Detailed information    

experimental Experimentally validated

Overview

Name   tfoS   Type   Regulator
Locus tag   GTF74_RS04205 Genome accession   NZ_CP047303
Coordinates   875140..878484 (+) Length   1114 a.a.
NCBI ID   WP_001911421.1    Uniprot ID   -
Organism   Vibrio cholerae O1 biovar El Tor strain E7946     
Function   chitin sensor   
Competence regulation

Function

TfoS is a novel chitin-sensing transcriptional regulator that represents the direct link between chitin and natural competence in V. cholerae.


Genomic Context

Location: 870140..883484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTF74_RS04180 (GTF74_04180) sucC 870261..871427 (+) 1167 WP_001048578.1 ADP-forming succinate--CoA ligase subunit beta -
  GTF74_RS04185 (GTF74_04185) sucD 871427..872299 (+) 873 WP_000025475.1 succinate--CoA ligase subunit alpha -
  GTF74_RS04190 (GTF74_04190) znuB 872406..873188 (-) 783 WP_000571528.1 zinc ABC transporter permease subunit ZnuB -
  GTF74_RS04195 (GTF74_04195) znuC 873181..873969 (-) 789 WP_000996146.1 zinc ABC transporter ATP-binding protein ZnuC -
  GTF74_RS04200 (GTF74_04200) znuA 874053..874937 (+) 885 WP_000952849.1 zinc ABC transporter substrate-binding protein ZnuA -
  GTF74_RS04205 (GTF74_04205) tfoS 875140..878484 (+) 3345 WP_001911421.1 AraC family transcriptional regulator Regulator
  GTF74_RS04210 (GTF74_04210) - 879256..879486 (+) 231 WP_000777870.1 ferrous iron transport protein A -
  GTF74_RS04215 (GTF74_04215) feoB 879483..881759 (+) 2277 WP_000877243.1 Fe(2+) transporter permease subunit FeoB -
  GTF74_RS04220 (GTF74_04220) - 881756..881986 (+) 231 WP_000602538.1 FeoC-like transcriptional regulator -
  GTF74_RS04225 (GTF74_04225) - 882248..882676 (-) 429 WP_000929906.1 HIT domain-containing protein -

Sequence

Protein


Download         Length: 1114 a.a.        Molecular weight: 126389.97 Da        Isoelectric Point: 6.7772

>NTDB_id=1151 GTF74_RS04205 WP_001911421.1 875140..878484(+) (tfoS) [Vibrio cholerae O1 biovar El Tor strain E7946]
MFRTLLYGCLLNILIPSLVLALDKSMSIFYPLPIQEQGKVIVAQNLYLGSDGGIWVHDVHGKVMYFDGQNVLPRRGSVLP
QLSSHIVYANSAFWAFEQHELFRTYPGGERELMLSLTPGTQIIQMGASNGMIWMVDGENFYSYEIRSGRVESLSLHELYQ
LNSASKVVINDAQFLVSKWALATNVGVYLSDEQGFAHVKNSGKHFIQKLYFSHKRRELLVGSKRGALVIDIENKRPPLDR
IGSSHVLSIAETEKEYWIGTEDGLFVYSFITGKIVQLESNNQDGYALLGKKIYAMVNDFQGGMWIGTDKGIFYYSLFGQQ
FTRYPAQALSNDGSKTPIGKIAALNSDGDYLAITAQGLYNFNFSDELRKHLIYPGKVNDFVIAQEHLWIATEKGLVRYNL
SRRSIDTPNLPLPLLQTAIQHLSLDSDGNLWGTSDYQIWSYSLSEKNYRDYGSEWMVKAFSPSRITLLAAVKDGIVIGTE
HGAYSLLEKQIRFDFSSHRYGEILQVAEDSSGHIWLVGSYGVFQWRKDQPEAVTVELIEENIQPLCIAESKQGMWLISTK
GISYYRNQELTKHFGAPYGLISNEFLSASCAVGEEQDNSSLIIGSQFGVVKTNTEELAVSNLPNIQVTFSQVSVNHTTKL
LGYRMSEPLKIAYGDSISFQFGALPSSRSQSLEYKLNDEQQWQRFDWALLNFDQLLPGKYRLKVRSANPTQRYRTEALFD
FEVLQPWYMTSIALVGYVLSVLGLLALALWWRTRMILAANRKLKAQVELKTSQLRHQSKIVLSNNHQLRKQLQLHHTILG
NVLDNIDASLRHLSSYAKVRAWSEFEAPFNKVKQELAQLHFMHRGDEDESKYHDLHLLIESALKSWQEEYSKALIKLTYS
GEPRFVEVRQFNLDVLFNAVLTDAIKRLYKHQELTIQLEVRNAQAVIVFSDFGAPIQNIDKTMVTLSGSSYPLEELVIRS
GGVLHVLALKERNVIELAWPLATLPELETIHKPEEGEQAAKVGIDEVEKEWLSKVEKLIELHYSDPNFTTSTAAQALYVS
ERSLQRRFKAATGKTFKESLNEVRLEKACQSLLSGAKIAQVAFDCGFNDPSYFSQRFKHHFGLSPSQFIEDQEN

