Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   GZI12_RS13060 Genome accession   NZ_AP019651
Coordinates   2754570..2755148 (-) Length   192 a.a.
NCBI ID   WP_162047335.1    Uniprot ID   -
Organism   Vibrio taketomensis strain C4III291     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2749570..2760148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZI12_RS13035 aroB 2749884..2750972 (-) 1089 WP_162047330.1 3-dehydroquinate synthase -
  GZI12_RS13040 aroK 2751003..2751521 (-) 519 WP_162047331.1 shikimate kinase AroK -
  GZI12_RS13045 pilQ 2751685..2753463 (-) 1779 WP_162047332.1 type IV pilus secretin PilQ family protein Machinery gene
  GZI12_RS13050 pilP 2753482..2753997 (-) 516 WP_162047333.1 pilus assembly protein PilP Machinery gene
  GZI12_RS13055 pilO 2753987..2754577 (-) 591 WP_162047334.1 type 4a pilus biogenesis protein PilO Machinery gene
  GZI12_RS13060 pilN 2754570..2755148 (-) 579 WP_162047335.1 PilN domain-containing protein Machinery gene
  GZI12_RS13065 pilM 2755138..2755938 (-) 801 WP_332058805.1 type IV pilus assembly protein PilM -
  GZI12_RS21055 pilM 2755947..2756129 (-) 183 WP_332058806.1 hypothetical protein Machinery gene
  GZI12_RS13070 - 2756268..2758817 (+) 2550 WP_162047336.1 penicillin-binding protein 1A -
  GZI12_RS13075 oxyR 2758877..2759764 (-) 888 WP_162047337.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 22150.79 Da        Isoelectric Point: 10.4157

>NTDB_id=73061 GZI12_RS13060 WP_162047335.1 2754570..2755148(-) (pilN) [Vibrio taketomensis strain C4III291]
MLHRVNLFPWREQRRRVHQQRFFGMLVGSVVVAIVIQIALGAFLEHQKNMQHQRLGFLQAHIANLDFQLKKLKQLEVEHD
SLLTRLKVVEELQAQRNKTTEFMNVMPTLIPDGVYVDKIKMGDQQVEMVGISDTTSRLATMLDLLENSPRVRDVSMHSIV
HDKPRFGKKFQTFRVSFTFLIAEAGSQEVQGG

Nucleotide


Download         Length: 579 bp        

>NTDB_id=73061 GZI12_RS13060 WP_162047335.1 2754570..2755148(-) (pilN) [Vibrio taketomensis strain C4III291]
ATGCTTCATAGAGTCAATCTATTTCCATGGCGCGAGCAGCGCCGTCGTGTTCATCAACAGCGATTCTTTGGCATGTTGGT
GGGCAGTGTTGTCGTCGCGATTGTCATTCAAATTGCGTTGGGTGCTTTTCTTGAGCATCAAAAGAACATGCAGCATCAAC
GTTTAGGTTTTTTACAAGCGCATATTGCCAATCTTGATTTTCAATTGAAAAAACTTAAGCAGTTAGAGGTCGAGCATGAC
TCTTTGCTGACGCGTTTAAAAGTGGTGGAAGAGTTGCAAGCACAGCGCAACAAGACCACGGAATTTATGAATGTGATGCC
AACGTTAATCCCTGATGGGGTGTACGTCGACAAAATCAAAATGGGTGATCAGCAAGTTGAAATGGTGGGCATTAGTGATA
CCACTTCGAGGCTTGCGACAATGTTGGATTTATTAGAGAACTCACCGAGGGTTCGCGATGTTTCCATGCACTCAATTGTT
CATGATAAACCTCGATTCGGTAAGAAATTTCAAACTTTTAGGGTGTCCTTTACGTTCTTGATTGCCGAAGCTGGTTCGCA
GGAGGTGCAGGGTGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(104-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

56.684

97.396

0.552
  pilN Vibrio campbellii strain DS40M4

53.631

93.229

0.5

Multiple sequence alignment