Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GZI12_RS13045 Genome accession   NZ_AP019651
Coordinates   2751685..2753463 (-) Length   592 a.a.
NCBI ID   WP_162047332.1    Uniprot ID   -
Organism   Vibrio taketomensis strain C4III291     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2746685..2758463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZI12_RS13025 - 2747442..2748260 (-) 819 WP_162047328.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  GZI12_RS13030 - 2748394..2749854 (-) 1461 WP_162047329.1 SPOR domain-containing protein -
  GZI12_RS13035 aroB 2749884..2750972 (-) 1089 WP_162047330.1 3-dehydroquinate synthase -
  GZI12_RS13040 aroK 2751003..2751521 (-) 519 WP_162047331.1 shikimate kinase AroK -
  GZI12_RS13045 pilQ 2751685..2753463 (-) 1779 WP_162047332.1 type IV pilus secretin PilQ family protein Machinery gene
  GZI12_RS13050 pilP 2753482..2753997 (-) 516 WP_162047333.1 pilus assembly protein PilP Machinery gene
  GZI12_RS13055 pilO 2753987..2754577 (-) 591 WP_162047334.1 type 4a pilus biogenesis protein PilO Machinery gene
  GZI12_RS13060 pilN 2754570..2755148 (-) 579 WP_162047335.1 PilN domain-containing protein Machinery gene
  GZI12_RS13065 pilM 2755138..2755938 (-) 801 WP_332058805.1 type IV pilus assembly protein PilM -
  GZI12_RS21055 pilM 2755947..2756129 (-) 183 WP_332058806.1 hypothetical protein Machinery gene

Sequence

Protein


Download         Length: 592 a.a.        Molecular weight: 64779.46 Da        Isoelectric Point: 4.5583

>NTDB_id=73058 GZI12_RS13045 WP_162047332.1 2751685..2753463(-) (pilQ) [Vibrio taketomensis strain C4III291]
MRNGLNLTVQWIVKVGMLLSYMLFATQCLAQEAGNAFTSVDFRVDNEKNGKIVLELRDTQIMVDLKKTSQGLTIDLLNTT
IEEDQLKLFDVRDFATNVQTLELFREPNSVRMVAKIDGKFDYQYSLNRNFVEIVVSKPQIQAKEKEQTILDKPAKKMSIN
FQDIPVRNVLQLIADYNNFNLVVSDSVSGNLTLRLDGVPWTQVLDIILQVKGLDKRVDGNVVLVAPRAELEQREKQRLEN
ARLEDELGDLTSEIIKVNFANAAEIAEMIGGEQAISMLSDRGSITVDDRTNSLLIRDLKSNIDVVRSIIESLDIPVKQVQ
IEARIVTVNEGNLDDLGVRWSINSSDKVGATLEGNFAERGLYQSSNNSSNSGSNSGNTGSGSTSGSGSAAEGGDEIDDFL
NVNLGAGGNAASIAFQVAKLGSNTLLDLELSALQRESKAEVISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKK
AVLSLKVTPQITPDNRLVLDLSVTQDRPGEVVKVGTGEAVSINTQRIGTQVLVENGETVVLGGIYQYSISDTVEKVPLLG
DIPFLGNLFRHTYQNMGKSELLIFVTPKVVMQ

