Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GZI12_RS13055 Genome accession   NZ_AP019651
Coordinates   2753987..2754577 (-) Length   196 a.a.
NCBI ID   WP_162047334.1    Uniprot ID   -
Organism   Vibrio taketomensis strain C4III291     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2748987..2759577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZI12_RS13035 aroB 2749884..2750972 (-) 1089 WP_162047330.1 3-dehydroquinate synthase -
  GZI12_RS13040 aroK 2751003..2751521 (-) 519 WP_162047331.1 shikimate kinase AroK -
  GZI12_RS13045 pilQ 2751685..2753463 (-) 1779 WP_162047332.1 type IV pilus secretin PilQ family protein Machinery gene
  GZI12_RS13050 pilP 2753482..2753997 (-) 516 WP_162047333.1 pilus assembly protein PilP Machinery gene
  GZI12_RS13055 pilO 2753987..2754577 (-) 591 WP_162047334.1 type 4a pilus biogenesis protein PilO Machinery gene
  GZI12_RS13060 pilN 2754570..2755148 (-) 579 WP_162047335.1 PilN domain-containing protein Machinery gene
  GZI12_RS13065 pilM 2755138..2755938 (-) 801 WP_332058805.1 type IV pilus assembly protein PilM -
  GZI12_RS21055 pilM 2755947..2756129 (-) 183 WP_332058806.1 hypothetical protein Machinery gene
  GZI12_RS13070 - 2756268..2758817 (+) 2550 WP_162047336.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22730.97 Da        Isoelectric Point: 4.8927

>NTDB_id=73060 GZI12_RS13055 WP_162047334.1 2753987..2754577(-) (pilO) [Vibrio taketomensis strain C4III291]
MAKYTELDVEDIVHWPVLAQVAVLAVLVAVLQAVGFWWYLDHKIEDLDQLKQQEIQLKQTVQIKANKVAALPQLQAQLDE
LSQRYDYLSRQLPVQKELATMLASVNEQGLKNSLTFTRIDWGEKQNQQFLYRLPLNIELTGDYNDIGEFSQAIAGLPRII
NFLDVEWHRVSLESSTLHFRVRAYTYQFKPEVKDEG

Nucleotide


Download         Length: 591 bp        

>NTDB_id=73060 GZI12_RS13055 WP_162047334.1 2753987..2754577(-) (pilO) [Vibrio taketomensis strain C4III291]
GTGGCTAAGTATACTGAGCTCGATGTCGAAGACATTGTTCATTGGCCCGTCTTGGCTCAAGTTGCGGTACTTGCAGTATT
AGTTGCAGTGCTGCAAGCGGTGGGTTTTTGGTGGTATCTCGATCATAAAATTGAAGATCTCGATCAGCTTAAGCAGCAAG
AAATTCAGCTCAAGCAGACGGTACAAATCAAAGCCAACAAAGTTGCCGCATTACCTCAACTGCAAGCTCAACTTGATGAG
TTATCGCAACGCTACGATTACTTATCACGCCAGCTTCCGGTGCAAAAAGAGTTAGCGACGATGTTGGCATCTGTAAACGA
ACAAGGTTTGAAAAACAGCCTGACTTTCACTCGTATCGACTGGGGTGAAAAGCAAAATCAGCAGTTCCTATATCGTTTAC
CGCTAAATATCGAATTGACTGGTGATTACAATGATATTGGTGAGTTCTCACAAGCTATCGCAGGGCTACCAAGGATCATC
AACTTTTTAGACGTGGAATGGCATCGCGTTAGTTTAGAGAGTAGCACGCTGCACTTTAGGGTTCGCGCTTATACCTACCA
GTTTAAACCGGAGGTGAAAGATGAAGGCTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

66.667

99.49

0.663
  pilO Vibrio campbellii strain DS40M4

61.579

96.939

0.597

Multiple sequence alignment