Nucleotide


Download         Length: 3345 bp        

>NTDB_id=1151 GTF74_RS04205 WP_001911421.1 875140..878484(+) (tfoS) [Vibrio cholerae O1 biovar El Tor strain E7946]
GTGTTTCGCACATTGTTGTATGGCTGCTTGCTCAATATCCTGATCCCATCCTTGGTATTGGCATTAGATAAATCTATGTC
TATTTTCTATCCTTTGCCCATTCAAGAGCAGGGGAAAGTTATTGTTGCGCAAAATCTCTACCTCGGTAGTGATGGGGGGA
TTTGGGTACATGATGTTCATGGCAAAGTGATGTACTTTGATGGACAAAACGTCTTGCCTCGTCGTGGTTCTGTTCTGCCG
CAGTTGTCCAGTCATATTGTTTATGCGAACAGTGCATTTTGGGCTTTTGAGCAACACGAGTTGTTCAGAACCTATCCCGG
GGGAGAAAGAGAGCTGATGTTGAGCTTAACTCCGGGAACGCAGATTATTCAGATGGGGGCCTCAAATGGCATGATCTGGA
TGGTTGATGGGGAAAACTTCTATAGTTATGAGATCCGAAGTGGCCGCGTCGAATCCCTCTCGTTACATGAACTTTATCAG
CTCAATAGTGCCAGCAAGGTCGTGATTAACGATGCGCAGTTTTTAGTCTCGAAATGGGCTTTAGCGACTAATGTTGGGGT
GTATTTATCTGATGAACAAGGTTTTGCCCATGTAAAAAATTCTGGCAAGCACTTTATCCAAAAGCTCTATTTTTCCCATA
AGCGGCGAGAGCTGTTAGTTGGGTCTAAACGTGGCGCGTTAGTCATTGATATTGAAAATAAACGTCCACCACTGGATAGA
ATTGGTTCCTCCCATGTCCTCTCTATTGCGGAAACCGAGAAAGAGTACTGGATCGGTACAGAAGATGGGCTCTTTGTTTA
CTCTTTTATCACCGGGAAAATCGTGCAACTTGAGAGTAACAACCAAGATGGCTATGCCTTACTGGGCAAGAAAATCTACG
CCATGGTGAATGACTTTCAAGGTGGGATGTGGATAGGAACTGACAAGGGGATTTTTTATTACTCCCTATTTGGTCAGCAG
TTTACTCGGTATCCGGCGCAAGCATTATCTAACGACGGAAGTAAAACACCGATCGGTAAAATAGCAGCTCTTAACTCGGA
TGGAGACTATCTGGCGATAACCGCACAAGGCCTGTATAACTTTAATTTTTCAGATGAGCTACGTAAGCATCTTATCTATC
CAGGTAAAGTAAATGATTTTGTGATTGCCCAAGAGCATCTCTGGATCGCGACAGAGAAAGGTCTGGTTCGTTACAATCTC
AGTCGTCGTAGTATTGATACGCCCAATTTACCGCTCCCTTTGCTACAAACGGCTATTCAGCATCTGAGCTTAGATAGTGA
CGGTAACTTATGGGGGACAAGTGATTACCAAATATGGTCATACTCACTCTCTGAAAAAAATTATCGAGATTATGGTTCTG
AATGGATGGTGAAGGCTTTTTCTCCATCAAGGATCACTCTGCTTGCCGCAGTAAAAGACGGCATAGTGATTGGGACGGAA
CATGGCGCTTATTCCCTGCTTGAAAAACAGATCCGCTTTGATTTTTCGAGTCATCGCTATGGTGAGATTTTACAAGTTGC
TGAAGATTCTTCAGGCCACATTTGGCTTGTTGGCTCTTATGGGGTTTTTCAGTGGCGTAAAGATCAGCCCGAAGCTGTCA
CTGTCGAGTTAATTGAAGAGAATATACAGCCTTTGTGTATTGCTGAGTCTAAGCAGGGGATGTGGCTTATCTCCACCAAA
GGGATCAGTTATTACCGTAATCAAGAGCTCACTAAGCATTTTGGGGCTCCTTATGGACTGATCAGTAATGAGTTCTTGTC
TGCATCGTGTGCGGTGGGTGAGGAGCAAGATAACTCATCGTTGATCATAGGATCGCAATTTGGTGTAGTGAAAACCAATA
CAGAAGAATTAGCGGTGTCTAATTTGCCCAATATTCAGGTGACGTTTAGCCAAGTCAGCGTAAATCATACGACCAAGCTT
TTGGGGTATCGTATGTCCGAGCCACTGAAAATTGCTTATGGTGATTCGATCAGCTTTCAGTTTGGCGCTTTACCCTCTTC
