TTGTCGATATCGCCGGTGAGCAGTTTGGTGTTCCGTTCTTTGATTTGGGCGCGCTGGAGAAAGAGACCCAGCCGAAGGAT
CTGGTCAGCGAGAAGCTGGTGCGCCAGCATCGCGCGCTACCATTGCAGCGTCGCGGCAACAAGCTCTATGTGGCTGTATC
CGACCCTACCAATCATCAGGCGATCAGTGATATCCAGTTCAATACAGGACTTTCCGTCGAGGCCGTGCTGGTTGAGGACG
ACAAGCTCGGTGAAGCGATTGAAAAGCTCTTCGAATCGACAACCTCCTCCCTTGATGGGTTGAATGATTCCGAGCTGGAC
GGCTTGGATATCGAACTACCTGGCGACGCCAAGGAGGACGATGCGGCGCATAACGAAGCCGATGATGCGCCAGTGGTGCG
GTTCGTTAACAAGATGCTGCTCGATGCAATCAAGGGTGGTTCGTCCGATTTGCACTTCGAGCCCTATGAGAAGACCTATC
GGGTGCGTTTCCGCACAGACGGCATGCTGCACGAGGTTGCCCGCCCACCGGTCCAACTGGTCAGCCGTATCTCCGCGCGT
CTGAAAGTAATGGCCAGTCTGGATATCGCCGAGCGGCGTAAGCCCCAGGATGGGCGAATCAAGATGAAGGTGTCGAGCTC
CAAATCCATTGATTTTCGCGTCAATACGCTACCCACCTTGTGGGGCGAGAAGATCGTCATGCGAATCTTGGATTCCTCAA
GTGCCAAGATGGGGATCGATGCGCTGGGGTACGAGGATGACCAGAAGGAGCTCTATCTTAGCGCGCTCAAGCAGCCGCAA
GGGATGATTCTGGTTACCGGGCCGACAGGCTCGGGTAAGACCGTTTCACTGTACACCGGCCTAAATATCCTCAACACGGT
CGATGTGAATATTTCCACCGCTGAAGATCCGGTTGAAATCAATTTGGAGGGGATCAATCAGGTCAACGTAAATCCGAAGC
AGGGCATGGACTTCGCCCAGGCGCTTCGAGCGTTCCTGCGCCAGGATCCCGACATCATCATGGTGGGTGAGATTCGAGAT
CTCGAAACCGCCGAGATTGCCATCAAGGCGGCGCAGACCGGGCACATGGTGATGTCGACTCTGCATACCAATAGTGCTGC
GGAAACGCTTACGCGCTTGCGCAATATGGGGGTGCCGGCGTTCAACATCGCTACATCGGTCAATTTGATCATTGCTCAGC
GCCTGGCTCGCAAGCTGTGCCCCAGCTGTAAGCAGGAAGTTAACGTGCCGCGCGATGTTCTGCTCCGAGAAGGATTCCCT
GAGGCGCGAATCGGTTCCTTCAAACTCTATGGACCAGTCGGGTGCGATAGCTGCAAGGGAGGCTACAAGGGACGTGTCGG
TATTTATGAAGTGGTTAAAATCACGCCAGCCCTGCAGCGGCTTATCATGGAAGAAGGCAATTCCATCGATATCGCCTTGC
AGGCGCGTAACGATGGGTTCAATGACCTGCGCACTTCGGGTCTGGTTAAAGCCATGAACGGCGTCACCAGCCTGGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.298

100

0.596
  pilB Acinetobacter baylyi ADP1

58.761

99.647

0.586
  pilB Legionella pneumophila strain ERS1305867

54.895

100

0.554
  pilB Vibrio cholerae strain A1552

51.268

97.354

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

51.366

96.825

0.497
  pilB Vibrio campbellii strain DS40M4

51.184

96.825

0.496
  pilF Neisseria gonorrhoeae MS11

50.829

95.767

0.487
  pilF Thermus thermophilus HB27

38.664

100

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.449

94.18

0.381
  pilB/pilB1 Synechocystis sp. PCC 6803

39.768

91.358

0.363