Nucleotide


Download         Length: 1779 bp        

>NTDB_id=73058 GZI12_RS13045 WP_162047332.1 2751685..2753463(-) (pilQ) [Vibrio taketomensis strain C4III291]
ATGAGAAATGGATTGAATTTAACCGTGCAGTGGATAGTCAAGGTTGGCATGTTGCTTAGCTACATGCTCTTTGCTACTCA
ATGTCTCGCTCAAGAGGCAGGTAATGCATTTACTAGTGTCGATTTTCGTGTAGATAACGAGAAAAATGGCAAGATTGTGC
TTGAATTGCGCGATACGCAAATCATGGTTGACCTGAAGAAAACCTCGCAAGGTCTGACGATTGATTTGTTGAATACCACC
ATCGAAGAAGATCAACTTAAGTTATTTGATGTGCGTGATTTTGCGACCAACGTGCAAACCTTGGAGCTATTTCGCGAGCC
AAACAGTGTGAGAATGGTGGCGAAAATTGATGGTAAGTTTGATTACCAATACTCGCTGAACCGTAACTTTGTTGAAATTG
TGGTGAGCAAGCCACAAATTCAAGCCAAAGAAAAAGAGCAAACCATTTTAGATAAGCCTGCGAAAAAGATGTCGATCAAC
TTCCAAGACATCCCAGTACGTAACGTATTGCAGCTTATTGCGGACTATAACAATTTCAACCTAGTAGTGTCTGACTCAGT
CTCAGGTAACCTGACTTTACGTCTCGATGGTGTGCCTTGGACTCAAGTACTCGACATCATTTTGCAAGTAAAAGGCCTGG
ATAAGCGTGTTGACGGTAACGTGGTATTGGTTGCTCCGCGTGCAGAACTTGAGCAACGTGAAAAACAACGTTTGGAAAAT
GCTCGCTTAGAAGATGAACTGGGTGATCTGACATCAGAAATTATTAAAGTGAATTTTGCTAATGCAGCTGAAATTGCAGA
GATGATTGGTGGTGAACAGGCGATTAGTATGTTGTCTGACCGTGGTTCTATTACGGTAGATGACCGCACCAACTCACTGT
TGATTCGCGATCTCAAGAGTAACATTGACGTCGTGCGCAGCATCATTGAATCGCTCGATATACCAGTGAAACAGGTACAA
ATCGAAGCCCGTATCGTGACGGTCAACGAAGGTAACCTTGATGATCTTGGTGTACGTTGGAGTATCAACTCGAGTGACAA
AGTGGGTGCTACATTAGAGGGGAATTTTGCTGAGCGCGGTTTGTATCAAAGCTCTAATAACAGCTCTAACTCTGGTTCTA
ACTCAGGAAATACGGGTTCAGGTAGCACATCAGGTTCAGGATCTGCGGCGGAAGGCGGTGACGAAATTGATGATTTCCTC
AATGTTAACTTAGGAGCGGGTGGTAACGCGGCGTCGATTGCGTTCCAAGTGGCTAAGTTGGGTTCCAATACACTGCTTGA
TCTTGAGCTTTCGGCATTGCAACGTGAGTCAAAGGCAGAAGTTATTTCTAGCCCAAGATTGATCACGACTAACAAAAAAC
CAGCATACATTGAGCAAGGTACGGAAATCCCTTATTTAGAATCGTCATCCAGCGGTGCAACCTCTGTGACGTTTAAGAAA
GCGGTATTGAGTCTTAAAGTGACACCACAAATCACGCCTGACAACCGTTTGGTGCTGGATTTGAGTGTGACTCAAGATAG
ACCAGGTGAAGTGGTGAAAGTCGGCACTGGCGAAGCGGTATCGATTAATACGCAACGCATTGGTACTCAAGTCTTGGTGG
AAAACGGTGAGACCGTCGTATTGGGTGGTATTTACCAATACAGTATCAGTGATACCGTAGAGAAAGTGCCGCTACTTGGA
GACATTCCGTTTCTTGGCAATTTGTTCCGTCACACCTACCAAAATATGGGTAAAAGTGAACTGTTAATCTTTGTCACGCC
GAAAGTAGTTATGCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(253-318)

Bacterial type II and III secretion system protein (Secretin)

(432-590)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

66.332

100

0.669
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

62.895

100

0.639
  pilQ Vibrio cholerae strain A1552

62.895

100

0.639
  pilQ Legionella pneumophila strain ERS1305867

40.989

95.608

0.392
  pilQ Legionella pneumophila strain Lp02

40.989

95.608

0.392
  pilQ Pseudomonas aeruginosa PAK

39.576

95.608

0.378
  comQ Acinetobacter baylyi ADP1

36.717

99.831

0.367

Multiple sequence alignment