ACGCAGCCAATCTTTAGAATACAAACTCAACGATGAACAGCAGTGGCAGCGCTTTGATTGGGCGCTGCTCAATTTTGATC
AACTTTTACCTGGTAAGTATCGTTTGAAAGTCCGTAGCGCTAATCCGACACAGCGATACCGAACTGAGGCCCTGTTTGAT
TTTGAAGTCTTACAGCCTTGGTATATGACCTCCATCGCGCTTGTCGGATATGTGCTGAGCGTGTTGGGGTTATTAGCACT
CGCTTTATGGTGGCGAACAAGAATGATATTGGCAGCTAACCGCAAACTCAAAGCGCAAGTGGAGCTCAAAACCAGTCAGT
TACGTCATCAAAGTAAAATTGTGCTGAGCAACAACCACCAACTTCGAAAGCAACTGCAGCTCCACCATACAATTCTTGGT
AACGTGTTAGACAATATTGATGCTTCATTGCGGCACTTATCTTCCTACGCCAAAGTACGAGCATGGAGTGAATTTGAAGC
ACCGTTCAACAAGGTGAAGCAAGAGTTGGCTCAACTGCATTTTATGCATCGCGGCGATGAGGATGAAAGCAAATATCATG
ATTTGCATCTTTTGATTGAGTCTGCATTGAAGAGTTGGCAGGAAGAGTACAGTAAAGCCTTGATTAAGCTAACTTACAGC
GGTGAGCCTCGCTTTGTGGAAGTCCGACAATTCAACCTTGATGTGTTATTTAATGCTGTATTAACGGATGCGATCAAACG
TTTGTATAAGCATCAAGAATTAACCATACAACTTGAAGTGAGAAATGCGCAGGCGGTTATCGTATTTTCTGATTTTGGTG
CCCCAATCCAAAACATAGACAAAACCATGGTGACGTTATCTGGTAGTAGTTATCCGCTTGAAGAGTTAGTCATTCGCAGT
GGTGGTGTATTGCATGTGCTCGCGTTGAAAGAGCGTAATGTGATTGAGTTAGCTTGGCCATTGGCAACCTTACCCGAGTT
AGAAACCATTCATAAACCAGAAGAAGGGGAACAGGCGGCTAAGGTTGGGATCGATGAGGTGGAGAAGGAGTGGCTAAGCA
AAGTCGAGAAACTGATTGAGCTGCATTATAGCGACCCCAATTTCACCACATCCACTGCAGCGCAGGCGCTTTATGTCTCT
GAACGCAGTTTACAGCGTCGCTTTAAAGCCGCGACAGGAAAAACCTTCAAAGAGTCGTTAAATGAGGTTCGCTTAGAAAA
AGCGTGTCAAAGCTTATTGAGTGGGGCGAAAATCGCACAAGTTGCCTTTGATTGCGGATTTAATGATCCCTCTTATTTCA
GTCAGCGTTTCAAACATCATTTTGGATTATCACCAAGCCAATTCATCGAAGATCAAGAAAACTAA

Domains

Predicted by InterproScan.

Helix-turn-helix domain (HTH_18)

(1034-1109)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Allison C Hullinger et al. (2025) Two transmembrane transcriptional regulators coordinate to activate chitin-induced natural transformation in Vibrio cholerae. PLoS Genetics 21(2):e1011606. [PMID: 39965000]
[2] Shouji Yamamoto et al. (2014) Regulation of natural competence by the orphan two-component system sensor kinase ChiS involves a non-canonical transmembrane regulator in Vibrio cholerae. Molecular Microbiology 91(2):326-47. [PMID: 24236404]
[3] Ankur B Dalia et al. (2014) Identification of a membrane-bound transcriptional regulator that links chitin and natural competence in Vibrio cholerae. MBio 5(1):e01028-13. [PMID: 24473132]